Biodegradation of Buprofezin by <i>Rhodococcus</i> sp. Strain YL-1 Isolated from Rice Field Soil

Li, Chao; Zhang, Ji; Wu, Zhiguo; Cao, Li; Yan, Xin; Li, Shunpeng

doi:10.1021/jf205185n

Cited by 27 publications

(14 citation statements)

References 26 publications

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“…Rice eld soil residing Rhodococcus sp. Strain YL-1, has capability of biodegrading buprofezin into four metabolites: 2 isothiocyanato-2-methyl-propane, 2 tertbutylimino-3-isopropyl-1, 3,5-thiadiazinan-4-one N-tert-butyl thioformimidic acid formylaminomethyl ester, and 2-isothiocyanato-propane, (Li et al,2012).…”

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confidence: 99%

WITHDRAWN: Atropine Reverts the Neurobehavioral Toxicity Elicited by Acute Exposure to Buprofezin in Sprague Dowley Rats

muhammad¹

2021

Preprint

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Buprofezin (BPFN) is a thiadiazine insecticide that inhibits chitin synthesis and the moulting in case of white flies, mealybugs and leaf hoppers. The exposed insects are unable to shed their cuticle and ultimately die as moulting ensue. Neurobehavioral toxic effects elicited by buprofezin remained unclear. Furthermore, the reversal of buprofezin induced neurobehavioral toxicity by atropine was not elaborated. Thus, we explored the neurobehavioral toxic consequences of acute buprofezin exposure in adult male rats and effective reversal of these changes by pretreatment with atropine as an antidote. Acute administration of commercial buprofezin (87.9mg/kg/day through oral gavage with corn oil as vehicle) induce a wide range of neurobehavioral toxicity including damage to pyramidal cells of hippocampal CA1, and CA3,region and behavioral impairments as demonstrated through, loss of motor coordination, locomotor activity, fear loss, hearing, sensorimotor, cognitive and spatial navigation impairment following the exposure .These neurobehavioral toxic effect of acute buprofezin exposure were significantly reversed by the 15 min pre-treatment of atropine antidote before the buprofezin administration. Pre-treated atropine (20mg/kg/day;i.p) attenuates the neurobehavioral toxicity induced by buprofezin in male rats. It was suggested that acute buprofezin exposure elevated the acetylcholine level, by inhibiting the synthesis and release of acetylcholine esterase (AChE) in synapse. But the complete mechanisms are remained to be elucidated

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confidence: 99%

WITHDRAWN: Atropine Reverts the Neurobehavioral Toxicity Elicited by Acute Exposure to Buprofezin in Sprague Dowley Rats

muhammad¹

2021

Preprint

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“…Microorganism-based bioremediation is the most promising and efficient strategy for removal of environmental pollutants compared to the conventional physicochemical methods such as photodecomposition, fenton degradation, ozonation, and adsorption, which are known for high operating cost and low efficiency. − There is a growing body of evidence that xenobiotic compounds can be successfully eliminated by diverse microorganisms belonging to different taxonomic groups. − Recently, Cycoń et al reported that Bacillus sp. TDS-2 isolated from sandy soil successfully removed thiophanate-methyl (100 mg kg –1 ) from soils within 24 days.…”

Section: Introductionmentioning

confidence: 99%

Fenpropathrin Biodegradation Pathway in Bacillus sp. DG-02 and Its Potential for Bioremediation of Pyrethroid-Contaminated Soils

Chen

Chang

Deng

et al. 2014

J. Agric. Food Chem.

115

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The widely used insecticide fenpropathrin in agriculture has become a public concern because of its heavy environmental contamination and toxic effects on mammals, yet little is known about the kinetic and metabolic behaviors of this pesticide. This study reports the degradation kinetics and metabolic pathway of fenpropathrin in Bacillus sp. DG-02, previously isolated from the pyrethroid-manufacturing wastewater treatment system. Up to 93.3% of 50 mg L(-1) fenpropathrin was degraded by Bacillus sp. DG-02 within 72 h, and the degradation rate parameters qmax, Ks, and Ki were determined to be 0.05 h(-1), 9.0 mg L(-1), and 694.8 mg L(-1), respectively. Analysis of the degradation products by gas chromatography-mass spectrometry led to identification of seven metabolites of fenpropathrin, which suggest that fenpropathrin could be degraded first by cleavage of its carboxylester linkage and diaryl bond, followed by degradation of the aromatic ring and subsequent metabolism. In addition to degradation of fenpropathrin, this strain was also found to be capable of degrading a wide range of synthetic pyrethroids including deltamethrin, λ-cyhalothrin, β-cypermethrin, β-cyfluthrin, bifenthrin, and permethrin, which are also widely used insecticides with environmental contamination problems with the degradation process following the first-order kinetic model. Bioaugmentation of fenpropathrin-contaminated soils with strain DG-02 significantly enhanced the disappearance rate of fenpropathrin, and its half-life was sharply reduced in the soils. Taken together, these results depict the biodegradation mechanisms of fenpropathrin and also highlight the promising potentials of Bacillus sp. DG-02 in bioremediation of pyrethroid-contaminated soils.

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“…Previous work showed that the inoculation of soil with strain YL-1 significantly accelerated the degradation of buprofezin in the soil (21), implying the potential use of this strain in bioremediation. However, the buprofezin-degrading phenotype of strain YL-1 is unstable when it grows in nutrient-rich medium.…”

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confidence: 94%

“…strain DFS35-4 (20) were able to cometabolize buprofezin, while Rhodococcus sp. strain YL-1 (21) and Bacillus sp. strain BF5 (22) could use buprofezin as their sole sources of carbon and energy for growth.…”

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confidence: 99%

Molecular Mechanism and Genetic Determinants of Buprofezin Degradation

Zhao

Qiu

et al. 2017

Appl Environ Microbiol

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Buprofezin is a widely used insect growth regulator whose residue has been frequently detected in the environment, posing a threat to aquatic organisms and nontarget insects. Microorganisms play an important role in the degradation of buprofezin in the natural environment. However, the relevant catabolic pathway has not been fully characterized, and the molecular mechanism of catabolism is still completely unknown. Rhodococcus qingshengii YL-1 can utilize buprofezin as a sole source of carbon and energy for growth. In this study, the upstream catabolic pathway in strain YL-1 was identified using tandem mass spectrometry. Buprofezin is composed of a benzene ring and a heterocyclic ring. The degradation is initiated by the dihydroxylation of the benzene ring and continues via dehydrogenation, aromatic ring cleavage, breaking of an amide bond, and the release of the heterocyclic ring 2-tert-butylimino-3-isopropyl-1,3,5-thiadiazinan-4-one (2-BI). A buprofezin degradation-deficient mutant strain YL-0 was isolated. A comparative genomic analysis combined with gene deletion and complementation experiments revealed that the gene cluster bfzBA3A4A1A2C is responsible for the upstream catabolic pathway of buprofezin. The bfzA3A4A1A2 cluster encodes a novel Rieske nonheme iron oxygenase (RHO) system that is responsible for the dihydroxylation of buprofezin at the benzene ring; bfzB is involved in dehydrogenation, and bfzC is in charge of benzene ring cleavage. Furthermore, the products of bfzBA3A4A1A2C can also catalyze dihydroxylation, dehydrogenation, and aromatic ring cleavage of biphenyl, flavanone, flavone, and bifenthrin. In addition, a transcriptional study revealed that bfzBA3A4A1A2C is organized in one transcriptional unit that is constitutively expressed in strain YL-1. IMPORTANCEThere is an increasing concern about the residue and environmental fate of buprofezin. Microbial metabolism is an important mechanism responsible for the buprofezin degradation in the natural environment. However, the molecular mechanism and genetic determinants of microbial degradation of buprofezin have not been well identified. This work revealed that gene cluster bfzBA3A4A1A2C is responsible for the upstream catabolic pathway of buprofezin in Rhodococcus qingshengii YL-1. The products of bfzBA3A4A1A2C could also degrade bifenthrin, a widely used pyrethroid insecticide. These findings enhance our understanding of the microbial degradation mechanism of buprofezin and benefit the application of strain YL-1 and bfzBA3A4A1A2C in the bioremediation of buprofezin contamination.

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Biodegradation of Buprofezin by Rhodococcus sp. Strain YL-1 Isolated from Rice Field Soil

Cited by 27 publications

References 26 publications

WITHDRAWN: Atropine Reverts the Neurobehavioral Toxicity Elicited by Acute Exposure to Buprofezin in Sprague Dowley Rats

WITHDRAWN: Atropine Reverts the Neurobehavioral Toxicity Elicited by Acute Exposure to Buprofezin in Sprague Dowley Rats

Fenpropathrin Biodegradation Pathway in Bacillus sp. DG-02 and Its Potential for Bioremediation of Pyrethroid-Contaminated Soils

Molecular Mechanism and Genetic Determinants of Buprofezin Degradation

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