Biodegradation kinetics of naphthalene in nonaqueous phase liquid-water mixed batch systems: Comparison of model predictions and experimental results

Ghoshal, Subhasis; Luthy, Richard G.

doi:10.1002/(sici)1097-0290(19980205)57:3<356::aid-bit12>3.0.co;2-e

Cited by 23 publications

(15 citation statements)

References 37 publications

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“…Many investigators such as Hussein (1997Hussein ( , 1999, Chrysikopoulos and Kim (2000), Chrysikopoulos et al (2002), Ghoshal andLuthy (1998), andIllangasekare et al (2000) have demonstrated the complex flow and transport behavior of non-aqueous phase liquids in porous media. Kueper and Frind (1989) developed a two-dimensional vertical section finite difference model to study the simultaneous movement of a dense, non-aqueous phase liquid and water under the water table in heterogeneous porous media.…”

Section: Introductionsupporting

confidence: 77%

Remediation of saturated soil contaminated with petroleum products using air sparging with thermal enhancement

Mohamed

El-menshawy

Saif

2007

Journal of Environmental Management

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Section: Introductionsupporting

confidence: 77%

Remediation of saturated soil contaminated with petroleum products using air sparging with thermal enhancement

Mohamed

El-menshawy

Saif

2007

Journal of Environmental Management

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“…Similar to the isotope-trapping experiment with strain P15, it is likely that the rate of formation of 4,5-dihydroxypyrene at high concentrations of PYRdHD exceeds the subsequent rate of metabolism, resulting in the accumulation of 4,5-dihydroxypyrene and its consequent autoxidation to PYRQ. A similar phenomenon has been noted before in the bacterial degradation of naphthalene, for which the accumulation of naphthoquinone has been suggested to increase as the rate of naphthalene availability increased (2,19). Strain PYR-1 is able to consume PYRQ as a growth substrate, which suggests that it would only accumulate transiently if formed by this organism or by other bacteria that might also be transforming pyrene in a complex system.…”

Section: Discussionmentioning

confidence: 99%

Products from the Incomplete Metabolism of Pyrene by Polycyclic Aromatic Hydrocarbon-Degrading Bacteria

Kazunga

Aitken

2000

Appl Environ Microbiol

161

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Pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (PAH). It is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other PAHdegrading bacteria. We examined the formation of such products by four bacterial strains and identified and further characterized the most apparently significant of these metabolites. Pseudomonas stutzeri strain P16 and Bacillus cereus strain P21 transformed pyrene primarily to cis-4,5-dihydro-4,5-dihydroxypyrene (PYRdHD), the first intermediate in the known pathway for aerobic bacterial mineralization of pyrene. Sphingomonas yanoikuyae strain R1 transformed pyrene to PYRdHD and pyrene-4,5-dione (PYRQ). Both strain R1 and Pseudomonas saccharophila strain P15 transform PYRdHD to PYRQ nearly stoichiometrically, suggesting that PYRQ is formed by oxidation of PYRdHD to 4,5-dihydroxypyrene and subsequent autoxidation of this metabolite. A pyrene-mineralizing organism, Mycobacterium strain PYR-1, also transforms PYRdHD to PYRQ at high initial concentrations of PYRdHD. However, strain PYR-1 is able to use both PYRdHD and PYRQ as growth substrates. PYRdHD strongly inhibited phenanthrene degradation by strains P15 and R1 but had only a minor effect on strains P16 and P21. At their aqueous saturation concentrations, both PYRdHD and PYRQ severely inhibited benzo[a]pyrene mineralization by strains P15 and R1. Collectively, these findings suggest that products derived from pyrene transformation have the potential to accumulate in PAH-contaminated systems and that such products can significantly influence the removal of other PAH. However, these products may be susceptible to subsequent degradation by organisms able to metabolize pyrene more extensively if such organisms are present in the system. Polycyclic aromatic hydrocarbons (PAH) are known to be degradable by a variety of soil bacteria (40). Consequently, the bioremediation of PAH contamination with naturally occurring microorganisms has been attempted at a number of sites (43,45). Most of the interest in the biodegradation of PAH in the field has been in the removal of the parent compounds, while most research on pure cultures of PAH-degrading bacteria has focused on their ability to grow on or mineralize specific PAH substrates. Relatively little attention has been paid to the potential formation of products from the partial transformation of PAH.Most of the information that does exist on PAH metabolites has been obtained in the context of identifying transient metabolites formed by isolates during growth on the parent compound (13,33,35,40) or metabolites formed by mutants of wild-type degraders (4, 40). Some bacteria, however, are capable of transforming one or more PAH despite an inability to grow on or mineralize the PAH in question (1,20,31,47). It is important to evaluate the products of such incomplete PAH metabolism because of their potential effects on PAH-degrading microorganisms or on potentially exposed human populations (44). Identification of common pr...

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“…In some cases, additional parameters were used to describe the system. For instance, Ghoshal and Luthy (1998) and Mukherji and Weber (1998) considered the mass transfer of naphthalene from the organic phase to the aqueous phase. The aforementioned model was based on the premise that microorganisms can only metabolise TPH when it is in the dissolved state; thus, these models can be used to determine if biodegradation is controlled by the rate of dissolution or by the intrinsic rate of biodegradation by the microorganisms.…”

Section: Parameter Estimationmentioning

confidence: 99%

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Moliterni

Jiménez-Tusset

Rayo

et al. 2012

Int. J. Environ. Sci. Technol.

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Three microbial consortia were isolated from three polluted soils located at an oil refinery and acclimated to grow on diesel fuel as the sole carbon source. Batch experiments were then conducted with the three consortia to study the kinetics of diesel biodegradation. The effects of temperature (25, 30 and 35°C) and diesel concentration (0.5, 1 and 3 %) on the biodegradation of diesel were analysed. Several species were identified in the acclimated microbial consortia, and some of them appeared in more than one consortium. Thermal inhibition was observed at 35°C. In the rest of experiments, over 80 % of the substrate was degraded after 40 h of treatment. These results proved the good feasibility of using the polluted sites as sources of mixed consortia for hydrocarbon degradation. However, diesel degradation efficiencies and rates were very similar, suggesting that the acclimation process produced mixed consortia with very similar characteristics; in this context, origin of the soil sample was not a decisive factor. A simple Monod-type kinetic model was used to simulate the biodegradation process, and accurate results were obtained. The l max values were between 0.17 and 0.34 h -1 . The results of this study revealed that the consortia can function at high concentrations of hydrocarbons without any sign of growth inhibition, which is important for the design of bioreactors for wastewater treatment with high concentrations of fuel.

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Biodegradation kinetics of naphthalene in nonaqueous phase liquid-water mixed batch systems: Comparison of model predictions and experimental results

Cited by 23 publications

References 37 publications

Remediation of saturated soil contaminated with petroleum products using air sparging with thermal enhancement

Remediation of saturated soil contaminated with petroleum products using air sparging with thermal enhancement

Products from the Incomplete Metabolism of Pyrene by Polycyclic Aromatic Hydrocarbon-Degrading Bacteria

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

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