Biodegradation of Azure-B dye by Serratia liquefaciens and its validation by phytotoxicity, genotoxicity and cytotoxicity studies

Haq, Izharul; Raj, Abhay; Markandeya,

doi:10.1016/j.chemosphere.2017.12.153

Cited by 123 publications

(28 citation statements)

References 43 publications

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“…The phytotoxicity of the dyes was examined using the seed germination/root elongation toxicity test with cucumber (C. sativus), lettuce (L. sativa) and tomato (L. esculentum) seeds. The plant seed germination and early seedling growth test has been considered as one of the simplest and short-term method for the study of the general toxicity of chemicals and industrial wastewaters [51]. DB38 and RB15 had no significant effect on seed germination ( Fig.…”

Section: Resultsmentioning

confidence: 99%

A test battery for assessing the ecotoxic effects of textile dyes

Oliveira

Leme

Lapuente

et al. 2018

Chemico-Biological Interactions

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The textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2-50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was used to assess the acute toxicity and genotoxicity of the textile dyes Direct Black 38 (DB38; azo dye) and Reactive Blue 15 (RB15; copper phthalocyanine dye) on different trophic levels. Thus these dyes were tested using the following assays: Filter paper contact test with earthworms (Eisenia foetida); seed germination and root elongation toxicity test (Cucumis sativus, Lactuca sativa and Lycopersicon esculentum); acute immobilization test (Daphnia magna and Artemia salina); and the Comet assay with the rainbow trout gonad-2 cell fish line (RTG-2) and D. magna. Neither phytotoxicity nor significant effects on the survival of E. foetida were observed after exposure to DB38 and RB15. Both dyes were classified as relatively non-toxic to D. magna (LC > 100 mg/L), but DB38 was moderately toxic to A. salina with a LC of 20.7 mg/L. DB38 and RB15 induced significant effects on the DNA of D. magna but only DB38 caused direct (alkaline comet assay) and oxidative (hOGG1-modified alkaline comet assay) damage to RTG-2 cells in hormetic responses. Therefore, the present results emphasize that a test battery approach of bioassays representing multiple trophic levels is fundamental in predicting the toxicity of textile dyes, aside from providing the information required to define their safe levels for living organisms in the environment.

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Section: Resultsmentioning

confidence: 99%

A test battery for assessing the ecotoxic effects of textile dyes

Oliveira

Leme

Lapuente

et al. 2018

Chemico-Biological Interactions

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“…Out of these 86 isolates, 62 various bacterial strains showed dye decolorization ability on BH medium supplemented with variable concentrations of different dyes (congo red, methyl orange, and reactive red 198). The presence of microorganisms in the textile dye effluents indicates their natural adaptation and survival abilities in the presence of toxic dyes (Haq et al, 2018). On BH plates, all 62 isolates generated decolorization zones around their colonies but only six isolates had maximum zones of dye colour removal in the plate assay (from 40 to 56 mm).…”

Section: Isolation and Screening Of Bacterial Isolates For Dye Decolomentioning

confidence: 99%

Textile azo dye decolorization and detoxification using bacteria isolated from textile effluents

Karnwal¹

2019

bta

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Azo dyes, which are highly toxic, carcinogenic, and mutagenic for living organisms, are used as coloring chemicals in textile industries. Physicochemical methods used for removing azo dyes are expensive, can generate secondary waste, and are not very efficient. In this study, we used a biological approach to reduce the toxicity of three azo dyes, i.e., congo red, methyl orange, and reactive red 198, from textile effluents. Six dye-decolorizing bacteria were screened from waste water obtained from the textile industry (in the dyeing process) at a concentration 100 mg/l for each azo dye. Using a 16S rRNA approach, the bacteria were identified and assigned as Enterococcus faecalis VTK04, Staphylococcus aureus VTK013, Pseudomonas aeruginosa VTK018, Proteus mirabilis VTK024, Bacillus cereus VTK035, and Enterococcus faecium VTK054. These bacteria were tested for their ability to produce biofilms on the abiotic surface. The adherence assay showed that VTK013, VTK054, and VTK024 had a potential to form stable biofilms on abiotic surfaces (OD570 = 1.37). Moreover, the dye decolorization potential was spectrophotometrically measured after seven days with and without a carbon substrate. The results demonstrated that the absence of the carbon source had a negative impact on decolorization, whereas a carbon-supplemented medium showed a considerable increase in the decolorization of congo red (80% and 96% by VTK013 and VTK018, respectively), methyl orange (100% and 75% by VTK054 and VTK035, respectively), and reactive red 198 (90% and 86% by VTK04 and VTK024, respectively). The phytotoxicity study of the treated dye solutions showed lesser toxicity compared to the untreated dye solution. These results support the possibility of using bacterial isolates for the biodegradation of azo dye effluents.

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“…The absence of mutagenic effect of azo dye metabolite samples from aerobic cultures with probiotic could be clarified by the presence of an adequate level of dissolved oxygen that might limit the azo-reductase action and raise the oxygenase activity; thus granting the decolorization of the dye aromatic amines to which the mutagenic effects may be assigned. Marques et al [45], Nakayama et al [46], Sweeney et al [47], Erdoğdular and Dilek [48] and Jayalakshmi and Jeyanthi [49] reported that carcinogenicity via mutagenicity of some azo dyes and allied aromatic amines could be engendered Fig. 7.…”

Section: Mutagenicity Assessmentmentioning

confidence: 99%

Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization

Ayed¹,

Zmantar²,

Bayar³

et al. 2019

Journal of Microbiology and Biotechnology

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Azo dyes are recalcitrant pollutants, which are toxic, carcinogenic, mutagenic and teratogenic, that constitute a significant burden to the environment. The decolorization and the mineralization efficiency of Remazol Brillant Orange 3R (RBO 3R) was studied using a probiotic consortium (Lactobacillus acidophilus and Lactobacillus plantarum). Biodegradation of RBO 3R (750 ppm) was investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 11.5 and temperature 25 o C. The bio-decolorization process was further confirmed by FTIR and UV-Vis analysis. Under optimal conditions, the bacterial consortium was able to decolorize the dye completely (>99%) within 12 h. The color removal was 99.37% at 750 ppm. Muliplex PCR technique was used to detect the Lactobacillus genes. Using phytotoxicity, cytotoxicity, mutagenicity and biototoxicity endpoints, toxicological studies of RBO 3R before and after biodegradation were examined. A toxicity assay signaled that biodegradation led to detoxification of RBO 3R dye.

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Biodegradation of Azure-B dye by Serratia liquefaciens and its validation by phytotoxicity, genotoxicity and cytotoxicity studies

Cited by 123 publications

References 43 publications

A test battery for assessing the ecotoxic effects of textile dyes

A test battery for assessing the ecotoxic effects of textile dyes

Textile azo dye decolorization and detoxification using bacteria isolated from textile effluents

Potential Use of Probiotic Consortium Isolated from Kefir for Textile Azo Dye Decolorization

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