Biodegradable thermogel as culture matrix of bone marrow mesenchymal stem cells for potential cartilage tissue engineering

Zhang, Yanbo; Ding, Jianxun; Xu, Weiguo; Wu, Jie; Chang, Fei; Zhuang, Xiuli; Chen, Xuesi; Wang, Jincheng

doi:10.1007/s10118-014-1551-5

Cited by 27 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrogels are physically or chemically cross-linked three-dimensional polymers swollen in water that allows the controlled release of bioactive agents (Park et al, 2009) and possesses the capacity of maintaining chondrocyte phenotype (Chung and Burdick, 2008; Spiller et al, 2011; Liu et al, 2018). Among various hydrogels, the thermo-sensitive hydrogel from PLGA–PEG–PLGA copolymer has been especially attractive, as their spontaneous gelation under physiological conditions presents some advantages: minimal invasive wounds, efficiency of cell encapsulation and controlled release of bioactive molecules (Zhang et al, 2014, 2015; Li et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment

et al. 2019

View full text Add to dashboard Cite

To provide a vehicle for sustained release of cartilage-protective agent for the potential application of osteoarthritis (OA) treatment, we developed a kartogenin (KGN)-incorporated thermogel for intra-articular injection. We fabricated a poly(lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PLGA–PEG–PLGA) thermogel as a KGN carrier for IA injection. OA chondrocytes were cultured in thermogel with or with no KGN to investigate the effect of KGN thermogel on cartilage matrix. The in vivo effect of KGN thermogel on OA was examined in a rabbit OA model. The KGN thermogel showed a sustained in vitro release of KGN for 3 weeks. OA chondrocytes proliferated well both in thermogel and KGN thermogel. In addition, OA chondrocytes produced higher amount of [type 2 collagen (COL-2) and glycosaminoglycan (GAG)], as well as lower level of matrix metalloproteinase 13 (MMP-13) in KGN thermogel that those in thermogel with no addition of KGN. The gene analysis supported that KGN thermogel enhanced expression of hyaline-cartilage specific genes Col 2 and AGC, and inhibited the expression of MMP-13. Compared with intra-articular injection of saline or thermogel containing no KGN, KGN thermogel can enhance cartilage regeneration and inhibit joint inflammation of arthritic knees in a rabbit ACLT-induced OA model at 3 weeks after the injection. Therefore, the KGN-incorporated PLGA–PEG–PLGA thermogel may provide a novel treatment modality for OA treatment with IA injection.

show abstract

Section: Discussionmentioning

confidence: 99%

Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment

et al. 2019

View full text Add to dashboard Cite

show abstract

“…PEG (M n = 1500 g mol − 1 ) was purchased from Sigma-Aldrich (Steinheim, Germany), and triblock PLGA-b-PEG-b-PLGA copolymer (M n, NMR = 4800 g mol −1 , LA/GA = 75:25, mol/mol) was synthesized through the approach reported in our previous works [30][31][32]. Briefly, the copolymer was synthesized through the ring-opening polymerization of LA and GA with PEG as a macroinitiator and stannous octoate as a catalyst.…”

Section: Methodsmentioning

confidence: 99%

Thermogel-mediated sustained drug delivery for in situ malignancy chemotherapy

Zhang

Ding

Sun

et al. 2015

Materials Science and Engineering: C

Self Cite

View full text Add to dashboard Cite

“…Among them, the thermogel based on poly(D,L-lactide- co -glycolide)- block -poly(ethylene glycol)- block -poly(D,L-lactide- co -glycolide) triblock copolymers (PLGA- b -PEG- b -PLGA) has shown outstanding potential for clinical applications due to their excellent tunable gelation behavior, biocompatibility and biodegradability [ 27 , 28 ]. In addition, our previous work confirmed that the PLGA- b -PEG- b -PLGA thermogel supports excellent adhesion and proliferation of BMMSCs [ 29 ]. In this work, the influence of intra-articular transplantation of exogenous BMMSCs entrapped in PLGA- b -PEG- b -PLGA thermogel accompanying with endogenous MSCs from bone marrow after microfracture on systemic immunosuppression and protection of surrounding cartilage under arthritic condition was clarified in rat model of collagen-induced arthritis (CIA), described as Fig.…”

Section: Introductionmentioning

confidence: 63%

Remission of Collagen-Induced Arthritis through Combination Therapy of Microfracture and Transplantation of Thermogel-Encapsulated Bone Marrow Mesenchymal Stem Cells

Ding

Wang

et al. 2015

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The persistent inflammation of rheumatoid arthritis (RA) always leads to partial synovial hyperplasia and the destruction of articular cartilage. Bone marrow mesenchymal stem cells (BMMSCs) have been proven to possess immunosuppressive effects, and widely explored in the treatment of autoimmune diseases. However, poor inhibitory effect on local inflammatory state and limited capacity of preventing destruction of articular cartilage by systemic BMMSCs transplantation were observed. Herein, toward the classical type II collagen-induced arthritis in rats, the combination treatment of microfracture and in situ transplantation of thermogel-encapsulated BMMSCs was verified to obviously down-regulate the ratio of CD4+ to CD8+ T lymphocytes in peripheral blood. In addition, it resulted in the decreased levels of inflammatory cytokines, such as interleukin-1β, tumor necrosis factor-α and anti-collagen type II antibody, in the serum. Simultaneously, the combination therapy also could inhibit the proliferation of antigen specific lymphocytes and local joint inflammatory condition, and prevent the articular cartilage damage. The results indicated that the treatment programs could effectively stimulate the endogenous and exogenous BMMSCs to exhibit the immunosuppression and cartilage protection capability. This study provided a new therapeutic strategy for autoimmune inflammatory diseases, such as RA.

show abstract

Biodegradable thermogel as culture matrix of bone marrow mesenchymal stem cells for potential cartilage tissue engineering

Cited by 27 publications

References 23 publications

Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment

Intra-articular Injection of Kartogenin-Incorporated Thermogel Enhancing Osteoarthritis Treatment

Thermogel-mediated sustained drug delivery for in situ malignancy chemotherapy

Remission of Collagen-Induced Arthritis through Combination Therapy of Microfracture and Transplantation of Thermogel-Encapsulated Bone Marrow Mesenchymal Stem Cells

Contact Info

Product

Resources

About