Biodegradation of benzyl benzoate by Pseudomonas desmolyticum NCIM 2112

Rokade, Kedar; Mali, G. V.

doi:10.1007/s11274-013-1484-0

Cited by 12 publications

(16 citation statements)

References 12 publications

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“…Therefore, bacterial community had functional groups which could metabolites polysaccharides resulting simpler sugars to degrade chlorpyrifos. Similar result has been observed where simple glucose and maltose promoted chlorpyrifos degradation and produced non toxic compound like 2-pyridinol and thiophosphate [27]. Based in the C source utilization pattern it is plausible that the biodegradation of chlorpyrifos occurred by group of bacteria having different metabolic attributes, by the bacterial groups which use complex C sources (polysaccharides) and then by the bacterial groups which preferentially use simpler C sources (monosaccharide or disaccharide).…”

Section: Discussionsupporting

confidence: 69%

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

et al. 2019

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It is predicted that use of insecticide chlorpyrifos will increase in near future due to intensive agricultural practice and climate change. The present study envisages establishing linkages among biodegradation potential, carbon metabolism and enzymatic attributes of bacteria involved in chlorpyrifos biodegradation. Soil samples from an experimental field were incubated with chlorpyrifos (10 µg g −1 soil). Chlorpyrifos biodegradation rate was 0.34 ± 0.04 ug g −1 soil d −1 . Culturable bacteria were isolated and identified by 16S rRNA gene sequencing. DNA sequences were 96-100% similar to the GenBank bacterial DNA sequences. The bacterial community was predominated by Arthrobacter phenanthrenivorans (23%). Followed by Arthrobacter globiformis and Bacillus flexus represented 15% each. Microbacterium paraoxydans, Bacillus megaterium, Bacillus soli strain R, Bacillus drentensis, and Bacillus koreensis represented 8% of total bacteria. The carbon source utilization pattern and enzymatic activities of the bacterial isolates were estimated. The isolates preferred to metabolize trehalose and mannose over other C sources. These bacteria exhibited decarboxylase, oxidase, catalase, cellulose, deaminase and nitrate reductase activities. A PCA analysis based on various metabolic attributes explained 32.49% variance by PC1 and 16.46% variance by PC2. PCA highlights that the bacterial isolates placed distantly from those of simple sugar utilizers, probably use chlorpyrifos as C substrate. Correspondence analysis outlaid decarboxylase, catalase, and oxidase as the key enzymatic activities of the bacterial isolates for chlorpyrifos biodegradation.

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Section: Discussionsupporting

confidence: 69%

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

et al. 2019

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“…The significance of biotic degradation of soil-incorporated chlorpyrifos was indicated by a large difference in degradation rates of pesticide in unsterilized and sterilized soils, i.e., 6 × 10 −2 day −1 and t 0.5 of 25 days ( Fig. 1 and Table 2), thus evidencing the significant role of microbes in dissipating and detoxifying the residues of chlorpyrifos in the soil (Jabeen et al 2014;Rokade and Mali 2013). Chlorpyrifos also inhibits the growth of bacterial populations present in the soil and interrupts the degradation of soil organic matter.…”

Section: Photodegradation Studies Of Soil-incorporated Chlorpyrifosmentioning

confidence: 96%

Cu2+ and Fe2+ mediated photodegradation studies of soil-incorporated chlorpyrifos

Rafique

Tariq

Ahad

et al. 2015

Environ Sci Pollut Res

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The influences of Cu(2+) and Fe(2+) on the photodegradation of soil-incorporated chlorpyrifos were investigated in the present study. The soil samples spiked with chlorpyrifos and selected metal ions were irradiated with UV light for different intervals of time and analyzed by HPLC. The unsterile and sterile control soil samples amended with pesticides and selected metals were incubated in the dark at 25 °C for the same time intervals. The results of the study evidenced that photodegradation of chlorpyrifos followed the first-order kinetics. The dissipation t0.5 of chlorpyrifos was found to decrease from 41 to 20 days under UV irradiation. The rate of chlorpyrifos photodegradation was increased in the presence of both metals, i.e., Cu(2+) and Fe(2+). Thus, initially observed t0.5 of 19.8 days was decreased to 4.39 days in the case of Cu(+2) and 19.25 days for Fe(+2). Copper was found to increase the rate of photodegradation by 4.5 orders of magnitude while the microbial degradation of chlorpyrifos was increased only twofold. The microbial degradation of chlorpyrifos was only negligibly affected by Fe(2+) amendment. The studied trace metals also affected the abiotic degradation of the pesticide in the order Cu(2+) > Fe(2+).

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“…Percent degradation of compound was determined by using the formula Percent degradation= Ab-Aa ÷ Ab 100 Where, Ab is absorbance of compound before degradation. Aa is absorbance of compound after degradation (11).…”

Section: Degradation Of Chlorpyrifos:-mentioning

confidence: 99%

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Hamsavathani¹,

Aysha²,

Ajith³

2017

IJAR

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The extensive use of pesticides in agricultural fields for pest control pose a serious problem in contaminating soil and water ecosystems. Chlorpyrifos is the major broad spectrum organophosphorus insecticide used in paddy fields against sucking, chewing, boring insects. Due to its toxicity and persistence in the environment, there is an immediate need to eliminate them from contaminated sites by biodegradation. Two pesticide degrading bacteria were screened and isolated from chlorpyrifos contaminated soil by enrichment culture technique and were identified as Kocuria kristinae and Staphylococcus aureus. The growth response and degradation of chlorpyrifos by the isolates in MSM broth supplemented with 0.5% chlorpyrifos was monitored every 48-72 hrs in spectrophotometer at 600nm. Kocuria sp showed maximum growth in 7 days than Staphylococcus aureus. The degradation efficiency of the strains were determined and estimated by the removal percentage of chlorpyrifos from the liquid culture. Both the isolate showed the degrading capability of chlorpyrifos in MSM. The isolate, S. aureus was more potent in degrading the 80% of the total compound from the media in 2 weeks of incubation than K. kristinae which shows 35 % of degradation. These results were further confirmed by GCMS, in which S. aureus has degraded 82.06% and K. kristinae has degraded 30.78% of chlorpyrifos in the medium. This study indicates that the isolate, Staphylococcus aureus is more potent in degrading chlorpyrifos in liquid culture and can also be used in bioremediation of chlorpyrifos contaminated soils.

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Biodegradation of benzyl benzoate by Pseudomonas desmolyticum NCIM 2112

Cited by 12 publications

References 12 publications

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

Cu2+ and Fe2+ mediated photodegradation studies of soil-incorporated chlorpyrifos

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

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