Biodegradation of Di-n-Butyl Phthalate by a Newly Isolated Halotolerant Sphingobium sp.

Abstract

A Gram-negative strain (TJ) capable of growing aerobically on mixed phthalate esters (PAEs) as the sole carbon and energy source was isolated from the Haihe estuary, Tianjin, China. It was identified as belonging to the Sphingobium genus on the basis of morphological and physiological characteristics and 16S rRNA and gyrb gene sequencing. The batch tests for biodegradation of di-n-butyl phthalate (DBP) by the Sphingobium sp. TJ showed that the optimum conditions were 30 °C, pH 7.0, and the absence of NaCl. Stain TJ could tolerate up to 4% NaCl in minimal salt medium supplemented with DBP, although the DBP degradation rates slowed as NaCl concentration increased. In addition, substrate tests showed that strain TJ could utilize shorter side-chained PAEs, such as dimethyl phthalate and diethyl phthalate, but could not metabolize long-chained PAEs, such as di-n-octyl phthalate, diisooctyl phthalate, and di-(2-ethyl-hexyl) phthalate. To our knowledge, this is the first report on the biodegradation characteristics of DBP by a member of the Sphingobium genus.

Impacts of dimethyl phthalate on the bacterial community and functions in black soils

“…Commonly, DMP can migrate into soils via irrigation and the application of pesticides and plastic films (Cartwright et al, 2000 ; Erythropel et al, 2012 ; Wang et al, 2014 ). Considering its mutagenicity, teratogenicity and carcinogenicity, both the United States Environmental Protection Agency and the China State Environmental Protection Administration have listed DMP as an environmental priority pollutant (Jin et al, 2013 ).…”

Draft Genome Sequence of Sphingobium yanoikuyae TJ, a Halotolerant Di- n -Butyl-Phthalate-Degrading Bacterium

“…S. yanoikuyae TJ could degrade di- n -butyl phthalate (DBP), one of the most used phthalate esters as a sole carbon and energy source and a pollutant of concern in various environments. The characteristics of S. yanoikuyae TJ for biodegradation of DBP were reported in our previous study ( 2 ). So far, the genome sequences of several Sphingobium spp., such as Sphingobium sp.…”

Biodegradation of di-n-Butyl Phthalate by Achromobacter sp. Isolated from Rural Domestic Wastewater

“…Moreover, W-1 also used phenol, which is a widespread pollutant worldwide. Based on these results, DBP, which is one of most popular plasticizers, was used in further degradation tests [ 28 ].…”