Biodegradation of di-(2-ethylhexyl) phthalate by novel Rhodococcus sp. PFS1 strain isolated from paddy field soil

Abstract

-phthalate (DEHP) is the phthalate ester frequently utilized as a plasticizer, commonly found in cosmetics, packaging materials; moreover, it has carcinogenic and mutagenic effects on humans. In the current study, we isolated the soil bacterium Rhodococcus sp. PFS1 and to assess its DEHP degradation ability in various environmental conditions. The strain PFS1 was isolated from paddy field soil and identified by the 16S rRNA sequencing analyses. The strain PFS1 was examined for its biodegradation ability of DEHP at various pH, temperature, salt concentration, glucose concentration, and high and low concentrations of DEHP. Moreover, the biodegradation of DEHP at a contaminated soil environment by strain PFS1 was assessed. Further, the metabolic pathway of DEHP degradation by PFS1 was analyzed by HPLC-MS analysis. The results showed that the strain PFS1 effectively degraded the DEHP at neutral pH and temperature 30 °C; moreover, expressed excellent DEHP degradation at the high salt concentration (up to 50 g/L). The strain PFS1 was efficiently degraded the different tested phthalate esters (PAEs) up to 90%, significantly removed the DEHP contamination in soil along with native organisms which are present in soil up to 94.66%; nevertheless, the PFS1 alone degraded the DEHP up to 87.665% in sterilized soil. According to HPLC-MS analysis, DEHP was degraded into phthalate (PA) by PFS1 strain via mono(2ethylehxyl) phthalate (MEHP); then PA was utilized for cell growth. These results suggest that Rhodococcus sp. PFS1 has excellent potential to degrade DEHP at various environmental conditions especially in contaminated paddy field soil.

Remediation of Leachate-Metal-Contaminated Soil Using Selected Bacterial Consortia

“…The aerobic, irregularly rod-shaped and Gram-positive bacteria Rhodococcus wratislaviensis was also isolated from the leachate-contaminated site. It is predominant in soils [55,56]. Ref.…”

Exploring Potent Fungal Isolates from Sanitary Landfill Soil for In Vitro Degradation of Dibutyl Phthalate

“…Microorganisms employ a diverse range of enzyme compositions consisting of esterases, dioxygenases, cutinase, etc., and they play a crucial role in degradation [ 20 ]. In previous studies, various bacterial genera of Delftia, Enterobacter , Agrobacterium, Variovorax, Micrococcus, Gordonia, Methylobacillus, Rhizobium, Bacillus and Rhodococcus have been reported to degrade phthalates [ 21 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Among fungi, Polyporus, Fusarium and Pleurotus have been studied previously [ 38 , 39 , 40 ].…”

Rhodococcus strains as a good biotool for neutralizing pharmaceutical pollutants and obtaining therapeutically valuable products: Through the past into the future

“…are capable of oxidation and complete degradation of a wide range of organic compounds, recalcitrant pollutants and toxic compounds, pesticides, emerging pollutants, endocrine disruptors, explosives, flame retardants, plasticizers, defoliants, and plastics, including microplastics. Additionally, they have potential for fuel biodesulfurization, lipid synthesis, and biofuel production ( Coleman et al, 2002 ; Gilan and Sivan, 2013 ; Auta et al, 2018 ; Presentato et al, 2018b ; Pang et al, 2020 ; Alvarez et al, 2021 ; Hirschler et al, 2021 ; Keasling et al, 2021 ; Yu et al, 2021 ; Baek et al, 2022 ; Kamaraj et al, 2022 ). The biochemical potential of rhodococci is intensively studied due to their catabolic flexibility, unique enzymatic capabilities, broad substrate specificity and an effective set of survival strategies ( Larkin et al, 2010 ; Cappelletti et al, 2019 ; de Carvalho, 2019 ; Kuyukina and Ivshina, 2019a ).…”