Biodegradable microspherical implants containing teicoplanin for the treatment of methicillin-resistant Staphylococcus aureus osteomyelitis

Orhan, Zafer; Cevher, Erdal; Yildiz, Ayca; Ahıskalı, Rengin; Şensoy, Demet; Mülazımoğlu, Lütfiye

doi:10.1007/s00402-009-0886-9

Cited by 16 publications

(6 citation statements)

References 30 publications

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“…To induce 100% infection in osteomyelitis models, about 10 6 –10 8 CFU are required; in many studies ca. 2 × 10 6 CFU was used to stimulate osteomyelitis. ,, We used 4 × 10 6 and 8 × 10 6 CFU to develop severe osteomyelitis disease. On the basis of gross bone pathology analysis, extensive destructive lesions were observed in soft tissues with draining skin fistulas that had pus at the injured site.…”

Section: Discussionmentioning

confidence: 99%

Sustainable Release of Vancomycin from Silk Fibroin Nanoparticles for Treating Severe Bone Infection in Rat Tibia Osteomyelitis Model

Besheli

Mottaghitalab

Eslami

et al. 2017

ACS Appl. Mater. Interfaces

148

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The successful treatment of bone infections is a major challenge in the field of orthopedics. There are some common methods for treating bone infections, including systemic antibiotic administration, local nondegradable drug vehicles, and surgical debridement, and each of these approaches has advantages and disadvantages. In the present study, the antibiotic vancomycin (VANCO) was loaded in silk fibroin nanoparticles (SFNPs) and the complexes were then entrapped in silk scaffolds to form sustained drug delivery systems. The release kinetics of VANCO from SFNPs alone and when the SFNPs were entrapped in silk scaffolds were assessed at two different pH values, 4.5 and 7.4, that affected the release profiles of VANCO. Disk diffusion tests performed with pathogens causing osteomyelitis methicillin-resistant Staphylococcus aureus (MRSA) showed antibacterial activity of the released drug at two different pH values. Additionally, injection of 8 × 10 CFU MRSA in rat's tibia induced severe osteomyelitis disease. Radiographic and histopathological analyses were performed to evaluate the effectiveness of treatment after 6 weeks. The VANCO-loaded silk fibroin nanoparticles entrapped in scaffolds reduced bone infections at the defected site with better outcomes than the other treatment groups. In conclusion, the delivery system with good biocompatibility and sustained release properties would be appropriate for further study in the context of osteomyelitis disease.

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Section: Discussionmentioning

confidence: 99%

Sustainable Release of Vancomycin from Silk Fibroin Nanoparticles for Treating Severe Bone Infection in Rat Tibia Osteomyelitis Model

Besheli

Mottaghitalab

Eslami

et al. 2017

ACS Appl. Mater. Interfaces

148

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“…Most research focused on poly(lactide-co-glycolide) (PLGA). The in vitro and in vivo results of the related research showed that the antibiotic-PLGA microspheres were effective for the treatment of chronic osteomyelitis in animal experimental models [10][11][12]. In addition, Naraharisetti found that about 60% of gentamicin can be released from PLGA in approximately 5 to 6 days while the remaining drug is completely released in approximately 30 days [13].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Properties, and In Vitro Hydrolytic Degradation of Poly(d,l-lactide-co-glycolide-co-ε-caprolactone)

Liu

XiZhuang

Liang

2016

International Journal of Polymer Science

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Random copolymers of poly(d,l-lactide-co-glycolide-co--caprolactone) (PLGC) were synthesized by the ring-opening polymerization of d,l-lactide (DLLA), glycolide (GA), and -caprolactone (CL). The effects of CL on the copolymers were evaluated to prepare suitable copolymers with controlled properties. Our results showed that the CL content significantly influenced the thermal and mechanical properties of the copolymers and that the CL content in compositions could be altered to control properties of random copolymers. The in vitro hydrolytic degradation of the resulting implants showed that the degradation rate of PLGC was lower than that of PLGA, which could markedly reduce acidic degradation products. Finally, we demonstrated that higher CL contents in compositions slowed degradation rates.

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“…Implant-related osteomyelitis (IRO) is a complex bone infection with methicillin-resistant S. aureus (MRSA) as the main causative organism [ [23] , [24] , [25] ]. Despite new antibiotic regimens, knowledge of the disease, and new material development, peri-prosthetic joint and intramedullary infections are still devastating and a challenging complication in total joint arthroplasty and fracture management with significant impact on our socio-economic system and a patient's life [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Studies on the cytocompatibility, mechanical and antimicrobial properties of 3D printed poly(methyl methacrylate) beads

Mills

Jammalamadaka²,

Tappa³

et al. 2018

Bioactive Materials

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Osteomyelitis is typically a bacterial infection (usually from Staphylococcus) or, more rarely, a fungal infection of the bone. It can occur in any bone in the body, but it most often affects the long bones (leg and arm), vertebral (spine), and bones of the foot. Microbial success in osteomyelitis is due to their ability to form biofilms which inhibit the wound healing process and increases resistance to anti-infective agents. Also, biofilms do not allow easy penetration of antibiotics into their matrix making clinical treatment a challenge. The development of local antibiotic delivery systems that deliver high concentrations of antibiotics to the affected site is an emerging area of research with great potential. Standard treatment includes antibiotic therapy, either locally or systemically and refractory cases of osteomyelitis may lead to surgical intervention and a prolonged course of antibiotic treatment involving placement of antibiotic-doped beads or spacers within the wound site. There are disadvantages with this treatment modality including insufficient mixing of the antibiotic, lack of uniform bead size, resulting in lower antibiotic availability, and limitations on the antibiotics employed. Thus, a method is needed to address biofilm formations in the wound and on the surface of the surgical implants to prevent osteomyelitis. In this study, we show that all antibiotics studied were successfully doped into PMMA and antibiotic-doped 3D printed beads, disks, and filaments were easily printed. The growth inhibition capacity of the antibiotic-loaded PMMA 3D printed constructs was also demonstrated.

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Biodegradable microspherical implants containing teicoplanin for the treatment of methicillin-resistant Staphylococcus aureus osteomyelitis

Cited by 16 publications

References 30 publications

Sustainable Release of Vancomycin from Silk Fibroin Nanoparticles for Treating Severe Bone Infection in Rat Tibia Osteomyelitis Model

Sustainable Release of Vancomycin from Silk Fibroin Nanoparticles for Treating Severe Bone Infection in Rat Tibia Osteomyelitis Model

Synthesis, Properties, and In Vitro Hydrolytic Degradation of Poly(d,l-lactide-co-glycolide-co-ε-caprolactone)

Studies on the cytocompatibility, mechanical and antimicrobial properties of 3D printed poly(methyl methacrylate) beads

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