Biodegradable, thermoplastic polyurethane grafts for small diameter vascular replacements

Bergmeister, Helga; Seyidova, Nargiz; Schreiber, C; Strobl, Magdalena; Grasl, Christian; Walter, Ingrid; Meßner, Barbara; Baudis, Stefan; Fröhlich, S.; Marchetti‐Deschmann, Martina; Grießer, Markus; Franco, Matt di; Krššák, Martin; Liska, Robert; Schima, Heinrich

doi:10.1016/j.actbio.2014.09.003

Cited by 119 publications

(91 citation statements)

References 36 publications

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“…The material is based on classical poly(tetrahydrofuran) as soft-block and hexamethylene diisocyanate and bishydroxyethyl terephthalate as components for the hard-block in a molar ratio of 1:2:1. TPU grafts were fabricated via electrospinning and they were characterized morphologically and mechanically (fiber diameter; 1.39 ± 0.76 µ m, porosity; 74 ± 1%, pore size; 4.6 µ m, inner diameter; 1.6 mm, wall thickness; 78 ± 10 µ m) 4. EPTFE grafts (inner diameter; 1.5 mm, wall thickness; 100 μ m, intermodal-distance; 5–25 µ m, Zeuss, Orangeburg, USA) were used as controls.…”

Section: Methodsmentioning

confidence: 99%

“…TPU and ePTFE grafts (ID: 1.5 mm, length: 2 cm) were implanted into the infrarenal aorta of male Sprague–Dawley rats (300–400 g, n = 8 animals per time-point per group) using microsurgical techniques as previously described 4. No anti-coagulation nor anti-platelet drugs were administered.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, permanent presence of the foreign material in the host tissue will be avoided in these grafts 22. In our previous study a new biodegradable thermoplastic polyurethane polymer (TPU) with ultimate mechanical and functional behavior was synthesized and characterized 4. However, the inflammatory potential of the graft, which may evoke acute/chronic inflammatory responses at the site of implantation, still needs to be fully characterized.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

et al. 2016

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Following the implantation of biodegradable vascular grafts, macrophages and fibroblasts are the major two cell types recruited to the host-biomaterial interface. In-vitro biocompatibility assessment usually involves one cell type, predominantly macrophages. In this study, macrophage and fibroblast mono- and co-cultures, in paracrine and juxtacrine settings, were used to evaluate a new biodegradable thermoplastic polyurethane (TPU) vascular graft. Expanded-polytetrafluoroethylene (ePTFE) grafts served as controls. Pro/anti-inflammatory gene expression of macrophages and cytokines was assessed in vitro and compared to those of an in vivo rat model. Host cell infiltration and the type of proliferated cells was further studied in vivo. TPU grafts revealed superior support in cell attachment, infiltration and proliferation compared with ePTFE grafts. Expression of pro-inflammatory TNF-α/IL-1α cytokines was significantly higher in ePTFE, whereas the level of IL-10 was higher in TPU. Initial high expression of pro-inflammatory CCR7 macrophages was noted in TPU, however there was a clear transition from CCR7 to anti-inflammatory CD163 expression in vitro and in vivo only in TPU, confirming superior cell-biomaterial response. The co-culture models, especially the paracrine model, revealed higher fidelity to the immunomodulatory/biocompatibility behavior of degradable TPU grafts in vivo. This study established an exciting approach developing a co-culture model as a tool for biocompatibility evaluation of degradable biomaterials.Electronic supplementary materialThe online version of this article (doi:10.1007/s10439-016-1601-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

et al. 2016

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“…ventricle assist devices or heart valves [5]). Later on, bioresorbable PUs were introduced for various tissue constructs such as vascular grafts [6], cartilage [7], cancellous bone graft substitutes [8] or as grafts for small diameter vascular replacement [9]. Moreover, bioresorbable PUs can be tailored to possess a broad range of mechanical properties by selections and content of soft and hard segments [10,11].…”

Section: Introductionmentioning

confidence: 99%

Solvent free synthesis and structural evaluation of polyurethane films based on poly(ethylene glycol) and poly(caprolactone)

Kupka¹,

Vojtová²,

Fohlerová³

et al. 2016

Express Polym. Lett.

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Abstract. Biodegradable amphiphilic polyurethane films (bio-PUs) were synthesized by solvent free polyaddition reaction of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(caprolactone) (PCL) as macrodiols with hexamethylene diisocyanate. Samples were subsequently heat cured in order to obtain 3D crosslinked structure. Different PCL/PEG ratios allowed controlling the toughness of the resulting bio-PUs. Significant enhancement of Young's modulus, strength and elongation at break was observed at a PCL/PEG molar ratio above 3. The change in the bio-PU mechanical behavior was ascribed to the formation of crystalline PCL domains in the bio-PU network. The presence of PEG increased both the ability to absorb water and the rate of hydrolytic degradation, while PCL increased the cell viability. Prepared solvent free bio-PUs may advantageously be used in medicine as elastic resorbable material applicable against post-surgical adhesions.

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“…15 Therefore, porous TPU scaffolds after SBF nucleation can be readily employed for bone tissue regeneration owing to their ability to enhance cell adhesion and proliferation 16 and its biodegradability, which is readily absorbed and replaced by native cell populations. 17 For this purpose, this work evaluated a novel formulation of TPU/PVA composite filament as a 3D printable biomaterial for tissue-engineered bone scaffold fabrication. This is the first reported use of this unique TPU/PVA proprietary filament for use in bone tissue regeneration.…”

mentioning

confidence: 99%

Simulated Body Fluid Nucleation of Three-Dimensional Printed Elastomeric Scaffolds for Enhanced Osteogenesis

Castro

Tan

Shen

et al. 2016

Tissue Engineering Part A

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Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address current limitations regarding inadequate host tissue integration through the use of a novel elastomeric material for three-dimensional (3D) printing biomimetic and bioactive scaffolds. A novel thermoplastic polyurethanebased elastomeric composite filament (Gel-Lay) was used to manufacture porous scaffolds. In an effort to render the scaffolds more bioactive, the flexible scaffolds were subsequently incubated in simulated body fluid at various time points and evaluated for enhanced mechanical properties along with the effects on cell adhesion, proliferation, and 3-week osteogenesis. This work is the first reported use of a novel class of flexible elastomeric materials for the manufacture of 3D printed bioactive scaffold fabrication allowing efficient and effective nucleation of hydroxyapatite (HA) leading to increased nanoscale surface roughness while retaining the bulk geometry of the predesigned structure. Scaffolds with interconnected microfibrous filaments of *260 mm were created and nucleated in simulated body fluid that facilitated cell adhesion and spreading after only 24 h in culture. The porous structure further allowed efficient nucleation, exchange of nutrients, and metabolic waste removal during new tissue formation. Through the incorporation of osteoconductive HA, human fetal osteoblast adhesion and differentiation were greatly enhanced thus setting the tone for further exploration of this novel material for biomedical and tissue regenerative applications.

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Biodegradable, thermoplastic polyurethane grafts for small diameter vascular replacements

Cited by 119 publications

References 36 publications

Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

Solvent free synthesis and structural evaluation of polyurethane films based on poly(ethylene glycol) and poly(caprolactone)

Simulated Body Fluid Nucleation of Three-Dimensional Printed Elastomeric Scaffolds for Enhanced Osteogenesis

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