Biodegradable uniform microspheres based on solid-in-oil-in-water emulsion for drug delivery: A comparison of homogenization and fluidic device

Abstract

Based on solid-in-oil-in-water emulsification, we fabricated biodegradable poly(ϵ-caprolactone) microspheres containing gentamicin using conventional homogenization and a fluidic device. The feasibility of the poly(ϵ-caprolactone) microspheres as drug carriers was evaluated in terms of encapsulation efficiency, release behavior of gentamicin, and antimicrobial activity. The poly(ϵ-caprolactone) microspheres prepared using a fluidic device (fluidic device microspheres) had a uniform diameter and a smooth surface, whereas the poly(ϵ-caprolactone) microspheres prepared using conventional homogenization (conventional homogenization microspheres) exhibited polydisperse and a porous structure. At 0.3 wt% of gentamicin concentration, the encapsulation efficiencies of the conventional homogenization and fluidic device microspheres were 39.5% and 72.0%, respectively. In addition, a significant amount of gentamicin was only released initially from the conventional homogenization microspheres, whereas the fluidic device microspheres released gentamicin in a sustained manner for 28 days. These results confirmed the superior performances of the uniform fluidic device microspheres for drug delivery system. We further proposed a model for microsphere formation to explain the difference in performance of the conventional homogenization and fluidic device microspheres.

Chitosan/montmorillonite composite nanospheres for sustained antibiotic delivery at post-implantation bone infection treatment

“…Similarly, Ryu et al (2014) studied the effect of release kinetics on the antimicrobial activity of Gentamicin releasing PCL microspheres against S. aureus and E. coli. It was indicated that larger inhibition zones were achieved in the groups having burst drug release [35]. Similarly, Ambrogi et al (2017) observed that MMT incorporation in chitosan based films caused slightly lower antimicrobial activity due to slower antibiotic release caused by intercalation of drugs into clay layers [36].…”

“…In addition, the inhibition zone at the 6 h period was larger, which is attributed to the burst release in short-time period. Similarly, Ryu et al (2014) studied the effect of release kinetics on the antimicrobial activity of Gentamicin releasing PCL microspheres against S. aureus and E. coli. It was indicated that larger inhibition zones were achieved in the groups having burst drug release .…”

Comparison of thermal, structural and morphological properties of poly(L-lactide) and poly(L-lactide)/hydroxyapatite microspheres for laser sintering processes

“…The stirring of composite microspheres was carried out for 48 h at room temperature to allow the dichloromethane to evaporate. Finally, the composite microspheres were separated by centrifuging and were dried in vacuum at 40°C for 24 h [9,16].…”

Controlling the Dissolution Rate of Hydrophobic Drugs by Incorporating Carbon Nanotubes with Different Levels of Carboxylation

“…The solubility and bioavailability are typically improved by particle size reduction, which is described by the Noyes Whitney equation [2]. Typically, micro and nano drug particles are formed via mechanical size reduction such as dry/wet milling and homogenization [3], and also via precipitation techniques [4]. Anti-solvent precipitation has been used to synthesize micro and nano particles of hydrophobic drugs [5,6].…”