Biodegradable Polymer Nanoparticles of Anti-Tubercular Drug: Formulation and In Vitro Release Studies

Hakkimane, Sushruta S.; Guru, Bharath Raja

doi:10.1166/asl.2017.8493

Cited by 4 publications

(3 citation statements)

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“…In this study, RIF-and IH2-loaded NPs and NPs without drug were formed from PLGA polymer by single-emulsion solvent evaporation method using an optimized formulation. 34 PLGA 50:50 is biodegradable and hydrolytically cleavable into nontoxic oligomers or monomers of lactic acid and glycolic acid. [40][41][42] The unique characteristics of these drugencapsulated polymeric NPs are their biocompatibility, particle size and slow and sustained release of drug.…”

Section: Discussionmentioning

confidence: 99%

“…The RIF-encapsulated NPs were formulated using PLGA 50:50 by modified single-emulsion solvent evaporation method. 34 In brief, 90 mg of PLGA polymer and 45 mg of RIF were dissolved in 2 mL of chloroform, and this mixture was added to 12 mL of 2.5% PVA solution. The mixture was sonicated at 80% amplitude for 6-8 minutes with a pulse time of 10 seconds using a tip sonicator (Q-700 Ultrasonication Device; Q Sonica, Newtown, CT, USA) under an ice bath.…”

Section: Preparation Of Rif-and Ih2-loaded Plga Npsmentioning

confidence: 99%

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Antimycobacterial susceptibility evaluation of rifampicin and isoniazid benz-hydrazone in biodegradable polymeric nanoparticles against Mycobacterium tuberculosis H37Rv strain

Hakkimane¹,

Shenoy²,

Gaonkar³

et al. 2018

IJN

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IntroductionTuberculosis (TB) is the single largest infectious disease which requires a prolonged treatment regime with multiple drugs. The present treatment for TB includes frequent administration of a combination of four drugs for a duration of 6 months. This leads to patient’s noncompliance, in addition to developing drug-resistant strains which makes treatment more difficult. The formulation of drugs with biodegradable polymeric nanoparticles (NPs) promises to overcome this problem.Materials and methodsIn this study, we focus on two important drugs used for TB treatment – rifampicin (RIF) and isoniazid (INH) – and report a detailed study of RIF-loaded poly lactic-co-glycolic acid (PLGA) NPs and INH modified as INH benz-hydrazone (IH2) which gives the same therapeutic effect as INH but is more stable and enhances the drug loading in PLGA NPs by 15-fold compared to INH. The optimized formulation was characterized using particle size analyzer, scanning electron microscopy and transmission electron microscopy. The drug release from NPs and stability of drug were tested in different pH conditions.ResultsIt was found that RIF and IH2 loaded in NPs release in a slow and sustained manner over a period of 1 month and they are more stable in NPs formulation compared to the free form. RIF- and IH2-loaded NPs were tested for antimicrobial susceptibility against Mycobacterium tuberculosis H37Rv strain. RIF loaded in PLGA NPs consistently inhibited the growth at 70% of the minimum inhibitory concentration (MIC) of pure RIF (MIC level 1 µg/mL), and pure IH2 and IH2-loaded NPs showed inhibition at MIC equivalent to the MIC of INH (0.1 µg/mL).ConclusionThese results show that NP formulations will improve the efficacy of drug delivery for TB treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Rif-and Ih2-loaded Plga Npsmentioning

confidence: 99%

Antimycobacterial susceptibility evaluation of rifampicin and isoniazid benz-hydrazone in biodegradable polymeric nanoparticles against Mycobacterium tuberculosis H37Rv strain

Hakkimane¹,

Shenoy²,

Gaonkar³

et al. 2018

IJN

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“…A single emulsion solvent evaporation technique was used (Hakkimane et al, 2018) to formulate PTX loaded PLGA NPs, and CUR loaded PLGA NPs. Briefly, PLGA 50:50 (90 mgamount of polymer used), PTX (45 mg), or CUR (45 mg) 2:1 (Hakkimane and Guru, 2017) ratio of polymer. The drug (PTX and CUR were dissolved separately to formulate PTX loaded PLGA NPs and CUR loaded PLGA NPs) was first dissolved in 1.8 ml of chloroform (organic solvent).…”

Section: Preparation Of Paclitaxel/curcumin/coumarin-6 Loaded Plga Npsmentioning

confidence: 99%

Synergistic effect of Paclitaxel and Curcumin in nano-formulations on U87 and A549 cancer cell lines

Praveena¹,

S.²,

Raja³

2022

J App Pharm Sci

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Prolonged use of anticancer drug like Paclitaxel increases the level of transcription factor nuclear factor kappa B (NFƘB) which increases drug resistance in cancer cells. Multi-drug delivery using a combination of drugs that have different mechanisms of action has shown synergistic effects and NFƘB inhibition on cancer cells, though simultaneous delivery of more than one drug formulation for cancer treatment has not been studied deeply. The low aqueous solubility of most anti-cancer drugs limits their therapeutic effect. Polymeric Nano drug delivery is a promising path that can overcome the low solubility of chemotherapeutics. The present study focuses on the development of polylactide-co-glycolic acid nanoparticles (NPs) and incorporating them with Paclitaxel/Curcumin anti-cancer drugs by single emulsion solvent evaporation method and test their synergistic effect on cancer cells. The size and surface morphology of NPs were characterized by a particle size analyzer and a scanning electron microscope. The drug loading content analyzed using reverse-phase high-performance liquid chromatography was found to be 150 and 300 µg/mg for Paclitaxel and Curcumin, respectively. In vitro release study showed a slow and sustained release of the drug from the NPs. The formulated NPs as mono or in combination form were treated against U87 and A549 cells. They showed greater cytotoxicity, increased cellular uptake, and decreased NFƘB activity compared to free drugs in mono or combination form. The combination of drugs in nano-formulation showed greater cytotoxicity compared to mono drug NPs. From the results of the cytotoxicity assay, we can conclude that a combination of drugs in nanoformulation will be useful for cancer therapy.

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The Role of Nanomaterials in Diagnosis and Targeted Drug Delivery

Veg,

Hashmi,

Raza

et al. 2024

Chemistry & Biodiversity

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Nanomaterials owing to their small size imparts them with unique properties and they can also be designed and engineered according to the specific need. The use of nanoparticles (NPs) in medicine is particularly revolutionary as it has opened new therapeutic avenues to diagnose, treat and manage diseases in an efficient and timely manner. The review article presents the biomedical applications of nanomaterials including bioimaging, magnetic hypothermia and photoablation therapy, with a particular focus on disease diagnosis and targeted drug delivery. Nanobiosensors are highly specific and can be delivered into cells to investigate important biomarkers. They are also used for targeted drug delivery and deliver theranostic agents to specific sites of interest. The review also explores the role of nano‐based drug delivery systems for the management and treatment of nervous system disorders, tuberculosis and orthopaedics. The nano‐capsulated drugs can be transported by blood to the targeted site for a sustained release over a prolonged period. Some other applications like their role in invasive surgery, photodynamic therapy and quantum dot imaging have also been explored. Despite that, the safety concerns related to nanomedicine should be comprehended as well as the biodistribution of NPs in the body and the mechanistic insight.

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Biodegradable Polymer Nanoparticles of Anti-Tubercular Drug: Formulation and In Vitro Release Studies

Cited by 4 publications

References 0 publications

Antimycobacterial susceptibility evaluation of rifampicin and isoniazid benz-hydrazone in biodegradable polymeric nanoparticles against <em>Mycobacterium tuberculosis</em> H37Rv strain

Antimycobacterial susceptibility evaluation of rifampicin and isoniazid benz-hydrazone in biodegradable polymeric nanoparticles against <em>Mycobacterium tuberculosis</em> H37Rv strain

Synergistic effect of Paclitaxel and Curcumin in nano-formulations on U87 and A549 cancer cell lines

The Role of Nanomaterials in Diagnosis and Targeted Drug Delivery

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