Biodegradation of diphenyl ether herbicide lactofen by Bacillus sp. YS-1 and characterization of two initial degrading esterases

The long-term and widespread use of diphenyl ether herbicides has caused serious soil residue problems and threatens the agricultural ecological environment. The development of biodegrading agents using high-efficiency degrading strains as pesticide residue remediation materials has been widely recognized. In this study, the strain Bacillus sp. Za was used to prepare solid agents for the remediation of diphenyl ether herbicides-contaminated soil. The ratio of organic fertilizer was 1:3 (pig manure: cow dung), the inoculum amount of Za was 10%, the application amount of solid agents was 7%, and the application mode was mixed application, all of which were the most suitable conditions for solid agents. After the solid agents were stored for 120 days, the amount of Za remained above 108 CFU/g. The degradation rates of the solid agents for lactofen, bifenox, fluoroglycofen, and fomesafen in soil reached 87.40, 82.40, 78.20, and 65.20%, respectively, on the 7th day. The application of solid agents alleviated the toxic effect of lactofen residues on maize seedlings. A confocal laser scanning microscope (CLSM) was used to observe the colonization of Za-gfp on the surface of maize roots treated in the solid agents, and Za-gfp mainly colonized the elongation zone and the mature area of maize root tips, and the colonization time exceeded 21 days. High-throughput sequencing analysis of soil community structural changes in CK, J (solid agents), Y (lactofen), and JY (solid agents + lactofen) groups showed that the addition of solid agents could restore the bacterial community structure in the rhizosphere soil of maize seedlings. The development of solid agents can facilitate the remediation of soil contaminated with diphenyl ether herbicide residues and improve the technical level of the microbial degradation of pesticide residues.

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“…( Hu et al, 2020 ) and Bacillus sp. YS-1 ( Shang et al, 2022 ). The research object of this study, Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus sp. YS-1 was shown to degrade 50 mg/l lactofen at an efficiency rate that reached 97.6% within 15 h ( Shang et al, 2022 ). Additionally, Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

Zhao

Tian

et al. 2022

Front. Microbiol.

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“…The reported microorganisms that have degradation ability mainly include Aspergillus niger, Lysinibacillus, Shigella flexneri, Stenotrophomonas acidaminiphila, Pseudomonas zeshuii, etc. [2,[22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39]. In addition to the degradation of major diphenyl ether herbicides, some diphenyl ether derivatives have also been reported.…”

Section: Progress In Microbial Degradation Of Diphenyl Ether Herbicid...mentioning

confidence: 99%

“…Hydrolases, esterase, and oxidoreductases are the typical enzymes involved in the degradation of chemical pesticides such as diphenyl ether herbicides [ 44 ]. For instance, Shang et al [ 34 ] discovered that Bacillus sp. YS-1 degraded lactofen to three degradation products under the action of esterase RapE and RhoE .…”

Section: Bioremediation Of Residual Contamination Of Diphenyl Ether H...mentioning

confidence: 99%

A Review of Remediation Strategies for Diphenyl Ether Herbicide Contamination

Fan,

Shen,

Yang

et al. 2024

Toxics

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In agriculture, diphenyl ether herbicides are a broad-spectrum family of pesticides mainly used to control annual weeds in agriculture. Although diphenyl ether herbicides have a long-lasting effect in weed control, they can also be harmful to succeeding crops, as well as to the water and soil environment. Residual herbicides can also harm a large number of non-target organisms, leading to the death of pest predators and other beneficial organisms. Therefore, it is of great significance to control and remediate the contamination caused by diphenyl ether herbicide residues for the sake of environmental, nutritional, and biological safety. This review provides an overview of the techniques used for remediating diphenyl ether herbicide contamination, including biological, physical, and chemical remediation. Among these techniques, bioremediation, particularly microbial biodegradation technology, is extensively employed. The mechanisms and influencing factors of different remediation techniques in eliminating diphenyl ether herbicide contamination are discussed, together with a prospect for future development directions. This review serves as a scientific reference for the efficient remediation of residual contamination from diphenyl ether herbicides.

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“…The degradation process of diphenyl ether herbicides primarily involves the cleavage of dichlorination, 7 the acetylation of amino derivatives, 8 ether bond cleavage, 9 nitroreduction, 10 and ester bonds. 11 Numerous degrading genes and enzymes have been successfully cloned and expressed. The potential environmental impact of acifluorfen poses a significant concern, and the reduction of nitro is a crucial step in mitigating the harm caused by acifluorfen.…”

Section: Introductionmentioning

confidence: 99%

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

Zhao,

Wang,

Tian

et al. 2024

J. Agric. Food Chem.

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The residues of acifluorfen present a serious threat to the agricultural environment and sensitive crops. DnrA, a nitroreductase, is an intracellular enzyme that restricts the application of wild-type Bacillus sp. Za in environmental remediation. In this study, two strategies were employed to successfully secrete DnrA in strains SCK6 and Za, and the secretion expression conditions were optimized to achieve rapid degradation of acifluorfen. Under the optimal conditions, the relative activities of the DnrA supernatant from strains SCK6-D and Za-W were 3.06-fold and 3.53-fold higher than that of strain Za, respectively. While all three strains exhibited similar tolerance to different concentrations of acifluorfen, strains SCK6-D and Za-W demonstrated significantly faster degradation efficiency compared to strain Za. Furthermore, the DnrA supernatant from strains SCK6-D and Za-W could effectively reduce the toxicity of acifluorfen on maize and cucumber seedlings. This study provides an effective technical approach for the rapid degradation of acifluorfen.

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Biodegradation of diphenyl ether herbicide lactofen by Bacillus sp. YS-1 and characterization of two initial degrading esterases

Cited by 11 publications

References 30 publications

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

A Review of Remediation Strategies for Diphenyl Ether Herbicide Contamination

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

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