Biodegradation of Chlorpyrifos (CP) by a Newly Isolated Naxibacter sp. Strain CY6 and Its Ability to Degrade CP in Soil

Abstract

A bacterium, isolated from a vegetable field in a plastic film house and named strain CY6 was capable of biodegrading chlorpyrifos (CP). Based on the phenotypic features and the phylogenetic similarity of 16S rRNA gene sequences, strain CY6 was identified as a Naxibacter sp.. CP was utilized as the sole source of carbon and phosphorus by Naxibacter sp. CY6. We examined the role of this Naxibacter sp. in the degradation of other OP insecticides under liquid cultures. Parathion, methyl parathion, diazinon, cadusafos, and ethoprop could also be degraded by Naxibacter sp. CY6 when they are provided as the sole sources of carbon and phosphorus. Additionally, Naxibacter sp. CY6 (10 8 CFU/g) added to soil with CP (100 mg/kg) resulted in a higher degradation rate of approximately 90% than the rate obtained from uninoculated soils. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide soil.

Chlorpyrifos Removal in an Artificially Contaminated Soil Using Novel Bacterial Strains and Cyclodextrin. Evaluation of Its Effectiveness by Ecotoxicity Studies

“…Naxibacter sp. CY6 [53], Stenotrophomonas sp. YC-1 [54], Pseudomonas putida CBF10-2, Ochrobactrum anthropic FRAF13, and Rhizobium radiobacter GHKF11 were employed to form a bacterial consortium [55], or Achromobacter xylosoxidans JCp4 and Ochrobactrum sp.…”

Remediation of phthalates in river sediment by integrated enhanced bioremediation and electrokinetic process

“…The remaining six strains (i.e., Herminiimonas sp., Zoogloea sp., Naxibacter sp., Massilia sp Actinobacterium, Burkholderiales, and Duganella sp.) have been reported for degradation of other organic contaminants (Li et al, 2005;Kim et al, 2010Kim et al, , 2013Bajaj et al, 2012;Liu et al, 2014;Tong et al, 2015). Although there is no relevant literature showing these strains could degrade PAEs at the present time, however, these strains persistently exist in the environmental media contaminated by PAEs, gradually domesticated and evolved into species that could degrade contaminants in the environment (Wang et al, 2010).…”

Temperature Impacts on Soil Microbial Communities and Potential Implications for the Biodegradation of Turfgrass Pesticides

“…On the other hand, Naxibacter sp. CY6 uses chlorpyrifos as a sole carbon source and produces thiophosphate and 3,5,6‐trichloro‐2‐pyridinol as byproducts (Kim et al, 2013), which are known to retard embryo development in fish (Suvarchala and Philip, 2016). In a similar manner to Naxibacter , Enterobacter species degrade chlorpyrifos to TCP (3,5,6‐trichloropyridinol) in liquid culture conditions, but degradation did not progress beyond this point (Singh et al, 2004).…”