Biodegradation of 3-Nitrobenzoate by Bacillus flexus strain XJU-4

Mulla, Sikandar I.; Manjunatha, T. P.; Hoskeri, Robertcyril S.; Tallur, Preeti N.; Ninnekar, Harichandra Z.

doi:10.1007/s11274-010-0611-4

Cited by 23 publications

(10 citation statements)

References 52 publications

(34 reference statements)

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“…The by products were observed at 254 nm under ultraviolet (UV) chamber and/or by exposing iodine vapours on TLC glass plates in TLC chamber. Phenolic compounds were confirmed by their characteristic colour on spraying with 2% solution of 2,6dichloroquinone-4-chlorimide in methanol (Gibbs reagent) or using diazotized p-nitroaniline (Mulla et al 2011b). ortho-Dihydroxy compounds were confirmed by spraying with Arnow's reagent (1937).…”

Section: Methodsmentioning

confidence: 99%

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Aerobic degradation of fenvalerate by a Gram-positive bacterium, Bacillus flexus strain XJU-4

Mulla

Ameen

Tallur³

et al. 2017

3 Biotech

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Synthetic pyrethroid-fenvalerate-is one of the most widespread toxic pollutants and has adverse effect on living systems. However, little is known about its biotransformation mechanism in different microorganisms. To elucidate the pathway that might be involved in the catabolism of fenvalerate, we used strain XJU-4 (3-nitrobenzoate degrading organism) as an ideal fenvalerate degrading bacterium. Thin layer chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry analysis results revealed that 3-phenoxybenzoate, protocatechuate, and catechol are the three main by-products of fenvalerate metabolism. Additionally, the bacterial cell-free enzymes showed the activities of fenvalerate hydrolyzing esterase, 3-phenoxybenzaldehyde dehydrogenase, 3-phenoxybenzoate dioxygenase, phenol hydroxylase, protocatechuate 2,3-dioxygenase and catechol-2,3-dioxygenase. Thus, in strain XJU-4, protocatechuate and catechol were further metabolized through-cleavage pathway. Moreover, laboratory-scale soil experiments results suggest that strain XJU-4 is a suitable contender for bioremediation of pyrethroid fenvalerate-contaminated sites.

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Section: Methodsmentioning

confidence: 99%

“…ortho-Dihydroxy compounds were confirmed by spraying with Arnow's reagent (1937). GC-MS analysis was performed using a Shimadzu QP2010 Plus (Tokyo, Japan) as described previously (Mulla et al 2011b). The by-products were analyzed by HPLC (Shimadzu, Kyoto, Japan) equipped with SPD-10AVP UV-Detector.…”

Section: Methodsmentioning

confidence: 99%

Aerobic degradation of fenvalerate by a Gram-positive bacterium, Bacillus flexus strain XJU-4

Mulla

Ameen

Tallur³

et al. 2017

3 Biotech

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“…Bacillus oleronius was first isolated from the hindgut of the termite Reticulitermes santonensis (Kuhnigk et al ., ) and has been reported to be closely related to some Bacillus species. B. flexus is also gram‐positive, spore‐forming, aerobic and tile rod alkaliphilic bacteria and always isolated from contaminated soil and waste water (Mulla et al ., ; Sanchez‐Gonzalez et al ., ). This study is the first to report the isolation of Bacillus strains from bottled soy sauce.…”

Section: Discussionmentioning

confidence: 99%

Comparison of microbial communities between normal and swollen canned soy sauces using nested PCR‐denaturing gradient gel electrophoresis, HPLC and plate techniques

Cheng

Lin

Pan

et al. 2014

Int J of Food Sci Tech

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Summary The microbial community of normal and swollen canned soy sauce was investigated using molecular biological method. The PCR‐denaturing gradient gel electrophoresis (DGGE) profiles showed that four lactic acid bacteria (LAB), including Lactobacillus acidipiscis, L. pobuzihii, L. piscium and another Lactobacillus sp., were involved in the swollen canned samples. Plate technique showed that three diverse species of Bacillus (B. subtilis, B. oleronius and B. flexus) were present in the swollen canned samples. However, much less bacterial contaminants were detected in the normal samples. According to the HPLC analysis, the lactic acid concentrations of the swollen canned samples were significantly higher than those in the normal samples. These results indicated that LAB can play a key role in contributing to the acidisation of the swollen canned soy sauce products. Our results confirmed the existence of Bacillus sp. and LAB in the packaged fermented soy sauce.

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“…The cell-free extract were prepared through ultrasonication (Ultrasonic homogenizer JY92-IIN, Ningbo Scientz Biotechnology Co., Ltd., Ningbo, China) followed by centrifugation (12,500 × g for 40 min at 4 °C). The supernatant obtained was used as the crude enzyme for enzyme assays 40 . Chlorohydroquinone dehalogenase activity was assayed according to the previous literature 35 .…”

Section: Methodsmentioning

confidence: 99%

Characterization of triclosan metabolism in Sphingomonas sp. strain YL-JM2C

Mulla

Wang

Sun

et al. 2016

Sci Rep

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Triclosan (TCS) is one of the most widespread emerging contaminants and has adverse impact on aquatic ecosystem, yet little is known about its complete biodegradation mechanism in bacteria. Sphingomonas sp, strain YL-JM2C, isolated from activated sludge of a wastewater treatment plant, was very effective on degrading TCS. Response surface methodology (RSM) was applied to optimize the conditions like temperature and pH. From RSM, the optimal TCS degradation conditions were found to be 30 °C and pH 7.0. Under optimal conditions, strain YL-JM2C completely mineralized TCS (5 mg L−1) within 72 h. Gas chromatography-mass spectrometry analysis revealed that 2,4-dichlorophenol, 2-chlorohydroquinone and hydroquinone are three main by-products of TCS. Furthermore, stable isotope experimental results revealed that the 13C12-TCS was completely mineralized into CO2 and part of heavier carbon (13C) of labeled TCS was utilized by strain YL-JM2C to synthesize fatty acids (PLFAs). Cell surface hydrophobicity (CSH) and degradation test results suggested that the strain could enhance degradation capacity of TCS through increasing CSH. In addition, the bacterium also completely degraded spiked TCS (5 mg L−1) in wastewater collected from the wastewater treatment plant. Hence, these results suggest that the strain has potential to remediate TCS in the environment.

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Biodegradation of 3-Nitrobenzoate by Bacillus flexus strain XJU-4

Cited by 23 publications

References 52 publications

Aerobic degradation of fenvalerate by a Gram-positive bacterium, Bacillus flexus strain XJU-4

Aerobic degradation of fenvalerate by a Gram-positive bacterium, Bacillus flexus strain XJU-4

Comparison of microbial communities between normal and swollen canned soy sauces using nested PCR‐denaturing gradient gel electrophoresis, HPLC and plate techniques

Characterization of triclosan metabolism in Sphingomonas sp. strain YL-JM2C

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