Biodegradable Anti-Adhesive Membranes With Antibacterial Properties for Use in Surgery

Abstract

HighlightsAdhesion formation is a classic complication of any surgical intervention, which reduces the quality of life of patients and carries the risk of fatal complications during repeated surgery. Moreover, there is an issue of postoperative wound infection. Currently, there are no effective anti-adhesive agents with antibacterial activity in the arsenal of surgeons. Aim. To justify and develop biodegradable anti-adhesive membranes with antibacterial activity.Methods. The membranes were made by electrospinning using a composition of biodegradable polymers: polylactide-co-glycolide copolymer (50:50) Mm 20–30 KDa and ε-caprolactonone. The antibiotic Tigacil was added to the polymer solution to impart antibacterial properties to the membrane. The surface structure of the membranes was evaluated using a scanning electron microscope. Physical, mechanical and hemocompatibility properties were studied. The antibacterial activity of the membranes was evaluated after manufacturing, sterilization and storage for 24 months.Results. The inclusion of Tigacil in the membrane composition contributed to a decrease in fiber thickness by almost 4 times, while the packing of fibers became denser, which affected the increase in the strength of the modified membranes. Samples of membranes before and after the inclusion of Tigacil have no negative effect on erythrocytes The obtained results confirm the antibacterial activity of membranes against Staphylococcus aureus and Klebsiella pneumoniae, as well as the preservation of these properties after sterilization and during 2 years of storage.Conclusion. The inclusion of Tigacil in the polymer composition makes it possible to create biodegradable anti-adhesive membranes with antibacterial activity, which is maintained for two years after the manufacture. The inclusion of Tigacil in the polymer fiber composition does not adversely affect the initial properties of the membrane.