Biodegradation of Cyanide under Alkaline Conditions by a Strain of Pseudomonas Putida Isolated from Gold Mine Soil and Optimization of Process Variables through Response Surface Methodology (RSM)

Moradkhani, Mahboubeh; Yaghmaei, Soheila; Nejad, Zahra Ghobadi

doi:10.3311/ppch.10860

Cited by 18 publications

(8 citation statements)

References 28 publications

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“…Cyanide as the NaCN solution (1 g/L) and glucose (1 g/L) were filter-sterilized before being added into the samples (0.2 mL pore size; Advantec Toyo Kaisha, Ltd., Tokyo, Japan). The NaCN was inserted via a silicone septum into the culture using a syringe [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

Prachumchai

Cherdthong

Wanapat

2021

Veterinary Sciences

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Two experiments were undertaken to screen for ruminal cyanide-utilizing bacteria (Experiment 1), and to evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, gas production parameters, in vitro degradability, and ruminal fermentation (Experiment 2). Experiment 1 was conducted in a completely randomized design (CRD) for the screening of cyanide-utilizing bacteria and the dietary treatments consisted of cyanide at 0, 150, 300, and 450 ppm. In Experiment 2, a 5 × 3 factorial arrangement in a completely randomized design was used for the in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. In Experiment 1, adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p < 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm resulted in increases in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. In Experiment 2, no interaction effects were found between FCR and PELFUR doses on gas production parameters (p > 0.05). Increasing the FCR level to more than 260 g/kg of DM substrate could increase cumulative gas production (p < 0.05). Increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g PELFUR/kg of DM substrate (p < 0.05). The cyanide concentration in rumen fluid decreased with PELFUR (p < 0.05) supplementation. Degradability of in vitro DM and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g PELFUR/kg of DM substrate (p < 0.05) in 440 g FCR/kg of DM substrate. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid among supplementations with FCR (p < 0.05) were significantly different. In conclusion, the present results represent the first finding of bacteria in the rumen that are capable of utilizing cyanide, and suggests that cyanide might function as a nitrogen source for bacterial cell synthesis. The inclusion of FCR of 530 g/kg with 30 g PELFUR/kg of DM substrate could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

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Section: Methodsmentioning

confidence: 99%

Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

Prachumchai

Cherdthong

Wanapat

2021

Veterinary Sciences

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“…Se seleccionaron dos cultivos microbianos denominados JM1 y JM3, con una resistencia hasta 800 ppm NaCN (Figura 1). Este reporte difiere a las diversas cantidades de tolerancia del cianuro investigadas, como Pseudomonas pseudoalcaligenes que mostró crecimiento hasta 780 mg/L a pH de 11,5 y Pseudomonas putida de hasta 500 ppm de cianuro (Luque-almagro et al, 2005;Moradkhani et al, 2018). De los cultivos puros JM1 y JM3 se obtuvieron secuencias de nucleótidos del gen 16S ARNr con 1688 y 1712 pares de bases, respectivamente (Figura 2).…”

Section: Resultados Y Discusiónunclassified

Isolation and molecular characterization of cyanide degrading Halomonas from mining effluents in Peru

Calixto

Rosario

Quinones

et al. 2020

manglar

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“…Cyanide-utilizing bacteria were cultured according to Prachumchai et al 10 . The nutrient medium was prepared according to Kandasamy et al 31 and Moradkhani et al 32 . Briefly, 10 ml of ruminal fluid samples from each animal was diluted with 0.85% sodium chloride to 1:10, 1:100, and 1:1000 and pipetted, spread on agar plates, and incubated at 39 °C for 96 h. Then, cyanide-utilizing bacteria were counted according to the method of Kozaki et al 33 and expressed as cfu/g fresh matter (FM).…”

Section: Ingredientsmentioning

confidence: 99%

Fresh cassava root replacing cassava chip could enhance milk production of lactating dairy cows fed diets based on high sulfur-containing pellet

Prachumchai

Cherdthong

Wanapat

et al. 2022

Sci Rep

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The experiment objective was to assess the shifting effect from cassava chip (CC) to fresh cassava root (FC) affected feed utilization, rumen metabolism, cyanide-using bacteria, and milk quality in lactating Thai Friesian dairy cows fed diets based on high sulfur-containing pellet (PS). Four lactating Thai Friesian dairy cows of 481.5 ± 31.3 kg BW (about 4 years old were allocated with four treatments in a 4 × 4 Latin square design. The four treatments were: replacement FC for CC at 0%, 60%, 80%, and 100% dry matter (DM), respectively. Feed intakes for four diets in terms of total dry matter intake (kg/day and % BW) was linearly correlated with levels of replacement of FC (p < 0.01). Digestibilities of crude protein (CP), DM, organic matter (OM), amylase-treated neutral detergent fiber (aNDF), and acid detergent fiber (ADF) did not change with increasing levels of FC in the diet (p > 0.05). Moreover, the total bacterial counts and cyanide population utilizing bacteria cubically increased with an increase of FC replacement (p < 0.01). The effect of CC substitution with FC in the PS diet was cubically increased on blood thiocyanate concentrations (p < 0.01). In addition, the propionate (C3) concentration at 0 and 4 h post-feeding changed significantly among treatments (p < 0.01), which were linearly improved with an increasing dose of replacement FC and were highest when FC was replaced at 100%. The yield of 3.5% fat-corrected milk was high in the treatment with the replacement of FC as compared to the control (p < 0.01). The yield of fat and milk fat percentages was high (p < 0.01) in the group with the replacement of FC as compared to feed with no diet replaced. The milk thiocyanate concentration was cubically enhanced when levels of FC replacement increased (p < 0.01) and was the highest when FC was replaced at 100%. As the amount of FC replacement was raised, the somatic cell count in the milk decreased linearly (p < 0.01). In conclusion, the replacement of FC at 100% in PS could enhance the feed intake, microbial populations, total volatile fatty acid (VFA), C3 concentration, milk yield, and milk quality.

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Biodegradation of Cyanide under Alkaline Conditions by a Strain of Pseudomonas Putida Isolated from Gold Mine Soil and Optimization of Process Variables through Response Surface Methodology (RSM)

Abstract: (32.23 °C), pH (9.95), and glucose concentration (0.73 g/l).

Cited by 18 publications

References 28 publications

Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

Isolation and molecular characterization of cyanide degrading Halomonas from mining effluents in Peru

Fresh cassava root replacing cassava chip could enhance milk production of lactating dairy cows fed diets based on high sulfur-containing pellet

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