Biodegradation of dispersed Macondo crude oil by indigenous Gulf of Mexico microbial communities

Wang, Jian; Sandoval, Kathia; Ding, Yan; Stoeckel, Donald M.; Minard-Smith, Angela; Andersen, Gary L.; Dubinsky, Eric A.; Atlas, Ronald M.; Gardinali, Piero R.

doi:10.1016/j.scitotenv.2016.03.015

Cited by 79 publications

(64 citation statements)

References 24 publications

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“…Oceanospirillaceae is associated with alkane metabolism (Hazen et al 2010;Redmond and Valentine 2012;Dubinsky et al 2013;Brakstad et al 2015b;Wang et al 2016), in agreement with the abundances early during the oil biodegradation period. This family was represented by the genus Oleispira, known to biodegrade alkanes at low seawater temperatures (Yakimov et al 2003;Coulon et al 2007;Golyshin et al 2010;Lofthus et al 2018).…”

Section: Discussionsupporting

confidence: 76%

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

et al. 2018

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With increasing oil exploration, production and transportation in the Arctic, predictions of the fate of spilled oil become important, including oil compound biodegradation. The use of chemical dispersants may result in increased biodegradation due to the generation of dispersions consisting of small oil droplets, but only few studies have focused on biodegradation of chemically dispersed oil in Arctic seawater. In this study we compared oil biotransformation in Arctic and temperate seawaters collected from Western Greenland (Disko Bay, surface and from 80 m depth) and a Norwegian fjord (Trondheimsfjord, surface). A naphthenic oil, premixed with a dispersant, was dispersed in the seawaters from the different sources, and the dispersions incubated in low concentrations in a carousel system at 4-5 °C for up to 64 days. Targeted oil compounds (n-alkanes, BTEX, naphthalenes and PAHs) were biotransformed in both Arctic and temperate seawaters, although the degradation was faster in the temperate seawater. In the Arctic seawater, transformation was faster in the surface than in the subsurface seawater. Calculations of biotransformation rates and half-lives of oil compound groups representing 70-80% of fresh oils also showed significantly faster depletion in the temperate than the Arctic seawater. Microbial analyses revealed differences between the bacterial communities in the seawater sources during oil biodegradation. The results emphasized, that oil compounds are biodegraded in Arctic seawater, but degradation potential and rates may vary between seawaters from different sources.

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Section: Discussionsupporting

confidence: 76%

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

et al. 2018

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“…Biodegradation of aromatic compounds obtained in this work was related to the degree of alkylation, with the non‐alkylated compound being degraded first (naphthalene), followed by the least alkylated compounds. These results similar to those obtained by other authors, confirm that increases in the number of methyl substituents reduces degradation rates (Leblond et al ; Wang et al ).…”

Section: Discussionsupporting

confidence: 92%

Bacterial succession and co‐occurrence patterns of an enriched marine microbial community during light crude oil degradation in a batch reactor

Uribe-Flores

Cerqueda-García

Hernández-Nuñéz

et al. 2019

J of Applied Microbiology

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Aims The aim of this study was to investigate the dynamic changes in the bacterial structure and potential interactions of an acclimatized marine microbial community during a light crude oil degradation experiment. Methods and Results The bacterial community effectively removed 76·49% of total petroleum hydrocarbons after 30 days, as evidenced by GC‐FID and GC‐MS analyses. Short‐chain alkanes and specific aromatic compounds were completely degraded within the first 6 days. High‐throughput sequencing of 16S rRNA gene indicated that the starting bacterial community was mainly composed by Marinobacter and more than 30 non‐dominant genera. Bacterial succession was dependent on the hydrocarbon uptake with Alcanivorax becoming dominant during the highest degradation period. Sparse correlations for compositional data algorithm revealed one operational taxonomic unit (OTU) of Muricauda and an assembly of six OTUs of Alcanivorax dieselolei and Alcanivorax hongdengensis as critical keystone components for the consortium network maintenance and stability. Conclusions This work exhibits a stabilized marine bacterial consortium with the capability to efficiently degrade light crude oil in 6 days, under laboratory conditions. Successional and interaction patterns were observed in response to hydrocarbon consumption, highlighting potential interactions between Alcanivorax and keystone non‐dominant OTUs over time. Significance and Impact of the Study Our results contribute to the understanding of interactions and potential roles of specific members of hydrocarbonoclastic marine bacterial communities, which will be useful for further bioaugmentation studies concerning the associations between indigenous and introduced micro‐organisms.

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“…This study used a laboratory-based method to recreate the conditions that were present in the deep ocean in the Gulf of Mexico and recapitulate the hydrocarbon degradation pattern and microbial community succession observed in the 2010 DWH event and partially observed during an incomplete time course in an earlier laboratory simulation (15). The combination of cold temperature, lack of light, bioavailable oil in the form of 10-μm droplets, dispersant, and the indigenous microbial community allowed us to successfully resolve high-quality genomes and their functional capacities for all key microbes implicated throughout the entire DWH plume oil degradation time course.…”

Section: Discussionmentioning

confidence: 99%

“…Natural seawater collected from Mississippi Canyon block MC-294 at 1,100-to 1,200-m depth was mixed to a final concentration of 2 ppm oil and 0.02 ppm Corexit EC9500A dispersant. Microdroplets were produced using a pressurized flow injection (PFI) droplet generator (15) to produce median-size oil droplets of 10-μm diameter using Macondo (MC252) oil (SI Methods). Oil droplets remained in suspension in replicate 2-L bottles for the 64 d of the experiment.…”

Section: Microbial Community Structural Changes Correlated With Hydromentioning

confidence: 99%

“…Many studies of the plume samples reported that the structure of the microbial communities shifted as time progressed (3)(4)(5)(6)(11)(12)(13)(14)(15)(16)). Member(s) of the order Oceanospirillales dominated from May to mid-June, after which their numbers rapidly declined and species of Cycloclasticus and Colwellia dominated for the next several weeks (4,5,14).…”

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confidence: 99%

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Simulation of Deepwater Horizon oil plume reveals substrate specialization within a complex community of hydrocarbon degraders

Dubinsky

Probst

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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115

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The Deepwater Horizon (DWH) accident released an estimated 4.1 million barrels of oil and 10 10 mol of natural gas into the Gulf of Mexico, forming deep-sea plumes of dispersed oil droplets and dissolved gases that were largely degraded by bacteria. During the course of this 3-mo disaster a series of different bacterial taxa were enriched in succession within deep plumes, but the metabolic capabilities of the different populations that controlled degradation rates of crude oil components are poorly understood. We experimentally reproduced dispersed plumes of fine oil droplets in Gulf of Mexico seawater and successfully replicated the enrichment and succession of the principal oil-degrading bacteria observed during the DWH event. We recovered near-complete genomes, whose phylogeny matched those of the principal biodegrading taxa observed in the field, including the DWH Oceanospirillales (now identified as a Bermanella species), multiple species of Colwellia, Cycloclasticus, and other members of Gammaproteobacteria, Flavobacteria, and Rhodobacteria. Metabolic pathway analysis, combined with hydrocarbon compositional analysis and species abundance data, revealed substrate specialization that explained the successional pattern of oildegrading bacteria. The fastest-growing bacteria used short-chain alkanes. The analyses also uncovered potential cooperative and competitive relationships, even among close relatives. We conclude that patterns of microbial succession following deep ocean hydrocarbon blowouts are predictable and primarily driven by the availability of liquid petroleum hydrocarbons rather than natural gases.hydrocarbon biodegradation | Gulf of Mexico | microbial communities | Macondo oil | genome succession

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Biodegradation of dispersed Macondo crude oil by indigenous Gulf of Mexico microbial communities

Cited by 79 publications

References 24 publications

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

Bacterial succession and co‐occurrence patterns of an enriched marine microbial community during light crude oil degradation in a batch reactor

Simulation of Deepwater Horizon oil plume reveals substrate specialization within a complex community of hydrocarbon degraders

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