Bioconversion of industrial wastewater and wastewater sludge into Bacillus thuringiensis based biopesticides in pilot fermentor

Yezza, Abdessalem; Tyagi, Rajeshwar Dayal; Valero, José R. Lerma; Surampalli, Rao Y.

doi:10.1016/j.biortech.2005.08.023

Cited by 76 publications

(45 citation statements)

References 33 publications

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“…30-80 %, respectively using serial dilution and plate count methods [11][12][13][14]. Other studies have reported higher sporulation efficiencies or higher spore cell densities for other Bacillus species different from B. subtilis [3,6,15,16], but to our knowledge obtaining high values of both variables has not been reported yet.…”

Section: Introductionmentioning

confidence: 71%

“…Biomass concentration (g/L) increased during 10 h in the exponential phase reaching 3.5 g/L, consuming 96.9 % of reducing sugars and 92.0 % of dissolve oxygen. After 10 h of growth, cells entered a stationary phase and after 40 h of culture total biomass decline and dissolve oxygen reached 91.6 % suggesting the formation of spores [12,15]. Additionally in order to verify the optimization results at bioreactor level, spore cell density and sporulation efficiencies were determined at 50 h of culture obtaining 8.78 9 10 9 CFU/mL and 94.2 %, respectively.…”

Section: Logmentioning

confidence: 99%

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Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Posada-Uribe

Romero-Tabarez

Villegas-Escobar

2015

Bioprocess Biosyst Eng

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Bacillus subtilis spores have important biotechnological applications; however, achieving both, high spore cell densities and sporulation efficiencies in fermentation, is poorly reported. In this study, medium components and culture conditions were optimized with different statistical methods to increase spore production of the plant growth promoting rhizobacteria B. subtilis EA-CB0575. Key medium components were determined with Plackett-Burman (PB) design, and the optimum concentration levels of two components (glucose, MgSO4·7H2O) were optimized with a full factorial and central composite design, achieving 1.37 × 10(9) CFU/mL of spore cell density and 93.5 % of sporulation efficiency in shake flask. The optimized medium was used to determine the effect of culture conditions on spore production at bioreactor level, finding that maintaining pH control did not affect significantly spore production, while the interaction of agitation and aeration rates had a significant effect on spore cell density. The overall optimization generated a 17.2-fold increase in spore cell density (8.78 × 10(9) CFU/mL) and 1.9-fold increase in sporulation efficiency (94.2 %) compared to that of PB design. These results indicate the potential of B. subtilis EA-CB0575 to produce both, high spore cell densities and sporulation efficiencies, with very low nutrient requirements and short incubation period which can represent savings of process production.

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Section: Introductionmentioning

confidence: 71%

Section: Logmentioning

confidence: 99%

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Posada-Uribe

Romero-Tabarez

Villegas-Escobar

2015

Bioprocess Biosyst Eng

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“…Commercial Btk biopesticides products are normally based on international unit (IU) determined using cabbage looper larvae as target insect for bioassay [23]. In our earlier research, we found that spruce budworm unit (SBU) determined against spruce budworm is 20-25% higher than IU [24]. Therefore, the SBU measured in this research was converted to IU by using the above factor of conversion.…”

Section: Entomotoxicitymentioning

confidence: 97%

Optimization of spray drying process for Bacillus thuringiensis fermented wastewater and wastewater sludge

Adjallé

Tyagi

et al. 2010

Bioprocess Biosyst Eng

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Response surface methodology was used to optimize spray drying process for producing biopesticide powders of Bacillus thuringiensis by using fermented broth of starch industry wastewater and wastewater sludge. Analysis of variance was carried out using number of viable spores in the powder as dependent variable. The determination coefficients of models were 92 and 94% for fermented broth of starch industry wastewater and wastewater sludge, respectively. Under the optimal conditions of the operational parameters of spray drying, the numbers of viable spores were 2.2 × 10(8) and 1.3 × 10(8) CFU/mg in the dry powders for starch industry wastewater and wastewater sludge respectively, with a loss of viable spores of 18 and 13% when compared with their respective fermented broths. The entomotoxicity (measured by the bioassay method) of the powders obtained under optimal conditions showed a loss of 28 and 18% when compared with the fermented broth of starch industry wastewater and wastewater sludge, respectively. The optimized results of spray drying were used for field application calculations. The volume of fermented broth required to produce powder formulated product when compared with the volume required for liquid formulation product in order to treat 1 ha of balsam fir was less and offered several advantages.

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“…To develop a costeffective process for the production of Bt-based insecticide, it is imperative to cultivate the bacterial strain in a nutrient rich medium to obtain the highest yields of spore-crystal complexes. Conventionally, Bt-crystals are being produced employing submerged or liquid fermentation (SmF) techniques, but recently many workers use nutrient-rich waste water or sludge from various treatment plants as the medium for the production of Bt-toxin [74]. Solid-state fermentation: Solid-state fermentation (SSF) has been developed in eastern countries over many centuries, and has enjoyed broad application in these regions to date [75].…”

Section: Production Media and Media Formulationsmentioning

confidence: 99%

An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>

Jisha¹,

Smitha²,

Benjamin³

2013

AiM

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Strains of Bacillus thuringiensis (Bt) are known to produce crystalline proteins (δ-endotoxins) concomitantly with sporulation during their stationary phase of growth, which are demonstrated as lethal to lepidopeterous, coleopeterous and dipterous insects in addition to mites, nematodes, protozoa and flukes. Upon ingestion, the δ-nascent endotoxin is an inactive protoxin complex of (Cry alone or Cry and Cyt toxins together) high molecular mass, which is cleaved upon ingestion into the active component proteins at the high alkaline environments in the digestive tract of these agricultural pests. Conventionally, Bt-crystals are being produced employing submerged or liquid fermentation techniques in commercial media, but recently many workers have used solid-state fermentation strategy for the enhanced production of Bt-toxin at low cost. Apart from δ-endotoxin, some isolates of Bt produce another class of insecticidal small molecules called β-exotoxin (thuringiensin), which may be harmful to humans. Moreover, resistance to Bt developed in various target pest is yet another concern for Bt-industry. Following a brief introduction, this review addresses various toxins produced by various strains of Bt, Bt production media and media formulations with emphasis to solid-state fermentation, general structure of Cry toxin, its mode of action, target pests, bioassay, resistance to Bt toxins and resistance management. Briefly, this review would provide the readers an overview on the general aspects of Bt toxin, its general structure and mechanism of action.

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Bioconversion of industrial wastewater and wastewater sludge into Bacillus thuringiensis based biopesticides in pilot fermentor

Cited by 76 publications

References 33 publications

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Optimization of spray drying process for Bacillus thuringiensis fermented wastewater and wastewater sludge

An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>

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Bioconversion of industrial wastewater and wastewater sludge into Bacillus thuringiensis based biopesticides in pilot fermentor

Cited by 76 publications

References 33 publications

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Optimization of spray drying process for Bacillus thuringiensis fermented wastewater and wastewater sludge

An Overview on the Crystal Toxins from &lt;i&gt;Bacillus thuringiensis&lt;/i&gt;

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Resources

About

An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>