Bioconversion of de-oiled Jatropha Waste (DJW) to hydrogen and methane gas by anaerobic fermentation: Influence of substrate concentration, temperature and pH

Kumar, Gopalakrishnan; Lin, Ching-Nan

doi:10.1016/j.ijhydene.2012.10.053

Cited by 55 publications

(38 citation statements)

References 40 publications

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“…The main features of the inoculum were previously described elsewhere (Kumar and Lin, 2013). The cellulose (14.1%), hemicellulose (24.2%) and lignin (30.4) contents of the DJW were measured by the FIBERTEC TM analyzer as indicated in our previous study .…”

Section: Inoculum and Djwmentioning

confidence: 99%

“…The final working volume was made up with tap water. The composition of the nutrient solution used can be found elsewhere (Kumar and Lin, 2013). The vials were purged with argon for 5 min to provide a fully anaerobic environment.…”

Section: Experiments I: Selection Of Efficient Inoculum Pretreatment Mmentioning

confidence: 99%

“…The gas composition was analyzed by GC-TCD (China Chromatograph 8700T). All the measurement conditions are available in our previous work (Kumar and Lin, 2013). Kinetic analysis using the modified Gompertz equation was performed as previously reported (Kumar and Lin, 2013).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…All the measurement conditions are available in our previous work (Kumar and Lin, 2013). Kinetic analysis using the modified Gompertz equation was performed as previously reported (Kumar and Lin, 2013). Microbial community analysis (PCR-DGGE) was conducted by following the protocol described by Sivagurunathan et al, (2014).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…In our previous study, it was shown that the DJW could be successfully converted into hydrogen and methane by untreated, mixed microbial flora (Kumar and Lin, 2013). In this work, firstly, the selection of feasible seed pretreatment was attempted.…”

Section: Introductionmentioning

confidence: 99%

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Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach

et al. 2015

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Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment: process optimization by experimental design approach In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 o C, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 o C, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions. Kumar et al. / Biofuel Research Journal 5 (2015) 209-214 Please cite this article as: Kumar G., Bakonyi P., Sivagurunathan P., Nemestóthy N., Bélafi-Bakó K., Lin C.Y. Improved microbial conversion of de-oiled Jatropha waste into biohydrogen via inoculum pretreatment and process optimization by experimental design approach. Biofuel Research Journal 5 (2015) 209-214.

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Section: Inoculum and Djwmentioning

confidence: 99%

Section: Experiments I: Selection Of Efficient Inoculum Pretreatment Mmentioning

confidence: 99%