Biodegradable Electrospun Nanofibrous Scaffolds for Bone Tissue Engineering

Anwar, Aneela; Petrino, Daniel Jerome; Alstine, Nicole Van; Yu, Xiaojun

doi:10.1007/978-1-0716-1811-0_36

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Multiple reports have demonstrated nanostructured scaffolds are more efficient than macrosized scaffolds in promoting bone regeneration [ 7 ]. Because nanoscaffolds have large surface areas and high porosity, they artificially mimic the extracellular matrix to a certain extent, providing an excellent natural environment for tissue regeneration processes, including cell adhesion, proliferation and differentiation [ 13 , 14 ]. Common nanofiber fabrication protocols comprise phase separation, self-assembly [ 15 ], melt blowing [ 16 ] and electrospinning [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Characteristic Evaluation of Recombinant MiSp/Poly(lactic-co-glycolic) Acid (PLGA) Nanofiber Scaffolds as Potential Scaffolds for Bone Tissue Engineering

Sun

Xing

Meng

2023

IJMS

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Biomaterial-based nanofibrous scaffolds are the most effective alternative to bone transplantation therapy. Here, two recombinant minor ampullate spidroins (spider silk proteins), R1SR2 and NR1SR2C, were blended with Poly(lactic-co-glycolic) Acid (PLGA), respectively, to generate nanofiber scaffolds by electrospinning. The N-terminal (N), C-terminal (C), repeating (R1 and R2) and spacer (S) modules were all derived from the minor ampullate spidroins (MiSp). The physical properties and structures of the blended scaffolds were measured by scanning electron microscopy (SEM), water contact angle measurement, Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), and Tensile mechanical testing. The results showed that blending of MiSp (R1SR2 and NR1SR2C) reduced the diameter of nanofibers, increased the porosity and glass transition temperatures of nanofibrous scaffolds, and effectively improved the hydrophilicity and ultimate strain of scaffolds. It is worth noting that the above changes were more significant in the presence of the N- and C-termini of MiSp. In cell culture assays, human bone mesenchymal stem cells (HBMSCs) grown on NR1SR2C/PLGA (20/80) scaffolds displayed markedly enhanced proliferative and adhesive abilities compared with counterparts grown on pure PLGA scaffolds. Jointly, these findings indicated recombinant MiSp/PLGA, particularly NR1SR2C/PLGA (20/80) blend nanofibrous scaffolds, is promising for bone tissue engineering.

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