Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum

Thoa, Lê Thị Kim; Hung, Nguyen Bao; Khoo, Kuan Shiong; Quảng, Hoàng Tấn; Lan, Trần Thúy; Hoang, Vu Duc; Park, Seung-Moon; Ooi, Chien Wei; Show, Pau Loke; Huy, Nguyễn Đức

doi:10.1007/s12649-022-01692-2

Cited by 14 publications

(2 citation statements)

References 29 publications

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“…Pleurotus ostreatus, Trametes versicolor, and Ganoderma lucidum laccases are utilized for azo dye (Congo red) removal [9,18,19]. When Fusarium oxysporum laccase was present at a concentration of 100 mg/L, it was capable of eliminating 90% of malachite green [20].…”

Section: Introductionmentioning

confidence: 99%

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

Abd El-latif,

Zohri,

El-Aref

et al. 2024

Microb Cell Fact

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Background Azo dyes represent a common textile dye preferred for its high stability on fabrics in various harsh conditions. Although these dyes pose high-risk levels for all biological forms, fungal laccase is known as a green catalyst for its ability to oxidize numerous dyes. Methods Trichoderma isolates were identified and tested for laccase production. Laccase production was optimized using Plackett–Burman Design. Laccase molecular weight and the kinetic properties of the enzyme, including Km and Vmax, pH, temperature, and ionic strength, were detected. Azo dye removal efficiency by laccase enzyme was detected for Congo red, methylene blue, and methyl orange. Results Eight out of nine Trichoderma isolates were laccase producers. Laccase production efficiency was optimized by the superior strain T. harzianum PP389612, increasing production from 1.6 to 2.89 U/ml. In SDS-PAGE, purified laccases appear as a single protein band with a molecular weight of 41.00 kDa. Km and Vmax values were 146.12 μmol guaiacol and 3.82 μmol guaiacol/min. Its activity was stable in the pH range of 5–7, with an optimum temperature range of 40 to 50 °C, optimum ionic strength of 50 mM NaCl, and thermostability properties up to 90 °C. The decolorization efficiency of laccase was increased by increasing the time and reached its maximum after 72 h. The highest efficiency was achieved in Congo red decolorization, which reached 99% after 72 h, followed by methylene blue at 72%, while methyl orange decolorization efficiency was 68.5%. Conclusion Trichoderma laccase can be used as an effective natural bio-agent for dye removal because it is stable and removes colors very well.

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Section: Introductionmentioning

confidence: 99%

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

Abd El-latif,

Zohri,

El-Aref

et al. 2024

Microb Cell Fact

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“…35 In a study proposed by Show and his peers, extracellular laccase derived Fusarium oxysporum castoff in the direct degradation of Malachite Green. 36 Sodium dodecyl sulfate (SDS) is a surfactant that helps metals such as Cu and Mg etc. in preventing them from corrosion by attaching itself onto the active cathodic sites via producing an erosion protective layer, because of the extended hydrophobic extension of SDS.…”

Section: Introductionmentioning

confidence: 99%

Ag–Cu Embedded SDS Nanoparticles for Efficient Removal of Toxic Organic Dyes from Water Medium

Ali

Mehmood

Ashraf

et al. 2023

Ind. Eng. Chem. Res.

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Remediation of industrial water pollution is a trending subject to date for the researchers around the globe, due to its detrimental effects on human life as well as aquatic life. Azo dyes are the largest industrial water polluters in respect of volume while anthraquinone the second largest. Therefore, humongous considerations are being enumerated for the removal and decolorization of azo (AZ) dyestuff; however, for anthraquinone (AQ) dyes, these efforts are considerably minimal; although the later one poses a greater threat to environmental contamination, because of their reinforced structure. The current study is an effort toward this foremost issue. Chemical reduction synthesis of silver−copper (Ag−Cu) bimetallic nanoparticles (BNPs) was achieved using NaBH 4 as a reducing agent and sodium dodecyl sulfate (SDS) as a stabilizer. Characterization and morphological evaluation indicates two distinctive UV/vis absorption peaks for Ag and Cu. XRD studies for nanocomposite showed crystallite size of 8.96014 nm having an FCC structure. SEM with EDX confirmed the particle sizes of BNPs and SDS to be 17.14 and 114.56 nm, respectively. Potential catalytic activity and kinetic parameters of Ag−Cu BNPs@SDS were monitored against Methylene Blue (MB), Methyl Orange (MO), and eosin-y (EY). The percentage degradation recorded for the nanocatalyst against MO, MB, and EY was 95.21%, 98.57%, and 96.65%, respectively. This method can be adopted for the removal of multiple dyes from industrial effluent on a larger scale.

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Decolorization potential of malachite green by Ralstonia mannitolilytica isolated from Indonesian cassava-based fermented food tapai

Purnomo,

Victor,

Dikson

et al. 2023

Arch Microbiol

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Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum

Cited by 14 publications

References 29 publications

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

Kinetic studies on optimized extracellular laccase from Trichoderma harzianum PP389612 and its capabilities for azo dye removal

Ag–Cu Embedded SDS Nanoparticles for Efficient Removal of Toxic Organic Dyes from Water Medium

Decolorization potential of malachite green by Ralstonia mannitolilytica isolated from Indonesian cassava-based fermented food tapai

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