Bioconversion of tangerine residues by solid-state fermentation with Lentinus polychrous and drying the final products

Nitayapat, Nuttakan; Prakarnsombut, Namfon; Lee, Seung‐Ju; Boonsupthip, Waraporn

doi:10.1016/j.lwt.2015.03.016

Cited by 13 publications

(3 citation statements)

References 28 publications

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“…2D-SDS-PAGE. For two-dimensional gel electrophoresis, 13 cm pH 4−7 immobilized pH gradient (IPG) strips (GE Healthcare) were loaded with 80 μg of protein and then submitted to isoeletric focusing (IEF) in an Ettan IPGphor II system (GE Healthcare) for a total of 20 kV h at 20 °C according to manufacturer instructions. Reduction and alkylation of IPG gels were performed in 3 mL of equilibrium buffer (50 mM Tris pH 8.0, 6 M urea, 30% glycerol, and 2% SDS) containing 125 mM DTT for 30 min followed by incubation in the same buffer containing 300 mM acrylamide instead of DTT for 30 min.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Several species and strains have been tested as an alternative source of nutrients for humans and animals; 6−9 as bioactive compounds with antiparasitic, 10 antioxidant, 11−13 antinociceptive, and anti-inflammatory activity; 14 and also for different biotechnological applications such as production of hydrolytic and lignolytic enymes, 15−18 biodegradation of pollutants, 19 and bioconversion of biomass. 20,21 In the present work, we approached the characterization of the secretome of a local lignolytic Lentinus crinitus isolate. In order to favor the expression of the repertory of potential secreted enzymes, the fungus was grown in media with variable content of carbon, nitrogen, and water, and the resulting soluble extracts were analyzed through two-dimensional gel electrophoresis (2-DE), LC−MS, and conventional enzymatic assays.…”

Section: Introductionmentioning

confidence: 99%

“…Within the family of wood-rotting basidiomycetes, the Lentinus genus comprises a group of edible fungi with around 40 different species and worldwide distribution mainly in subtropical regions. Several species and strains have been tested as an alternative source of nutrients for humans and animals; − as bioactive compounds with antiparasitic, antioxidant, − antinociceptive, and anti-inflammatory activity; and also for different biotechnological applications such as production of hydrolytic and lignolytic enymes, − biodegradation of pollutants, and bioconversion of biomass. , …”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Biotechnological Potential of a Lentinus crinitus Isolate in the Light of Its Secretome

et al. 2016

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Analysis of fungal secretomes is a prospection tool for the discovery of new catalysts with biotechnological applications. Since enzyme secretion is strongly modulated by environmental factors, evaluation of growth conditions is of utmost importance to achieve optimal enzyme production. In this work, a nonsequenced wood-rotting fungus, Lentinus crinitus, was used for secretome analysis by enzymatic assays and a proteomics approach. Enzyme production was assessed after the fungus was cultured in seven different carbon sources and three nitrogen-containing compounds. The biomass yields and secreted protein arrays differed drastically among growing conditions. A mixture of secreted extracts derived from solid and liquid cultures was inspected by shotgun mass spectrometry and two-dimensional gel electrophoresis (2-DE) prior to analysis via LC-MS/MS. Proteins were identified using mass spectrometry (MS)-driven BLAST. The spectrum of secreted proteins comprised CAZymes, oxidase/reductases, proteases, and lipase/esterases. Although preseparation by 2-DE improved the number of identifications (162) compared with the shotgun approach (98 identifications), the two strategies revealed similar protein patterns. Culture media with reduced water content stimulated the expression of oxidases/reductases, while hydrolases were induced during submerged fermentation. The diversity of proteins observed within both the CAZyme and oxidoreductase groups revealed in this fungus a powerful arsenal of enzymes dedicated to the breakdown and consumption of lignocellulose.

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Section: Sample Preparationmentioning

confidence: 99%