Biodegradable collagen patch with covalently immobilized VEGF for myocardial repair

Miyagi, Yasuo; Chiu, Loraine L. Y.; Cimini, Massimo; Weisel, Richard D.; Radišić, Milica; Li, Ren‐Ke

doi:10.1016/j.biomaterials.2010.10.007

Cited by 215 publications

(155 citation statements)

References 39 publications

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“…The desired number of cells (5000) were then suspended in 500 µl of culture medium per scaffold. The medium was then pipetted into the well containing the scaffold and the plate is placed on an orbital shaker in incubator at 37 ᵒC / 5 % CO 2 on 75 53 rpm setting for 1 hour. The culture medium was then aspirated and fresh medium was supplemented.…”

Section: The Inoculation Methods (Original Method)mentioning

confidence: 99%

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Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We hypothesized here that immobilizing a gradient of vascular endothelial growth factor would guide endothelial cells into the interior of the scaffold thereby enhancing angiogenesis. The protein was immobilized onto a collagen scaffold through carbodiimide chemistry by one of the three methods experimented: placing 5 µl of the solution at the center of the scaffold to create a ~2 ng/ml/mm gradient in a radial direction. D4T endothelial cells were observed to be guided by this VEGF-165 gradient deep into the center of the scaffold compared to both uniformly immobilized VEGF-165 and VEGFfree controls. We concluded that the VEGF-165 gradient scaffolds promoted the migration, and not proliferation, of cells deep into the scaffold. These gradient scaffolds provide the foundation for future in vivo tissue engineering studies.iii

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Section: The Inoculation Methods (Original Method)mentioning

confidence: 99%

“…Upon implantation of scaffolds with uniformly immobilized VEGF-165 into right ventricular wall defects in rat hearts, enhanced angiogenesis and patch stability was observed compared to VEGF-free controls [53]. We also uniformly immobilized VEGF-165 in combination with Angiopoetin-1 onto collagen scaffolds.…”

Section: Vascular Endothelial Growth Factormentioning

confidence: 99%

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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“…As a result, the affinity of some proteins and cellular response for a particular substrate is altered. Nowadays, different techniques aimed to modify scaffolds' surface have been developed, [15][16][17][18] among them surface functionalization with proteins or peptide sequences as well as the incorporation of an inorganic bioactive phase that triggers specific cell events have been successfully achieved.…”

Section: Importance Of Materials In 3d Printingmentioning

confidence: 99%

3D printed PLA-based scaffolds

et al. 2013

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“…As a result of VEGF immobilization, survival and proliferation of D4T endothelial cells were enhanced [121]. In a related study, Miyagi et al demonstrated increased angiogenesis and patch stability in defective rat hearts upon transplantation of scaffolds uniformly immobilized with VEGF, compared with VEGF-free controls [131]. In another study, VEGF and angiopoietin-1 (Ang-1) were co-immobilized onto collagen scaffolds [124].…”

Section: Covalent Surface Bondingmentioning

confidence: 99%

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Tallawi

Rosellini

Barbani

et al. 2015

J. R. Soc. Interface.

296

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The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers ( polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly-L-lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

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Biodegradable collagen patch with covalently immobilized VEGF for myocardial repair

Cited by 215 publications

References 39 publications

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

3D printed PLA-based scaffolds

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

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