Biodegradation of chlorpyrifos by soil bacteria and their effects on growth of rice seedlings under pesticide-contaminated soil

Abstract

This study identified three soil bacteria (NRRU-BW3, NRRU-BW3, and NRRU-TV11) that degrade chlorpyrifos, produce indole-3-acetic acid, and exopolysaccharides under pesticide stress. The results revealed that soil bacteria were identified as Priestia megaterium NRRU-BW3, Bacillus siamensis NRRU-BW9, and Bacillus amyloliquefaciens NRRU-TV11. These strains showed the ability to produce indole-3-acetic acid (IAA) and exopolysaccharides (EPS) in chlorpyrifos. Moreover, these bacteria can degrade chlorpyrifos (CP) in an aqueous medium, and a 33-52% degradation rate was observed after 14 days of incubation. Inoculation with the NRRU-TV11 significantly increased (P < 0.05) plant height, root length, biomass and vigour index of rice seedlings compared to uninoculated controls in chlorpyrifos-contaminated soil. The findings demonstrated the beneficial effects of indigenous NRRU--TV11 on rice seedling development and chlorpyrifos degradation and recommended this strain as a potential replacement for plant growth improvement and environmental bioremediation of pesticide-contaminated agricultural soils.

Sustainable indigenous bio-mixture for restoration the soil point source pollution with special reference to chlorpyrifos

“…Kumari et al ( 2008 ) observed that more than 80% of water samples were contaminated with chlorpyrifos and its residues were above regulatory limits. According to the conclusions of Dar et al ( 2019 ), Chiu et al ( 2021 ), and Saengsanga and Phakratok ( 2023 ), chlorpyrifos had numerous applications worldwide, including as an insecticide, acaricide, and termiticide in homes, public health, and agriculture against a variety of pests, causing chlorpyrifos to contaminate various habitats, including soil, sediments, water, and air. Thus, ongoing studies are being carried out all over the world to create and develop practical and efficient ways to remove chlorpyrifos and related substances from different environmental phases.…”