Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa

Abstract

Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the world, which has a negative effect on human health and the environment. The objective of this research was to investigate the ability of bacterium Pseudomonas aeruginosa in co-culturing with white-rot fungus Pleurotus eryngii to degrade DDT. The various volume of P. aeruginosa (1 ml ≈ 1.5 x 109 CFU) were added into 10 ml of P. eryngii culture for a 7-days of incubation. Approximately 82% of degradation of DDT were obtained from co-cultures with the adjunct of 10 ml of P. aeruginosa during the 7-day incubation period, which had the best ratio of optimization of 0.57. The confrontational assay showed that P. aeruginosa gave no effect on the growth of P. eryngii (0.39 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by co-cultures bacterium P. aeruginosa and fungus P. eryngii. This study indicated that bacterium P. aeruginosa can be used to enhance DDT degradation by whire-rot fungus P. eryngii.

The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum

“…Also, the co-culture of G. lingzhi and P. aeruginosa degraded DDT to 100%, while particular G. lingzhi degraded to 52.52% for 7 days incubation period in the PDB medium [ 39 ]. Other WRFs such as Pleurotus ostreatus and Pleurotus eryngii, which degraded DDT alongside bacterium, were mixed cultures of P. aeruginosa with P. ostreatus , R. pickettii with P. eryngii , and P. aeruginosa with P. eryngii [ [40] , [41] , [42] , [43] , [44] ]. P. ostreatus and P. eryngii with bacterium degraded 86 and 78% of DDT, respectively, while particular P. eryngii and P. ostreatus degraded 43% and 19.35% of DDT, respectively.…”