Biodecolorization of Reactive Yellow‐2 by Serratia sp. RN34 Isolated from Textile Wastewater

Abstract

Remediation of colored textile wastewaters is a matter of interest. In this study, 49 bacteria were isolated from the textile wastewater and tested for their ability to decolorize reactive yellow-2 (RY2) dye. The most efficient isolate, RN34, was identified through amplification, sequencing, and phylogenetic analysis of its 16S rDNA and was designated as Serratia sp. RN34. This bacterium was also found capable of decolorizing other related reactive azo-dyes, including reactive black-5, reactive red-120, and reactive orange-16 but at varying rates. The optimum pH for decolorization of RY2 by the strain RN34 was 7.5 using yeast extract as cosubstrate under static incubation at 30 °C. The strain RN34 also showed potential to decolorize RY2 in the presence of considerable amounts of hexavalent chromium and sodium chloride. A phytotoxicity study demonstrated relatively reduced toxicity of RY2 decolorized products on Vigna radiata plant as compared to the uninoculated RY2 solution.

Enzymatic detoxification of azo dyes by a multifarious Bacillus sp. strain MR-1/2-bearing plant growth-promoting characteristics

“…A promising feature of this strain to perform its activity in the wastewaters is the onset of metabolic activity under abiotic stress conditions. Such metal tolerance in dye decolorizing bacterial strains was also reported in several previous studies (Hussain et al 2013;Najme et al 2015;Abbas et al 2016). Recently, it was found that thermophilic microflora were able to decolorize direct black G dye up to 97% in 8 h by simultaneously employing laccase, manganese peroxidase, lignin peroxidase, and azoreductase enzymes (Chen et al 2018).…”

“…The isolate MR-1/2 showed the maximum decolorization at a pH range of 6-8. It is common that the azo dye decolorization activity of bacterial strains is maximum near neutral to alkaline pH (Hussain et al 2013;Najme et al 2015;Abbas et al 2016). The percent deviation Table 5 Phytotoxicity evaluation of decolorized reactive red-120 with mungbean seeds NRR0 non-inoculated with no reactive red-120, NRR100 non-inoculated with 100 mg L −1 reactive red-120, NRR500 non-inoculated with 500 mg L −1 reactive red-120, WRR0 inoculated with MR-1/2 and no reactive red-120, WRR100 inoculated with MR-1/2 and 100 mg L −1 reactive red-120, WRR500 inoculated with MR-1/2 and 500 mg L −1 reactive red-120 **Different lower case letters represent significance between the treatments means (Fisher's LSD; P ≤ 0.05).…”

Effect of Reactive Black 5 azo dye on soil processes related to C and N cycling

“…Bioremediation of azo dyes in textile waste effluents in liquid media by bacteria has been widely studied ( Khalid, Arshad & Crowley, 2008 ; Khalid et al, 2013 ; Hussain et al, 2013 ; Anwar et al, 2014 ; Najme et al, 2015 ). Several species of bacteria have been identified which decolorize azo dyes ( Pandey, Singh & Iyengar, 2007 ; Hussain et al, 2013 ).…”

Characterization of a reactive yellow-2 decolorizing zinc tolerant bacterial strain Pseudomonassp. LT10 isolated from textile industry wastewater

“…As few fungal species have also been found in dye removal process (Hussain et al, 2017) but the bacterial strains are given priority because of their shorter life span and rapid degradation process (Verma and Madamwar, 2003;Elisangela et al, 2009;Imran et al, 2015). Previous scientist have isolated a number of bacteria belonged to different genera and studied their potential for decolorization of various azo dyes e.g., Enterobacter (Roy et al, 2018), Acinetobacter (Meerbergen et al, 2018), Arthrospira maxima (Afreen et al, 2016), Staphylococcus aureus (Ayed et al, 2017), Shewanella (Imran et al, 2014) and Serratia (Najme et al, 2015). Moreover the existence of metal ions including zinc, cadmium, chromium, copper, nickel etc.…”