Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase

Yang, Jia; Eltoukhy, Adel; Wang, Junhuan; Li, Xianjun; Hlaing, Thet Su; Aung, Mar Mar; Nwe, May Thet; Lamraoui, Imane; Yan, Yanchun

doi:10.3390/ijms21103588

Cited by 55 publications

(32 citation statements)

References 46 publications

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“…The biodegradability of BPA has been demonstrated in many species of fungi and bacteria [ 29 , 30 , 31 ]. The elimination of this compound with the participation of laccase produced by white rot fungi is also well characterized [ 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…This mechanism of xenobiotic degradation is common in fungi and involves the transformation of a non-growth substrate in the obligate presence of a growth substrate or another transformable compound [27,28]. The biodegradability of BPA has been demonstrated in many species of fungi and bacteria [29][30][31]. The elimination of this compound with the participation of laccase produced by white rot fungi is also well characterized [32,33].…”

Section: Fungal Growth Bpa Biodegradation and Laccase Involvementmentioning

confidence: 99%

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Bisphenol A Removal by the Fungus Myrothecium roridumIM 6482—Analysis of the Cellular and Subcellular Level

Jasińska

Soboń²,

Różalska

et al. 2021

IJMS

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Bisphenol (BPA) is a key ingredient in the production of epoxy resins and some types of plastics, which can be released into the environment and alter the endocrine systems of wildlife and humans. In this study, the ability of the fungus M. roridumIM 6482 to BPA elimination was investigated. LC-MS/MS analysis showed almost complete removal of BPA from the growth medium within 72 h of culturing. Products of BPA biotransformation were identified, and their estrogenic activity was found to be lower than that of the parent compound. Extracellular laccase activity was identified as the main mechanism of BPA elimination. It was observed that BPA induced oxidative stress in fungal cells manifested as the enhancement in ROS production, membranes permeability and lipids peroxidation. These oxidative stress markers were reduced after BPA biodegradation (72 h of culturing). Intracellular proteome analyses performed using 2-D electrophoresis and MALDI-TOF/TOF technique allowed identifying 69 proteins in a sample obtained from the BPA containing culture. There were mainly structural and regulator proteins but also oxidoreductive and antioxidative agents, such as superoxide dismutase and catalase. The obtained results broaden the knowledge on BPA elimination by microscopic fungi and may contribute to the development of BPA biodegradation methods.

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Section: Resultsmentioning

confidence: 99%

Section: Fungal Growth Bpa Biodegradation and Laccase Involvementmentioning

confidence: 99%

Bisphenol A Removal by the Fungus Myrothecium roridumIM 6482—Analysis of the Cellular and Subcellular Level

Jasińska

Soboń²,

Różalska

et al. 2021

IJMS

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“…Sphingobium sp. were reported to degrade Bisphenol A ( Jia et al, 2020 ), carbofuran ( Jiang et al, 2020 ) and 17 Archetypal Organophosphate ( Azam et al, 2019 ). Novosphingobium sp.…”

Section: Resultsmentioning

confidence: 99%

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Chen

Zhang

et al. 2021

Front. Microbiol.

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Phenol is a toxic organic molecule that is widely detected in the natural environment, even in drinking water sources. Biological methods were considered to be a good tool for phenol removal, especially microbial immobilized technology. However, research on the “seed” bacteria along with microbial community analysis in oligotrophic environment such as drinking water system has not been addressed. In this study, Acinetobacter sp. DW-1 with high phenol degradation ability had been isolated from a drinking water biofilter was used as seeded bacteria to treat phenol micro-polluted drinking water source. Meanwhile, the whole genome of strain DW-1 was sequenced using nanopore technology. The genomic analysis suggests that Acinetobacter sp. DW-1 could utilize phenol via the β-ketoadipate pathway, including the catechol and protocatechuate branches. Subsequently, a bio-enhanced polyhedral hollow polypropylene sphere (BEPHPS) filter was constructed to investigate the stability of the seeded bacteria during the water treatment process. The denatured gradient gel electrophoresis (DGGE) profile and the quantification of phenol hydroxylase gene results indicate that when the BEPHPS filter was operated for 56 days, Acinetobacter sp. was still a persistent and competitive bacterium in the treatment group. In addition, 16S rRNA gene amplicon sequencing results indicate that Acinetobacter sp., as well as Pseudomonas sp., Nitrospira sp., Rubrivivax sp. were the predominant bacteria in the treatment group, which were different from that in the CK group. This study provides a better understanding of the mechanisms of phenol degradation by Acinetobacter sp. DW-1 at the gene level, and provides new insights into the stability of seeded bacteria and its effects on microbial ecology during drinking water treatment.

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“…is strain could utilize 4-hydroxybenzaldehyde and 4′-hydroxyacetophenone as a sole carbon source. Strain YC-JY1ΔbisdB was constructed in E. coli to explore the role of the P450 [45]. Also, Kan et al isolated…”

Section: Some Microbial Enzymesmentioning

confidence: 99%

Microbial Enzymes Used in Bioremediation

Bhandari

Poudel

Marahatha

et al. 2021

Journal of Chemistry

173

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Emerging pollutants in nature are linked to various acute and chronic detriments in biotic components and subsequently deteriorate the ecosystem with serious hazards. Conventional methods for removing pollutants are not efficient; instead, they end up with the formation of secondary pollutants. Significant destructive impacts of pollutants are perinatal disorders, mortality, respiratory disorders, allergy, cancer, cardiovascular and mental disorders, and other harmful effects. The pollutant substrate can recognize different microbial enzymes at optimum conditions (temperature/pH/contact time/concentration) to efficiently transform them into other rather unharmful products. The most representative enzymes involved in bioremediation include cytochrome P450s, laccases, hydrolases, dehalogenases, dehydrogenases, proteases, and lipases, which have shown promising potential degradation of polymers, aromatic hydrocarbons, halogenated compounds, dyes, detergents, agrochemical compounds, etc. Such bioremediation is favored by various mechanisms such as oxidation, reduction, elimination, and ring-opening. The significant degradation of pollutants can be upgraded utilizing genetically engineered microorganisms that produce many recombinant enzymes through eco-friendly new technology. So far, few microbial enzymes have been exploited, and vast microbial diversity is still unexplored. This review would also be useful for further research to enhance the efficiency of degradation of xenobiotic pollutants, including agrochemical, microplastic, polyhalogenated compounds, and other hydrocarbons.

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Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase

Cited by 55 publications

References 46 publications

Bisphenol A Removal by the Fungus Myrothecium roridumIM 6482—Analysis of the Cellular and Subcellular Level

Bisphenol A Removal by the Fungus Myrothecium roridumIM 6482—Analysis of the Cellular and Subcellular Level

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

Microbial Enzymes Used in Bioremediation

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