Biodegradable Hydrogels for Time-Controlled Release of Tethered Peptides or Proteins

Brandl, Fabian; Hammer, Nadine; Blunk, Torsten; Teßmar, Jörg; Goepferich, Achim

doi:10.1021/bm901235g

Cited by 44 publications

(41 citation statements)

References 29 publications

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“…The adsorbed SST to AuNPs was determined using SDS-PAGE as previously reported (34)(35). In brief, 21µL of AuNPs-SST was mixed with 7µL modified SDS-PAGE sample buffer.…”

Section: Dissociation Of Sst From Aunps-sst By Solid Sodium Dodecyl Smentioning

confidence: 99%

Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors

Abdellatif

Zayed

Elbakry

et al. 2016

Drug Development and Industrial Pharmacy

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“…The adsorbed SST to AuNPs was determined using SDS-PAGE as previously reported (34)(35). In brief, 21µL of AuNPs-SST was mixed with 7µL modified SDS-PAGE sample buffer.…”

Section: Dissociation Of Sst From Aunps-sst By Solid Sodium Dodecyl Smentioning

confidence: 99%

Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors

Abdellatif

Zayed

Elbakry

et al. 2016

Drug Development and Industrial Pharmacy

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“…Additionally, HP hydrogels exhibited a 3D net structure and a good affinity and compatibility for biological tissues. An appropriately formulated HP hydrogel could encapsulate biological macromolecules and control the release of its contents in situ 2526. In the present study, an ASC was combined with an HP hydrogel to reconstruct a three-dimensional matrix structure.…”

mentioning

confidence: 97%

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Tian

et al. 2016

Sci Rep

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Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions. The bFGF-ASC complex was further encapsulated into a heparin modified poloxamer (HP) solution to prepare atemperature-sensitive hydrogel (bFGF-ASC-HP). bFGF release from the ASC-HP hydrogel was more slower than that from the bFGF-ASC complex alone. An in vitro cell survival study showed that the bFGF-ASC-HP hydrogel could more effectively promote the proliferation of PC12 cells than a bFGF solution, with an approximate 50% increase in the cell survival rate within 24 h (P < 0.05). Compared with the bFGF solution, bFGF-ASC-HP hydrogel displayed enhanced inhibition of glial scars and obviously improved the functional recovery of the SCI model rat through regeneration of nerve axons and the differentiation of the neural stem cells. In summary, an ASC-HP hydrogel might be a promising carrier to deliver bFGF to an injured spinal cord.

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“…61,62 The hydrolysis of phenyl carbamates in neutral and basic solutions proceeds by an E1cb elimination reaction involving the intermediate formation of an unstable isocyanate, which then disintegrates into a primary amine and carbon dioxide. This process releases the native protein from the polymer.…”

Section: E1cb Elimination Linkersmentioning

confidence: 99%

Releasable Conjugation of Polymers to Proteins

2015

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Many synthetic strategies are available for preparing well-defined conjugates of peptides/proteins and polymers. Most reports on this topic involve coupling methoxy poly(ethylene glycol) to therapeutic proteins, a process referred to as PEGylation, to increase their circulation lifetime and reduce their immunogenicity. Unfortunately, the major dissuading dogma of PEGylation is that, in many cases, polymer modification leads to significant (or total) loss of activity/function. One approach that is gaining momentum to address this challenge is to release the native protein from the polymer with time in the body (releasable PEGylation). This contribution will present the state-of-the-art of this rapidly evolving field, with emphasis on the chemistry behind the release of the peptide/protein and the means for altering the rate of release in biological fluids. Linkers discussed include those based on the following: substituted maleic anhydride and succinates, disulfides, 1,6-benzyl-elimination, host-guest interactions, bicin, β-elimination, biodegradable polymers, E1cb elimination, β-alanine, photoimmolation, coordination chemistry, zymogen activation, proteolysis, and thioesters.

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Biodegradable Hydrogels for Time-Controlled Release of Tethered Peptides or Proteins

Cited by 44 publications

References 29 publications

Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors

Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors

Thermo-sensitive hydrogels combined with decellularised matrix deliver bFGF for the functional recovery of rats after a spinal cord injury

Releasable Conjugation of Polymers to Proteins

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