Biodegradation of Extractable Petroleum Hydrocarbons by Consortia &lt;i&gt;Bacillus cereus&lt;/i&gt; and &lt;i&gt;Pseudomonas putida&lt;/i&gt; in Petroleum Contaminated-Soil

Tuhuloula, Abubakar; Suprapto, Suprapto; Altway, Ali; Juliastuti, Sri Rachmania

doi:10.22146/ijc.33765

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…has resistant endospores to be more tolerant to high levels of hydrocarbons in soil that has been proved according to the research [34]. High biodegradation capacity at the level above 93 % of crude oil was recorded for the bacterial consortium of Bacillus cereus and Pseudomonas putida bacteria [35].…”

Section: Modeling Of Oil-degradable Bacteria Consortium Capable For B...mentioning

confidence: 85%

Decontamination of Oil-Polluted Soils: Power of Electronic Bioinformatic Databases

Ablіeіeva

Plyatsuk

Liu

et al. 2022

JES

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The main idea was to solve the problem related to oil contamination of soil using bioremediation with bioaugmentation with modeled microorganism strains. The paper aimed to develop a bacterial consortium for petroleum hydrocarbon degradation during the biological treatment of oil-contaminated soils using electronic databases. The research methodology included an analysis of the mechanisms and metabolic pathways of petroleum hydrocarbon degradation and an assessment of the possible reaction modulus and enzymatic systems for the degradation of aromatic compounds. The taxonomic classification and review of oil compound transformation metabolic pathways were carried out using electronic KEGG, MetaCyc, and EzTaxon databases. The KEGG database was used to create a microbiological consortium of certain strains of bacteria that improved hydrocarbon degradation process performance. Identification of bacteria’s complete genome using Island Viewer 4 allowed to create of a consortium of oil-destructive bacteria consisting of such strains: Pseudoxanthomonas spadix BD-a59, Rhodococcus jostii RHA1, Rhodococcus aetherivorans IcdP1, Pseudomonas putida ND6, Pseudomonas stutzeri 19SMN4, Pseudomonas fluorescens UK4, Acinetobacter lactucae OTEC-02, Bacillus cereus F837/76.7.9. The ratio between the mentioned strains of microorganisms in the consortium was set at 20 % : 20 % : 15 % : 10 % : 10 % : 5 % : 5 % : 15 %. This bacterial consortium for aromatic hydrocarbons was created according to the metabolic information of basic enzymatic systems and the predominant transformation of particular oil compounds using the BacDive database.

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Section: Modeling Of Oil-degradable Bacteria Consortium Capable For B...mentioning

confidence: 85%

Decontamination of Oil-Polluted Soils: Power of Electronic Bioinformatic Databases

Ablіeіeva

Plyatsuk

Liu

et al. 2022

JES

View full text Add to dashboard Cite

show abstract

“…has resistant endospores to be more tolerant to high levels of hydrocarbons in soil that has been proved according to the research (Kidibule et al, 2014). High biodegradation capacity at the level above 93% of crude oil was recorded for the bacterial consortium of Bacillus cereus and Pseudomonas putida bacteria (Tuhuloula et al, 2019). Jussila et al (2007) reported Pseudomonas aeruginosa JI104 must be an effective utilizing and catechol-positive bacteria having the xylEgene participated in the destruction of aromatic hydrocarbons (xylE, BTEX destruction).…”

Section: Modeling Of Oil-degradable Bacteria Consortium Capable For B...mentioning

confidence: 88%

Degradation of Polycyclic Aromatic Hydrocarbons During Decontamination of Oil-Polluted Soils: In Silico Approach

Ablіeіeva

Plyatsuk

Liu

et al. 2022

Preprint

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The main idea was to solve the problem related to oil contamination of soil using bioremediation with bioaugmentation with modelled microorganism strains. The paper aimed to develop a bacterial consortium for petroleum hydrocarbons degradation during biological treatment of oil-contaminated soils using electronic databases. The methodology of the research included analysis of the mechanisms and metabolic pathways of petroleum hydrocarbons degradation, and assessment of the possible reaction modulus and enzymatic systems for degradation of aromatic compounds. The taxonomic classification and review of metabolic pathways of oil compound transformation were carried out using electronic KEGG, MetaCyc and EzTaxon databases. The KEGG database was used to create a microbiological consortium of certain strains of bacteria that improved hydrocarbon degradation process performance. Identification of bacteria complete genome using Island Viewer 4 allowed to create of a consortium of oil-destructive bacteria consisting of such strains: Pseudoxanthomonas spadix BD-a59, Rhodococcus jostii RHA1, Rhodococcus aetherivorans IcdP1, Pseudomonas putida ND6, Pseudomonas stutzeri 19SMN4, Pseudomonas fluorescens UK4, Acinetobacter lactucae OTEC-02, Bacillus cereus F837/76.7.9. The ratio between the mentioned strains of microorganisms in the consortium was set at 20% : 20% : 15% : 10% : 10% : 5% : 5% : 15%. This bacterial consortium for aromatic hydrocarbons was created according to the metabolic information of basic enzymatic systems and predominant transformation of particular oil compounds, using BacDive database.

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“…Penurunan konsentrasi xylene sejalan dengan kenaikan populasi bakteri di dalam bioreaktor. Hasil yang sama juga didapatkan pada penelitian mengenai biodegradasi EPH dengan menggunakan konsorsium Bakteri, dimana bakteri memanfaatkan hidrokarbon sebagai support growth [10]. Semakin meningkat populasi bakteri maka akan menurunkan jumlah substrat di dalam bioreaktor.…”

Section: Kondisi Awal Tanah Tercemar Minyak Bumiunclassified

Biodegradasi Xylene Dari Lahan Tercemar Minyak Bumi Oleh Bakteri Rhodococcus Erythropolis

Ole,

Juliastuti

2023

JTKM

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Peningkatan eksploitasi minyak bumi merupakan sumber utama pencemaran tanah dan air. Tanah yang tercemar hidrokarbon pada dasarnya telah mengandung mikroorganisme yang dapat memecah hidrokarbon. Akan tetapi proses biodegradasi hidrokarbon dengan metode in situ mengalami hambatan karena lahan tercemar yang luas dan kebutuhan nutrien yang tidak terpenuhi. Oleh sebab itu maka dikembangkan metode ex situ dengan memindahkan tanah tercemar ke laboratorium untuk diolah dengan metode slurry bioreaktor. Mikroorganisme yang digunakan dalam proses ini adalah bakteri aerob Rhodococcus erythropolis yang mampu menguraikan alkilbenzena, seperti o-xylena, toluene dan etilbenzena pada temperatur 25oC – 37oC. Proses degradasi dilakukan pada 3 bioreaktor berpengaduk untuk konsentrasi baktri 12,5%, 15,0% dan 17,5%. Monitoring populasi bakteri dilakukan setiap 2 hari untuk mengetahui fase hidup mikroorganisme. Analisis hidrokarbon dilakukan setiap 14 hari selama 8 minggu dengan metode gas chromatography (GC). Bioreaktor 17,5% Rhodococcus erythropolis memberikan persen degradasi paling baik yaitu 96,83%.

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Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil

Cited by 11 publications

References 18 publications

Decontamination of Oil-Polluted Soils: Power of Electronic Bioinformatic Databases

Decontamination of Oil-Polluted Soils: Power of Electronic Bioinformatic Databases

Degradation of Polycyclic Aromatic Hydrocarbons During Decontamination of Oil-Polluted Soils: In Silico Approach

Biodegradasi Xylene Dari Lahan Tercemar Minyak Bumi Oleh Bakteri Rhodococcus Erythropolis

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