Biodegradation of chlorpyrifos by charcoal–alginate immobilized bacterial consortium isolated from pesticide-polluted Kuzhikandam Creek, Kerala, India

Raj, Asha S.; Anup, Gadha; Sivalingam, Rajathy; Chandran, Preethy

doi:10.1080/10889868.2024.2312463

Cited by 1 publication

(1 citation statement)

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“…This strain can be isolated from soil or marine environments and is commonly found in the environment [48,50]. Previous research has extensively used alginate-Ca [51,52] and WPU [53,54] in immobilized bacterial biodegradation. However, the innovative microbial immobilization method reported here, involving adsorption of bacteria into pores on biochar surfaces followed by sequential encapsulation with alginate-Ca and WPU has not been previously reported.…”

Section: Discussionmentioning

confidence: 99%

Innovative Microbial Immobilization Strategy for Di-n-Butyl Phthalate Biodegradation Using Biochar-Calcium Alginate-Waterborne Polyurethane Composites

Cao,

Jien,

Yang

et al. 2024

Microorganisms

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Di-n-butyl phthalate (DBP) is a prevalent phthalate ester widely used as a plasticizer, leading to its widespread presence in various environmental matrices. This study presents an innovative microbial immobilization strategy utilizing biochar, calcium alginate (alginate-Ca, (C12H14CaO12)n), and waterborne polyurethane (WPU) composites to enhance the biodegradation efficiency of DBP. The results revealed that rice husk biochar, pyrolyzed at 300 °C, exhibits relatively safer and more stable physical and chemical properties, making it an effective immobilization matrix. Additionally, the optimal cultural conditions for Bacillus aquimaris in DBP biodegradation were identified as incubation at 30 °C and pH 7, with the supplementation of 0.15 g of yeast extract, 0.0625 g of glucose, and 1 CMC of Triton X-100. Algal biotoxicity results indicated a significant decrease in biotoxicity, as evidenced by an increase in chlorophyll a content in Chlorella vulgaris following DBP removal from the culture medium. Finally, microbial community analysis demonstrated that encapsulating B. aquimaris within alginate-Ca and WPU layers not only enhanced DBP degradation, but also prevented ecological competition from indigenous microorganisms. This novel approach showcases the potential of agricultural waste utilization and microbial immobilization techniques for the remediation of DBP-contaminated environments.

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