Biodegradation and post-oxidation of fuel-weathered field soil

Bajagain, Rishikesh; Gautam, Prakash; Jeong, Seung‐Woo

doi:10.1016/j.scitotenv.2020.139452

Cited by 19 publications

(2 citation statements)

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“…This can be explained because short-chain n-alkanes are cited to be toxic to many microorganisms [26], including heptane, octane, and nonane, which is an important factor to be considered in crude oil bioremediation by oleophilic microbes. C10-C18 n-alkanes seem to be the most biodegradable hydrocarbons, not only in this work, because other authors consider C18-C23 hydrocarbons difficult to be attacked by microorganisms [27,28]. In Figures 5 and 6, C21 n-alkane also appears as a recalcitrant hydrocarbon, difficult to be degraded.…”

Section: Crude Oil Degradationmentioning

confidence: 60%

Kinetics of Arab Light Crude Oil Degradation by Pseudomonas and Bacillus Strains

Costa

Santos

Vega

2022

Water

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The biodegradation of crude oil is a consequence of the presence of a specific enzymatic system in the microorganisms selected: the alkane hydroxylase (AlkH). The enzymatic biodegradation has been described since 1994, when the enzyme was first isolated from P. putida (formerly P. oleovorans), but the kinetics of microbial degradation has been weakly considered. We studied and described in this work the kinetics of Arab Light biodegradation, a light crude oil used for gasoline production (46.4% C7–C12 n-alkanes), using two oleophilic strains (Bacillus licheniformis and Pseudomonas putida). Alkanes were extracted from aqueous solutions in the bioreactors by dichloromethane, with a high ratio aqueous:organic volumes (1:0.2 mL) for the amplification of the GC n-alkane signals, and GC spectra were monitored in time over 40 days. Petroleum emulsions were visualized using optical microscopy as a result of biosurfactant segregation, which is necessary for the enzymatic biodegradation of oil by microorganisms. Kinetic analysis in biodegradation of Arab Light (total petroleum hydrocarbons, TPH) exhibits first-order kinetics with 0.098 d−1 and 0.082 d−1 as kinetic coefficients for 8.6 g/L initial crude oil concentration (30 °C), which results in degradation rates of 843 mg/Ld and 705 mg/Ld in B. licheniformis and P. putida, respectively. These results can be applied for oil spill bioremediation, using these microorganisms with the objective of removing contamination by petroleum alkanes.

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Section: Crude Oil Degradationmentioning

confidence: 60%

Kinetics of Arab Light Crude Oil Degradation by Pseudomonas and Bacillus Strains

Costa

Santos

Vega

2022

Water

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“…In situ techniques, in contrast, do not include excavation, transportation and isolated treatment of the contaminated sites. While ex situ methods, such as landfarming and biopiles, may incorporate bioremediation in the treatment process, as a rule, in situ techniques are gentler, less invasive, and thus have a less negative impact on the environment (Nikunen et al, 2017;USEPA, 2017;Bajagain et al, 2020a;Fernandez-Lopez et al, 2022). Various in situ methods offer important advantages, especially when traditional, off-site "dig and dump" approaches are ruled out by technical or economic demands.…”

Section: • Electric Heatingmentioning

confidence: 99%

Bioremediation of contaminated soil and groundwater by in situ biostimulation

Romantschuk,

Lahti-Leikas,

Kontro

et al. 2023

Front. Microbiol.

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Bioremediation by in situ biostimulation is an attractive alternative to excavation of contaminated soil. Many in situ remediation methods have been tested with some success; however, due to highly variable results in realistic field conditions, they have not been implemented as widely as they might deserve. To ensure success, methods should be validated under site-analogous conditions before full scale use, which requires expertise and local knowledge by the implementers. The focus here is on indigenous microbial degraders and evaluation of their performance. Identifying and removing biodegradation bottlenecks for degradation of organic pollutants is essential. Limiting factors commonly include: lack of oxygen or alternative electron acceptors, low temperature, and lack of essential nutrients. Additional factors: the bioavailability of the contaminating compound, pH, distribution of the contaminant, and soil structure and moisture, and in some cases, lack of degradation potential which may be amended with bioaugmentation. Methods to remove these bottlenecks are discussed. Implementers should also be prepared to combine methods or use them in sequence. Chemical/physical means may be used to enhance biostimulation. The review also suggests tools for assessing sustainability, life cycle assessment, and risk assessment. To help entrepreneurs, decision makers, and methods developers in the future, we suggest founding a database for otherwise seldom reported unsuccessful interventions, as well as the potential for artificial intelligence (AI) to assist in site evaluation and decision-making.

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