Biodegradation of dibutyl phthalate and diethyl phthalate by indigenous isolate Bacillus sp. MY156: Characterization, stoichiometry, enzyme activity, and physiological response to cell surface

Aeration forms a critical part of the biodrying of refuse-derived fuel-3 (RDF-3) and significantly affects the fuel’s energy potential. Understanding the organic content (OC) of RDF-3 is crucial for determining the optimal aeration strategy. In this study, we conducted a bio-methane potential (BMP) test to estimate the OC by observing the conversion of organic matter into methane (CH₄) and carbon dioxide (CO₂). The observation of BMP was conducted using anaerobic digestion approach where substrate and inoculum are important parameters considered for the success of this test. Various ratios substrate-to-inoculum (S/I) were explored to assess their impact on biogas production, our research involved testing four S/I ratios (0.25, 0.5, 1.0, and 1.5) focusing on identifying the optimal aeration strategy. Based on stoichiometric calculations, the sample’s biogas yield per gram volatile solid indicates RDF-3’s OC is 1.5%. This OC value played a role in establishing the appropriate aeration rate (AR) for the biodrying process, which was determined to be 0.6 m³/kg.day, indicating the action of effective microbial degradation processes. Ensuring the correct AR is vital for maximizing the energy potential of RDF-3. Implementing optimized aeration rates based on the BMP test in waste management practices can significantly improve RDF-3 biodrying efficiency. This approach enhances RDF quality, reduces moisture, increases calorific value, and minimizes greenhouse gas emissions, leading to more sustainable and efficient waste-to-energy conversion.

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Biodegradation of dibutyl phthalate and diethyl phthalate by indigenous isolate Bacillus sp. MY156: Characterization, stoichiometry, enzyme activity, and physiological response to cell surface

Cited by 3 publications

References 62 publications

Harnessing landfill-derived Bacillus subtilis (LLS-04) for bio-electrodegradation of di-butyl phthalate: Comprehensive toxicity assessment across multiple biological models

Harnessing landfill-derived Bacillus subtilis (LLS-04) for bio-electrodegradation of di-butyl phthalate: Comprehensive toxicity assessment across multiple biological models

Application of cold-adapted microbial agents in soil contaminate remediation: biodegradation mechanisms, case studies, and safety assessments

Optimizing aeration rates via bio-methane potential test for enhanced biodrying efficiency of refuse-derived fuel-3

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