Biodegradation of Endosulfan by Klebsiella oxytoca KE-8 Immobilized on Activated Carbon

Abstract

Endosulfan degrading ability of(dry weight) degraded 22.18 ug ml -1 endosulfan within 5 days at pH 7.0, 30℃ in batch shake flask cultures. Also, we an experimented recycle packed bed column mode and continuous packed bed column mode for endosulfan degradation. Under optimum operation condition, the immobilized cells in a laboratory scale pack bed column with support beads were able to degrade endosulfan completely in defined minimal salt medium at a maximum rate of 129.6 ug ml -1 per day. Moreover, the endosulfan degradation activity could be demonstrated at 4℃ for one month without significant decrease in activity. Results of this study suggest that immobilized cells of Klebsiella oxytoca KE-8 might be applicable to endosulfan contaminated site.

Use of Ca-alginate immobilized Pseudomonas aeruginosa for repeated batch and continuous degradation of Endosulfan

“…aeruginosa were able to show 60 % degradation of Endosulfan at the end of the 35th cycle with a cell leakage of 642 × 10 4 Cfu/mL. In similar studies, Endosulfan degrading ability of Klebsiella oxytoca KE-8 immobilized by entrapment with activated carbon was investigated by Jo et al ( 2010 ). Activated carbon with immobilized cells K. Oxytoca KE-8 that had been used for one degradation activity was stored for 1, 15, and 30 days at 4 °C.…”

“…With 10 % concentration of pesticide at 100 mL/h flow rate, 85 % degradation of Endosulfan was recorded. Recycle packed bed column mode and continuous packed bed column mode for Endosulfan degradation were also studied by Jo et al ( 2010 ). The effect of the hydraulic retention time (from 25 to 300 min) on the performance of bioreactor was studied by them with variation in the influent flow rate.…”

“…There are reports on the biodegradation of pesticides using immobilized cells (Ha et al 2009 ; Yáñez-Ocampo et al 2011 ; Abdel-Razek et al 2013 ) and immobilized enzymes (Richins et al 2000 ; Mansee et al 2005 ). However, studies on the degradation of Endosulfan using immobilized cells are limited (Jo et al 2010 ).…”