Biodegradable mucoadhesive particulates for nasal and pulmonary antigen and DNA delivery

Alpar, H. Oya; Somavarapu, Satyanarayana; Atuah, Kwame N; Bramwell, Vincent W.

doi:10.1016/j.addr.2004.09.004

Cited by 255 publications

(121 citation statements)

References 140 publications

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“…Strategies to improve the dosing, delivery, longevity, and transfection efficiency of DNA vaccines are receiving a great deal of attention. One method of facilitating delivery of plasmid DNA is through formulations that include incorporation of liposomes or polymers to extend plasmid longevity following injection and thereby enhance uptake over time and, subsequently, expression (5,32,53,111). Improved delivery methods include gene gun (49,145,153), BioJet (3,56,153), laser (168), ultrasound (146), and electroporation (63,151,160) approaches to enhance physical delivery.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform “Spotty Business”

Schoenly

Weiner

2008

J Virol

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Section: Nucleic Acidsmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform “Spotty Business”

Schoenly

Weiner

2008

J Virol

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“…Chitosan (CS), as a semi-crystalline aminopolysaccharide prepared by deacetylation of chitin, has been widely used in the biomedical field owing to its favorable properties such as non-toxicity, good biocompatibility, biodegradability, etc. (Bosquillon et al, 2001;Alpar et al, 2005;Yamamoto et al, 2005). Moreover, by means of a reduction of mucociliary clearance and the opening of tight intercellular junctions, chitosan is able to increase drug bioavailability after nasal administration (Vasir et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Preparation of honokiol-loaded chitosan microparticles via spray-drying method intended for pulmonary delivery

Guo

Zheng

et al. 2009

Drug Delivery

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R E S E A R C H A R T I C L EPreparation of honokiol-loaded chitosan microparticles via spray-drying method intended for pulmonary delivery IntroductionAt present, many drugs in clinic have to be administered by injection because of its poor oral bioavailability. However, injection route is not convenient for the patients due to poor compliance, pain, and etc. In the search for needle-free delivery, the administration of therapeutic drugs by inhalation is a promising alternative that has gained considerable interest over the past few years (Smith, 1997;Bosquillon et al., 2004). The lung was an attractive route for drug delivery because it was an efficient portal to the bloodstream, and it also could be the target organ. Besides, the lung could offer large absorptive surface area in the range of 35-140 m 2 , and thin (0.2 µm) and highly vascularized epithelium, which could lead to high bioavailability (Shoyele and Cawthorne, 2006). Moreover, inhalation is well accepted by the general population because of the convenience and good compliance (Byron, 1990).Preparation of dry powder formulations for inhalation is an attractive and appreciated proposal because solubility and stability issues of drugs can be resolved. Furthermore, a dry powder aerosol offers the capacity to provide a wide range of single dose per inhalation. The success of inhaled particles mostly depends on their size and density, and especially aerodynamic diameter (d ae ) (Grenha et al., 2005). Generally, the powder with aerodynamic diameter ranging from 1-5 µm could guarantee a maximum deposition in the deep lung. Particles size smaller than 1 µm failed to deposit in lung because it was easily breathed out again, and particle sizes larger than 5 µm also failed to deposit in the lung because they could not pass through the upper airways and cannot reach the deep lung (Broadhead et al.,1992;Bosquillon et al., 2001).Micronization is usually adopted to reduce the particle size of powder to less than 5 µm. Traditional method of micronization was using a ball or jet mill to produce powder. Whereas milling is a destructive Abstract It has been demonstrated that spray-drying is a powerful method to prepare dry powders for pulmonary delivery. This paper prepared dispersible dry powders based on chitosan and mannitol containing honokiol nanoparticles as model drug. The results showed that the prepared microparticles are almost spherical and have appropriate aerodynamic properties for pulmonary delivery (aerodynamic diameters was between 2.8-3.3 μm and tapped density ranging from 0.14-0. 18 g/cm 3 ). Moreover, surface morphology and aerodynamic properties of the powders were strongly affected by the content of mannitol. Fourier transform infra-red (FTIR) spectrum of powders indicated that the honokiol nanoparticles were successfully incorporated into microparticles. In vitro drug release profile was also observed. The content of mannitol in powders significantly influenced the release rate of honokiol from matrices. Keywords: Honokiol nanoparticles; spray-d...

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“…As a coating material, chitosan has been used for surface modification and as a mucoadhesive enhancer in the form of biodegradable microspheres carrying peptides and proteins for mucosal and oral delivery (20,21). The bioadhesive property of chitosan increased the residence time and prolonged the adsorption at mucosal site (22)(23)(24). The aims of this study were to formulate and evaluate chitosan-coated liposomal preparations including polyplex-loaded liposomes for DNA delivery via oral by investigating the characteristics and the stability in vitro using pRc/CMV-HBs.…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Chitosan-Coated Liposomes for Oral DNA Vaccine: The Improvement of Peyer’s Patch Targeting Using a Polyplex-Loaded Liposomes

et al. 2010

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Abstract. The aim of this study was to develop chitosan-coated and polyplex-loaded liposomes (PLLs) containing DNA vaccine for Peyer's patch targeting. Plain liposomes carrying plasmid pRc/CMV-HBs were prepared by the reverse-phase evaporation method. Chitosan coating was carried out by incubation of the liposomal suspensions with chitosan solution. Main lipid components of liposomes were phosphatidylcholine/cholesterol. Sodium deoxycholate and dicetyl phosphate were used as negative charge inducers. The zeta potentials of plain liposomes were strongly affected by the pH of the medium. Coating with chitosan variably increased the surface charges of the liposomes. To increase the zeta potential and stability of the liposome, chitosan was also used as a DNA condensing agent to form a polyplex. The PLLs were coated with chitosan solution. In vivo study of PLLs was carried out in comparison with chitosan-coated liposomes using plasmid encoding green fluorescence protein as a reporter. A single dose of plasmid equal to 100 μg was intragastrically inoculated into BALB/c mice. The expression of green fluorescence protein (GFP) was detected after 24 h using a confocal laser scanning microscope. The signal of GFP was obtained from positively charged chitosan-coated liposomes but found only at the upper part of duodenum. With chitosan-coated PLL540, the signal of GFP was found throughout the intestine. Chitosan-coated PLL demonstrated a higher potential to deliver the DNA to the distal intestine than the chitosan-coated liposomes due to the increase in permanent positive surface charges and the decreased enzymatic degradation.

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Biodegradable mucoadhesive particulates for nasal and pulmonary antigen and DNA delivery

Cited by 255 publications

References 140 publications

Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform “Spotty Business”

Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform “Spotty Business”

Preparation of honokiol-loaded chitosan microparticles via spray-drying method intended for pulmonary delivery

Development and Evaluation of Chitosan-Coated Liposomes for Oral DNA Vaccine: The Improvement of Peyer’s Patch Targeting Using a Polyplex-Loaded Liposomes

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