Biodegradation of fibrillated oil palm trunk fiber by a novel thermophilic, anaerobic, xylanolytic bacterium Caldicoprobacter sp. CL-2 isolated from compost

Widyasti, Erma; Shikata, Ayumi; Hashim, Rokiah; Sulaiman, Othman; Sudesh, Kumar; Wahjono, Edi; Kosugi, Akihiko

doi:10.1016/j.enzmictec.2017.12.009

Cited by 35 publications

(11 citation statements)

References 31 publications

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“…Instead, the anaerobic Caldicoprobacter sp. CL-2, isolated from bovine manure compost, has a xylanase activity showing a modular structure with a glycoside hydrolase domain coupled with a carbohydrate binding module [ 149 ]. Another hyperthermophilic microorganism from geothermal springs that produce miscellaneous glycoside hydrolases is T. maritima ; it has an endoglucanase enzyme (Tm_Cel5A) with an optimum T of 80 °C and pH of 4.8.…”

Section: Lignocellulosic Biomassesmentioning

confidence: 99%

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Gallo

Puopolo

Carbonaro

et al. 2021

IJERPH

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Extremophiles are microorganisms that populate habitats considered inhospitable from an anthropocentric point of view and are able to tolerate harsh conditions such as high temperatures, extreme pHs, high concentrations of salts, toxic organic substances, and/or heavy metals. These microorganisms have been broadly studied in the last 30 years and represent precious sources of biomolecules and bioprocesses for many biotechnological applications; in this context, scientific efforts have been focused on the employment of extremophilic microbes and their metabolic pathways to develop biomonitoring and bioremediation strategies to face environmental pollution, as well as to improve biorefineries for the conversion of biomasses into various chemical compounds. This review gives an overview on the peculiar metabolic features of certain extremophilic microorganisms, with a main focus on thermophiles, which make them attractive for biotechnological applications in the field of environmental remediation; moreover, it sheds light on updated genetic systems (also those based on the CRISPR-Cas tool), which expand the potentialities of these microorganisms to be genetically manipulated for various biotechnological purposes.

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Section: Lignocellulosic Biomassesmentioning

confidence: 99%

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Gallo

Puopolo

Carbonaro

et al. 2021

IJERPH

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“…The compost included sugarcane bagasse, rice husk and OPEN ACCESS bovine faeces. The samples were collected 10 to 20 cm below the surface at temperatures between 60 and 75 °C and placed in oxygen-starved Ziploc bags (Johnson) [4,5]. The samples were stored at -80 °C until initiating the enrichment culture required for species isolation.…”

Section: Isolation and Ecologymentioning

confidence: 99%

Capillibacterium thermochitinicola gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost

Ungkulpasvich

Baramee

Uke

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-negative, spore forming, obligately anaerobic, thermophilic, chitin-degrading bacterium, designated UUS1-1T, was isolated from compost on Ishigaki Island, Japan by enrichment culturing using chitin powder as the carbon source. The strain has unique, long, hair-like rod morphological features and exhibits strong degradation activity toward crystalline chitin under thermophilic conditions. Growth of the novel strain was observed at 45–65 °C (optimum, 55 °C) and pH 6.5–7.5 (optimum, pH 7.0). In addition to chitin, the strain utilized several other carbon sources, including N-acetylglucosamine, glucose, galactose, mannose, maltose, cellobiose, fructose and sucrose. The end products of chitin degradation were acetate, lactate, H2 and CO2. Phylogenetic tree analysis based on 16S rRNA gene sequences revealed a clear affiliation of the proposed bacterium to the phylum Firmicutes ; the most closely related species were Hydrogenispora ethanolica LX-BT and Desulfotomaculum thermobenzoicum DSM6193T with similarities of 90.4 and 87.8 %, respectively. The G+C content of the genomic DNA was 52.1 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of UUS1-1T and H. ethanolica LX-BT were 65.5 and 21.0 %, respectively. The cellular fatty acid composition of the strain was C16 : 0, anteiso-C15 : 0, C14 : 0, C12 : 0 3-OH and dimethyl acetal-C13 : 0. Based on phenotypic, chemotaxonomic and genotypic analysis, strain UUS1-1T represents a novel genus and species, for which the name Capillibacterium thermochitinicola gen. nov., sp. nov. is proposed. The type strain is UUS1-1T (=JCM 33882T=DSM 111537T).

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“…The thermophilic anaerobic bacterium Clostridium thermocellum (recently called Hungateiclostridium thermocellum ) is a multifunctional ethanol producer, capable of both saccharification and fermentation [1] . C. thermocellum PAL5 was derived from strain S14 [2], [3], [4] that was isolated from bagasse paper sludge. The cellulolytic activity of strain PAL5 was compared with those of C. thermocellum ATCC27405 T , a type strain of this species [5], and C. thermocellum DSM1313 [6] by incubation for 3 days at 60 °C in CTFUD medium [7] containing 1.0% microcrystalline cellulose powder instead of cellobiose.…”

Section: Datamentioning

confidence: 99%

Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability

et al. 2019

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Clostridium thermocellum is a potent cellulolytic bacterium. C. thermocellum strain PAL5, was derived from strain S14 that was isolated from bagasse paper sludge, possesses higher cellulose-degradation ability than representative strains ATCC27405 and DSM1313. In this work, we determined the draft genome sequence of C. thermocellum PAL5. Genomic DNA was used for whole-genome sequencing using the Illumina HiSeq 2500. We obtained 215 contigs of >200 bp (N50, 78,366 bp; mean length, 17,378 bp). The assembled data were subjected to the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline, and 3198 protein-coding sequences, 53 tRNA genes, and 4 rRNA genes were identified. The data are accessible at NCBI (the accession number SBHL00000000). Our data resource will facilitate further studies of efficient cellulose-degradation using C. thermocellum .

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Biodegradation of fibrillated oil palm trunk fiber by a novel thermophilic, anaerobic, xylanolytic bacterium Caldicoprobacter sp. CL-2 isolated from compost

Cited by 35 publications

References 31 publications

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering

Capillibacterium thermochitinicola gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost

Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability

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