Biodegradation of Amphipathic Fluorinated Peptides Reveals a New Bacterial Defluorinating Activity and a New Source of Natural Organofluorine Compounds

Khan, Mohd Faheem; Chowdhary, Suvrat; Koksch, Beate; Murphy, Cormac D.

doi:10.1021/acs.est.3c01240

Cited by 4 publications

(1 citation statement)

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“…The ecological value of soil bacteria is well reflected by their role in element cycling, such as carbon, nitrogen, metals or phosphorous, and in the decomposition of dead plant biomass [ 17 ], through which their existence and functioning are crucial in the life of forests. The role of soil bacteria in the biodegradation of pollutants is also prominent [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests

Bereczki,

Tóth,

Szili-Kovács

et al. 2024

Microorganisms

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Soil bacterial communities play a remarkable role in nutrient cycling, significantly affecting soil organic material content, soil fertility, and, in an indirect way, plant succession processes. Conversely, vegetation type influences microbial soil life. The present study compared the bacterial microbiome composition, diversity and catabolic activity profile of topsoil samples collected under three different forest types (a twice-coppiced black locust stand, a young, naturally reforested, and a middle-aged mixed pedunculate oak stand) planted on former arable land in the early 20th century. Diversity indices determined during 16S ribosomal RNA sequencing-based metagenome analysis indicated that the black locust stand had the highest soil bacterial community diversity. At the phylum level, Acidobacteriota, Actinobacteriota, Proteobacteria, Verrucomicrobiota, Bacteroidota, and Gemmatimonadota were the most abundant taxa in the forest soils. Concerning soil parameters, redundancy analysis revealed that pH had the highest impact on bacterial community structure and pH, and soil organic carbon content on the samples’ respiration patterns. As for catabolic activity, the recently clearcut oak forest showed the lowest substrate-induced respiration, and citrate was the main driver for the inter-stand variability of microbial activity. Our results confirm that soil parameters and forest type influence the composition and functioning of the soil bacterial microbiome.

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Section: Introductionmentioning

confidence: 99%

Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests

Bereczki,

Tóth,

Szili-Kovács

et al. 2024

Microorganisms

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Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil

Yan,

Gao,

Xiang

et al. 2024

AMB Expr

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Microbial degradation of fluorinated compounds raised significant attention because of their widespread distribution and potential environmental impacts. Here, we report a bacterial isolate, Rhodococcus sp. NJF-7 capable of defluorinating monofluorinated medium-chain length alkanes. This isolate consumed 2.29 ± 0.13 mmol L− 1 of 1-fluorodecane (FD) during a 52 h incubation period, resulting in a significant release of inorganic fluoride amounting to 2.16 ± 0.03 mmol L− 1. The defluorination process was strongly affected by the initial FD concentration and pH conditions, with lower pH increasing fluoride toxicity to bacterial cells and inhibiting enzymatic defluorination activity. Stoichiometric conversion of FD to fluoride was observed at neutral pH with resting cells, while defluorination was significantly lower at reduced pH (6.5). The discovery of the metabolites decanoic acid and methyl decanoate suggests that the initial attack by monooxygenases may be responsible for the biological defluorination of FD. The findings here provide new insights into microbial defluorination processes, specifically aiding in understanding the environmental fate of organic semi-fluorinated alkane chemicals.

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Leguminous Green Manure Intercropping Promotes Soil Health in a Citrus (Citrus reticulata) Orchard

Xie,

Jing,

Wang

et al. 2024

Agriculture

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The intercropping of green manure is an important and sustainable production method in citrus orchards (Citrus reticulata). However, few studies focus on the impact of annual and perennial green manure on soil health, particularly soil microbiome and properties in acid soil. Our research objective was to explore the potential effects on soil health by intercropping with annual and perennial leguminous green manures in acid soil citrus orchards of southwestern China. The leguminous green manures used were alfalfa (Medicago sativa) and hairy vetch (Vicia villosa). The results showed that intercropping with green manure increased the total nitrogen, nitrate nitrogen, and available phosphorous in the soil by 48.67~74.67%, 50.00~96.67%, and 44.48~45.04%, respectively. Intercropping with alfalfa significantly increased the activity of soil sucrase 63.75%, and intercropping with hairy vetch increased the activity of β-1,4-glucosidase 44.38% in the soil compared to the monoculture treatment. Intercropping hairy vetch and alfalfa altered the diversity and composition of the soil microbial community and enriched the soil with beneficial fungi and bacteria, including Mortierella and Streptomyces. The richness increased by 58.72% and 17.90% in alfalfa intercropping treatment. In conclusion, intercropping leguminous green manure improved the nutrients and activity of the enzymes in the soil and enriched the antagonistic microbiome in the soil, promoting soil health in the citrus orchard.

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Biodegradation of Amphipathic Fluorinated Peptides Reveals a New Bacterial Defluorinating Activity and a New Source of Natural Organofluorine Compounds

Cited by 4 publications

References 50 publications

Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests

Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests

Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil

Leguminous Green Manure Intercropping Promotes Soil Health in a Citrus (Citrus reticulata) Orchard

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