Biodegradation of cypermethrin by immobilized cells of &lt;italic&gt;Micrococcus&lt;/italic&gt; sp. strain CPN 1

Tallur, Preeti N.; Mulla, Sikandar I.; Megadi, Veena B.; Talwar, Manjunatha P.

doi:10.1590/s1517-838246320130557

Cited by 37 publications

(17 citation statements)

References 27 publications

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“…Proteomics study of strain SG4 has confirmed differential protein expressions in the presence of cypermethrin [17]. Other cypermethrin-degrading microbial genera mainly include Pseudomonas, Micrococcus, Acinetobacter, Aspergillus, Trichoderma, and Candida [1,2,[44][45][46]. These microbes use pyrethroids as the sole sources of carbon and nitrogen to fulfill their nutritional requirements [1].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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Cypermethrin is popularly used as an insecticide in households and agricultural fields, resulting in serious environmental contamination. Rapid and effective techniques that minimize or remove insecticidal residues from the environment are urgently required. However, the currently available cypermethrin-degrading bacterial strains are suboptimal. We aimed to characterize the kinetics and metabolic pathway of highly efficient cypermethrin-degrading Bacillus thuringiensis strain SG4. Strain SG4 effectively degraded cypermethrin under different conditions. The maximum degradation was observed at 32 °C, pH 7.0, and a shaking speed of 110 rpm, and about 80% of the initial dose of cypermethrin (50 mg·L−1) was degraded in minimal salt medium within 15 days. SG4 cells immobilized with sodium alginate provided a higher degradation rate (85.0%) and lower half-life (t1/2) of 5.3 days compared to the 52.9 days of the control. Bioaugmentation of cypermethrin-contaminated soil slurry with strain SG4 significantly enhanced its biodegradation (83.3%). Analysis of the degradation products led to identification of nine metabolites of cypermethrin, which revealed that cypermethrin could be degraded first by cleavage of its ester bond, followed by degradation of the benzene ring, and subsequent metabolism. A new degradation pathway for cypermethrin was proposed based on analysis of the metabolites. We investigated the active role of B. thuringiensis strain SG4 in cypermethrin degradation under various conditions that could be applied in large-scale pollutant treatment.

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Section: Discussionmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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“…The increased degradation by immobilized PPO was due to the accelerated activity of PPO by AgNPs. Therefore, the addition of AgNPs to SA–PVA matrix significantly enhanced the degradation of p -cresol and complete degradation was achieved within 18 h. Immobilization provides a kind of membrane protection, which might be responsible for the stabilization of enzyme activity and better degradation rates in the immobilized enzyme (Mulla et al 2012; Hoskeri et al 2014; Tallur et al 2015). The surface morphology of PPO immobilized in SA–PVA–AgNPs bead shows more compact structure when compared with the surface of SA–PVA immobilized beads.…”

Section: Discussionmentioning

confidence: 99%

“…The storage stability and activity of the enzyme in SA–PVA–AgNPs was better than that of encapsulated in other matrices. The SA entrapped PPO showed that with increased number of cycles, the rate of degradation decreased, due to gradual leakage of PPO from these beads (Tallur et al 2015). The thermostability of immobilized PPO enzyme was apparently superior to that of free enzyme.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of p-cresol degradation with polyphenol oxidase (PPO) immobilized in various matrices

et al. 2016

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p-Cresol is an environmental pollutant due to its vast use, toxicity and persistence, nevertheless, its degradation in an enzyme is unclear. In this study, we used Pleurotus sp. isolate VLECK02 polyphenol oxidase (PPO) for the determination of p-cresol degradation. On the basis of UV, FT-IR and chromatographic (HPLC and GC–MS) analysis, 4-methylcatechol was identified as the main metabolite of p-cresol catabolism. In addition, batch and semi-continuous degradation of p-cresol (10 and 20 mM) were studied and compared by free and immobilized PPO in different matrices like sodium alginate (SA), sodium alginate–polyvinyl alcohol (SA–PVA) and sodium alginate–polyvinyl alcohol–silver nanoparticles (SA–PVA–AgNPs). The experimental data showed that an enzyme (PPO) immobilized in SA–PVA–AgNPs was completely degraded p-cresol at initial concentrations of 10 and 20 mM within 30 h. These results suggest that the enzyme immobilized in SA–PVA–AgNPs has achieved higher degradation rates at a given time than free PPO and PPO immobilized in SA–PVA and SA. The SA–PVA–AgNPs and SA–PVA immobilized enzyme could be reused for more than 12 and 8 cycles, respectively, without losing any degradation capacity. Moreover, the immobilized PPO showed higher tolerance to various temperatures and pH than free PPO. Hence, immobilized PPO could be useful for the bioremediation of environment contaminated with phenolic compounds like p-cresol.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0547-y) contains supplementary material, which is available to authorized users.

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“…JQ-41, deltamethrin by Serratia marcescens Del-1, Del-2 and permethrin by Acinetobacter baumannii ZH-14. [11][12][13][14][15][16] Among the commercially available pyrethroids used as insecticides, esfenvalerate is the biologically most active stereoisomer of the pyrethroid fenvalerate. It is very effective against target species (Anticarsia gemmatalis, Scaptocoris castanea and Atarsocoris brachiariae), but it is also toxic to aquatic species, producing ecological imbalances.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of the Pyrethroid Pesticide Esfenvalerate by a Bacterial Consortium Isolated from Brazilian Savannah

Anjos¹,

Birolli²,

Porto³

2020

J. Braz. Chem. Soc.

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New biocatalysts for bioremediation techniques are necessary nowadays. Therefore, a bacterial consortium isolated from Brazilian Savannah was employed for biodegradation of 100 mg L −1 esfenvalerate in liquid culture medium. The bacterial consortium (Lysinibacillus xylanilyticus CBMAI2085, Bacillus cereus CBMAI2067, Lysinibacillus sp. CBMAI2051 and Bacillus sp. CBMAI2052) biodegraded this pyrethroid efficiently. The assays were conducted in triplicate, and after 12 days, 90% of the pesticide was degraded producing 3-phenoxybenzoic acid (35.0 ± 3.1 mg L −1 ) and 2-(4-chlorophenyl)-3-methylbutanoic acid (34.0 ± 2.8 mg L −1 ). The bacterial consortium (52 ± 5% biodegradation) was more efficient in the biodegradation than the average of the same strains solely employed (40 ± 7% biodegradation), showing that the use of consortia is an interesting approach. However, the strain Bacillus cereus CBMAI2067 (67 ± 3% biodegradation) was more efficient than the bacterial consortium, showing its potential as source of carboxylesterases and proving that, in this case, the use of a unique efficient strain is more adequate.

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Biodegradation of cypermethrin by immobilized cells of <italic>Micrococcus</italic> sp. strain CPN 1

Cited by 37 publications

References 27 publications

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Evaluation of p-cresol degradation with polyphenol oxidase (PPO) immobilized in various matrices

Biodegradation of the Pyrethroid Pesticide Esfenvalerate by a Bacterial Consortium Isolated from Brazilian Savannah

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