Biodegradation of BTEX: Optimization through Response Surface Methodology

Abstract

Abstract:Response Surface Methodology (RSM) was used to optimize the biodegradation of Benzene, Toluene, Ethyl benzene and m-, o-, p-Xylene (BTEX) by Pseudomonas putida immobilized in PVA matrices in a speciallydesigned novel Spouted Bed Bioreactor System (SBBS). A mixture of 60 mg L −1 of BTEX compounds in air was contacted with the immobilized bacteria in three spouted bed bioreactors in series. The parameters investigated include the contaminated air flow rate, operating temperature, PVA volume and water pH. Maximum biodegradation efficiency was predicted to occur at an air flow rate of 500 mL min, an operating temperature of 33°C, a PVA volume fraction of 30% and a pH of 8.3. Among the evaluated variables, air flow rate had the most significant effect on the efficiency of BTEX biodegradation. At optimum conditions, the novel bioreactor system was able to achieve maximum removal efficiency of 94, 98, 95 and 98% for Benzene, Toluene, Ethylbenzene and Xylene, respectively.

A Statistical Tool for the Optimization of Parameters for the Degradation of Mono-aromatic Pollutants by A Formulated Microbial Consortium

“…Yield obtained for process optimization as per the trials conducted by the response surface methodology-optimal design software analysis ANOVA test for Response surface methodology-optimal design =0.2601; Adj-R 2 = 0.3251 influencing factors were identified by Plackett-Burman and Response Surface Methodology 24 . Response Surface Methodology was used for the optimization of BTEX25 by Pseudomonas putida and maximum removal efficiency of 94, 98, 95…”