Biodegradation behavior of chitosan/calcium phosphate composites

Ding, Shinn-Jyh

doi:10.1016/j.jnoncrysol.2007.04.020

Cited by 44 publications

(22 citation statements)

References 37 publications

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“…There are reports suggesting that its degradation products do not cause any harmful effects in the organism (2,3,21,26). We encountered no toxic reactions in our present study, which correlated with data in the literature (1-3, 21, 26).…”

Section: Discussionsupporting

confidence: 91%

“…The effects of the membrane form versus the solution form of chitosan and the temporal effects of chitosan addition are presented. Assessment of the mitogenic activity of chitosan, especially high-deacetyl forms, found that high rates of mitosis were achieved at a 2.0% concentration (18)(19)(20)(21)(22). We used this concentration in our preliminary experiments to ensure standardisation with those prior studies.…”

Section: Resultsmentioning

confidence: 99%

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Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Uslu¹,

Biltekin²,

Denir³

et al. 2015

Balkan Med J

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Background: Chitosan, a linear polysaccharide, has been recently used in biomedical applications. In vitro studies have demonstrated its effect on cellular growth and its stimulatory action on cellular layer formation. Aims: The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. Study Design: In vitro study. Methods: Three experimental groups were formed: cells were cultured in a normal medium without chitosan (Control Group); cells were cultured either in a medium containing 2.0% chitosan in membranous form (Membrane Group) or chitosan solution at a concentration of 2.0% (Solution Group). Two different methods were used in the experiments: cells cultured on the medium containing chitosan in solution or membranous forms (method 1); and chitosan solution or membranous forms were added into the medium containing previously cultured cells (method 2). Results: Scanning electron microscopic investigations of the experimental groups revealed cells with welldefined cellular projections, intact cellular membranes and tight intercellular junctions. They were especially prominent in the membrane group of method 1 and in the membrane and solution groups of method 2. Mouse monoclonal anti-collagen 1 primary antibody was used to indicate collagen synthesis. Prominent collagen synthesis was detected in the membrane groups on the 10 th day of culture for both methods. Bromodeoxyuridine (BrdU) and MTT assays were performed in order to assess cellular proliferation and viability, respectively. BrdU labelling tests indicated a higher proliferation index in the membrane group of method 1 on the 5 th and 10 th days. For the second method, the membranous form on the 10 th day and solution form on the 5 th day were the most effective groups in terms of cellular proliferation. MTT results reflected a high cellular viability in method 1 on the 5 th day of treatment with the membranous form, whereas cellular viability was highest in the solution form of method 2 on the 5 th day. Conclusion: The membranous form of chitosan induced a significant proliferative effect and increased the ratio of cell-to-cell junctions of Swiss 3T3 mouse embryonic fibroblasts. Conveniently, the solution form also resulted in enhanced cell proliferation and viability compared to the control group. As the solution form is easy to prepare and apply to cells compared to the membrane form, the application of Chitosan directly to media appears to be a convenient alternative for tissue engineering approaches.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Uslu¹,

Biltekin²,

Denir³

et al. 2015

Balkan Med J

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“…However, it is inherently non-degradable in mammals (Lee and Mooney, 2012). Biodegradation of materials can be characterized by changes in their mechanical and physicochemical properties (disintegration and dissolution) (Ding, 2007). Ionically cross-linked alginate gels can be dissolved by release of the divalent ions due to exchange reactions with monovalent cations such as Na + (Lee and Mooney, 2012).…”

Section: Discussionmentioning

confidence: 99%

Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering

Qiao

Dong

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To deliver cells deep into injectable calcium phosphate cement (CPC) through alginate-chitosan (AC) microcapsules and investigate the biological behavior of the cells released from microcapsules into the CPC. Methods: Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet generator. The two types of cell-encapsulating microcapsules were then mixed with a CPC paste. MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy. Results: Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste. The released cells attached to the setting CPC scaffolds, survived, differentiated, and formed mineralized nodules. Cells grew in the pores concomitantly created by the AC microcapsules in situ within the CPC. At Day 21, cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group (P<0.05). Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate microcapsules. Conclusions: AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.

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“…As proven, calcium phosphate materials are osteoconductive to mimic the inorganic portion of natural bone, while CTS / HAp composite materials show promise in mimicking the organic portion as well as the inorganic portion of natural bone (Figure 1). Several studies have been conducted with CTS / HAp composite materials for bone tissue engineering [140][141][142][143][144][145]. Calcium phosphate compounds are of great interest in the field of bone tissue engineering.…”

Section: Composite Materials For Bone Tissue Engineeringmentioning

confidence: 99%

Temporomandibular Disorders and BIO-IPNs: in vitro approach to find molecular solution to biological problem

Perchyonok¹,

Grobler²,

Basson³

et al. 2016

Nanomaterials and Regenerative Medicine

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Biodegradation behavior of chitosan/calcium phosphate composites

Cited by 44 publications

References 37 publications

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering

Temporomandibular Disorders and BIO-IPNs: in vitro approach to find molecular solution to biological problem

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