Biodegradation model of porous silicon nanoparticles

Gongalsky, M. B.; Sviridov, Andrey; Bezsudnova, Yulia; Осминкина, Л. А.

doi:10.1016/j.colsurfb.2020.110946

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…Therefore, both Raman and PL spectra show that typical dissolution time is about 15–21 days for sol-SiNPs and 24 h for por-Si. The difference is explained by different nc-Si sizes, and also their different specific surface area which is an important factor dissolution rate [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…Biodegradability is based on dissolution of SiNPs in conditions corresponding to living systems. Though dissolution is rather slow, the enormously large specific surface area of SiNPs (especially por-SiNPs) facilitates typical lifetimes in the range from several hours to several days [ 13 , 14 ]. The product of the dissolution is silicic acid (SiOH 4 ), which is non-toxic in relevant concentrations and is easily excreted from organism.…”

Section: Introductionmentioning

confidence: 99%

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Optical Monitoring of the Biodegradation of Porous and Solid Silicon Nanoparticles

et al. 2021

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Silicon nanoparticles (SiNP) are currently of great interest, especially in biomedicine, because of their unique physicochemical properties combined with biodegradability. SiNPs can be obtained in various ways and can have either a non-porous solid (sol-) or porous (por-) structure. In this work, we carry out detailed optical monitoring of sol- and por-SiNP biodegradation using Raman and photoluminescence (PL) micro-spectroscopy. SiNPs were obtained by ultrasound grinding of sol- or por-silicon nanowires, created by silver-assisted chemical etching of crystalline Si with different doping levels. In this case, sol-SiNPs consist of nanocrystals 30 nm in size, while por-SiNPs consist of small 3 nm nanocrystals and 16 nm pores. Both SiNPs show low in vitro cytotoxicity towards MCF-7 and HEK293T cells up to 800 μg/mL. The appearance of the F-band (blue–yellow) PL, as well as a decrease in the intensity of the Raman signal, indicate the gradual dissolution of the sol-SiNPs during 20 days of incubation. At the same time, the rapid dissolution of por-SiNP within 24 h is identified by the quenching of their S-band (red) PL and the disappearance of the Raman signal. The obtained results are important for development of intelligent biodegradable drug delivery systems based on SiNPs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical Monitoring of the Biodegradation of Porous and Solid Silicon Nanoparticles

et al. 2021

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“…Silicon itself was not considered a “biomaterial”. The continuing investigations into nanoporous-silicon bioactivity [ 66 , 67 , 68 , 69 , 70 , 71 ] and biodegradability [ 66 , 72 , 73 , 74 ] are allowing nanoporous-silicon researchers to engage with the biomaterial science and medical communities. Students of this material thus have the opportunity to learn about multiple scientific disciplines via “nanomedicine” [ 75 ]: biomaterials science [ 76 ] as well as the bench to bedside biocompatibility testing process: in vitro cell culture, in vivo animal models, and clinical trials under regulatory processes [ 77 ].…”

Section: Porous Silicon Properties—the Need For Novel Functionalitmentioning

confidence: 99%

Nanoporous Silicon as a Green, High-Tech Educational Tool

Coffer

Canham

2021

Nanomaterials

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Pedagogical tools are needed that link multidisciplinary nanoscience and technology (NST) to multiple state-of-the-art applications, including those requiring new fabrication routes relying on green synthesis. These can both educate and motivate the next generation of entrepreneurial NST scientists to create innovative products whilst protecting the environment and resources. Nanoporous silicon shows promise as such a tool as it can be fabricated from plants and waste materials, but also embodies many key educational concepts and key industrial uses identified for NST. Specific mechanical, thermal, and optical properties become highly tunable through nanoporosity. We also describe exceptional properties for nanostructured silicon like medical biodegradability and efficient light emission that open up new functionality for this semiconductor. Examples of prior lecture courses and potential laboratory projects are provided, based on the author’s experiences in academic chemistry and physics departments in the USA and UK, together with industrial R&D in the medical, food, and consumer-care sectors. Nanoporous silicon-based lessons that engage students in the basics of entrepreneurship can also readily be identified, including idea generation, intellectual property, and clinical translation of nanomaterial products.

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“…Among the available nanomaterials, porous silicon nanoparticles (pSiNPs) belong to promising delivery vehicles due to their high drug-loading capacity, biocompatibility, and biodegradability ( Fornaguera and García-Celma, 2017 ; Wan et al, 2018 ). The pSiNPs are degraded to non-toxic and well-tolerated silicic acid species ( Anderson et al, 2003 ; Park et al, 2009 ; Gu et al, 2013 ; Gongalsky et al, 2020 ). Moreover, the morphology and porosity of pSiNPs can easily be adapted by modification of certain fabrication parameters ( Herino et al, 1987 ; Lehmann et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Raman and fluorescence micro-spectroscopy applied for the monitoring of sunitinib-loaded porous silicon nanocontainers in cardiac cells

et al. 2022

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Nanomaterials are a central pillar in modern medicine. They are thought to optimize drug delivery, enhance therapeutic efficacy, and reduce side-effects. To foster this technology, analytical methods are needed to validate not only the localization and distribution of these nanomaterials, but also their compatibility with cells, drugs, and drug release. In the present work, we assessed nanoparticles based on porous silicon (pSiNPs) loaded with the clinically used tyrosine kinase inhibitor sunitinib for their effectiveness of drug delivery, release, and toxicity in colon cancer cells (HCT 116 cells) and cardiac myoblast cells (H9c2) using Raman micro-spectroscopy, high-resolution fluorescence microscopy, along with biological methods for toxicological effects. We produced pSiNPs with a size of about 100 nm by grinding mesoporous silicon layers. pSiNPs allowed an effective loading of sunitinib due to their high porosity. Photoluminescence properties of the nanoparticles within the visible spectrum allowed the visualization of their uptake in cardiac cells. Raman micro-spectroscopy allowed not only the detection of the uptake and distribution of pSiNPs within the cells via a characteristic silicon Raman band at about 518–520 cm−1, but also the localization of the drug based on its characteristic molecular fingerprints. Cytotoxicity studies by Western blot analyses of apoptotic marker proteins such as caspase-3, and the detection of apoptosis by subG1-positive cell fractions in HCT 116 and MTT analyses in H9c2 cells, suggest a sustained release of sunitinib from pSiNPs and delayed cytotoxicity of sunitinib in HCT 116 cells. The analyses in cardiac cells revealed that pSiNPs are well tolerated and that they may even protect from toxic effects in these cells to some extent. Analyses of the integrity of mitochondrial networks as an early indicator for apoptotic cellular effects seem to validate these observations. Our study suggests pSiNPs-based nanocontainers for efficient and safe drug delivery and Raman micro-spectroscopy as a reliable method for their detection and monitoring. Thus, the herein presented nanocontainers and analytical methods have the potential to allow an efficient advancement of nanoparticles for targeted and sustained intracellular drug release that is of need, e.g., in chronic diseases and for the prevention of cardiac toxicity.

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Biodegradation model of porous silicon nanoparticles

Cited by 21 publications

References 33 publications

Optical Monitoring of the Biodegradation of Porous and Solid Silicon Nanoparticles

Optical Monitoring of the Biodegradation of Porous and Solid Silicon Nanoparticles

Nanoporous Silicon as a Green, High-Tech Educational Tool

Raman and fluorescence micro-spectroscopy applied for the monitoring of sunitinib-loaded porous silicon nanocontainers in cardiac cells

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