Biodegradation of Halogenated Hydrocarbon Fumigants by Nitrifying Bacteria

Rasche, Madeline E.; Hyman, Michael R.; Arp, Daniel J.

doi:10.1128/aem.56.8.2568-2571.1990

Cited by 87 publications

(51 citation statements)

References 15 publications

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“…Although it is known that AMO of N. europaea can oxidize aromatic compounds, we found that AMO of N. oceanus is unable to oxidize styrene or ethylbenzene (data not shown). This is consistent with the ability of AMO in N. oceanus to oxidize the smaller substrates ethylene and methylbromide, but not 1,2 dibromochloropropane or 1,2 dichloropropane (Rasche et al, 1990). It is surprising, however, that AMO in N. oceanus is inhibited by 4 mM 2-chloro-6trichloromethylpyridine (Salvas and Taylor, 1984;Ward, 1987), as is MMO activity in M. trichosporium OB3b and M. capsulatus Bath (Topp and Knowles, 1982).…”

Section: Differential Inhibition Of Monooxygenases By Phenyacetylene 489supporting

confidence: 70%

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Lontoh

DiSpirito

Krema

et al. 2000

Environmental Microbiology

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Phenylacetylene was investigated as a differential inhibitor of ammonia monooxygenase (AMO), soluble methane monooxygenase (sMMO) and membrane-associated or particulate methane monooxygenase (pMMO) in vivo. At phenylacetylene concentrations > 1 microM, whole-cell AMO activity in Nitrosomonas europaea was completely inhibited. Phenylacetylene concentrations above 100 microM inhibited more than 90% of sMMO activity in Methylococcus capsulatus Bath and Methylosinus trichosporium OB3b. In contrast, activity of pMMO in M. trichosporium OB3b, M. capsulatus Bath, Methylomicrobium album BG8, Methylobacter marinus A45 and Methylomonas strain MN was still measurable at phenylacetylene concentrations up to 1,000 microM. AMO of Nitrosococcus oceanus has more sequence similarity to pMMO than to AMO of N. europaea. Correspondingly, AMO in N. oceanus was also measurable in the presence of 1,000 microM phenylacetylene. Measurement of oxygen uptake indicated that phenylacetylene acted as a specific and mechanistic-based inhibitor of whole-cell sMMO activity; inactivation of sMMO was irreversible, time dependent, first order and required catalytic turnover. Corresponding measurement of oxygen uptake in whole cells of methanotrophs expressing pMMO showed that pMMO activity was inhibited by phenylacetylene, but only if methane was already being oxidized, and then only at much higher concentrations of phenylacetylene and at lower rates compared with sMMO. As phenylacetylene has a high solubility and low volatility, it may prove to be useful for monitoring methanotrophic and nitrifying activity as well as identifying the form of MMO predominantly expressed in situ.

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Section: Differential Inhibition Of Monooxygenases By Phenyacetylene 489supporting

confidence: 70%

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Lontoh

DiSpirito

Krema

et al. 2000

Environmental Microbiology

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“…This changing in nitritation performance was related to the production of imidazoles by the pharmaceutical company from day 64 on, and thus the confrontation of the microbial populations of the WWTPs with chemicals used in this production process, which are present in the influent. Imidazole production related products and used chlorinated and nonchlorinated solvents are well-known alternative substrates for ammonia and this way inhibit the growth of ammonia oxidizing bacteria (Rasche et al 1990;Keener and Arp 1994;McCarty 1999). They influence ammonium monooxygenase (AMO) activity by three distinct mechanisms: (i) direct binding and interaction with AMO, (ii) interference with the supply of reductant needed for monooxygenase activity, and (iii) the oxidation of substrates to give products that are highly reactive and inactivate AMO and/or other enzymes (Keener and Arp 1993;McCarty 1999).…”

Section: Discussionmentioning

confidence: 99%

Failure of the ammonia oxidation process in two pharmaceutical wastewater treatment plants is linked to shifts in the bacterial communities

et al. 2005

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Aims: To investigate whether two different wastewater treatment plants (WWTPs) -treating the same pharmaceutical influent -select for a different bacterial and/or ammonia oxidizing bacterial (AOB) community. Methods and Results: Molecular fingerprinting demonstrated that each WWTP had its own total bacterial and AOB community structure, but Nitrosomonas eutropha and N. europea were dominant in both WWTP A and B. The DNA and RNA analysis of the AOB communities revealed different patterns; so the most abundant species may not necessarily be the most active ones. Nitritation failures, monitored by chemical parameter analysis, were reflected as AOB community shifts and visualized by denaturing gradient gel electrophoresis (DGGE)-based moving window analysis. Conclusions: This research demonstrated the link between functional performance (nitritation parameters) and the presence and activity of a specific microbial ecology (AOB). Clustering and moving window analysis based on DGGE showed to be valuable to monitor community shifts in both WWTPs. Significance and Impact of the Study: This study of specific community shifts together with functional parameter analysis has potential as a tool for relating functional instability (such as operational failures) to specificbacterial community shifts.

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“…and fungicidal properties. Chloropicrin is toxic to mam-Bacteria have also been implicated in the oxidation mals, producing short-and long-term effects, and was of MeBr (Rasche et al, 1990;Oremland et al, 1994; used as a war gas during World War I. The mode of Miller et al, 1997;Ou et al, 1997).…”

Section: Methyl Bromidementioning

confidence: 99%

“…Degradation of CP in soil follows first-order kinetics Rasche et al (1990) found that two soil ammonia- (Gan et al, 2000a). In Arlington sandy loam (coarseoxidizing nitrifiers, Nitrosomonas europaea and Nitroloamy, mixed, active, thermic Haplic Durixeralf), Carsisolobus multiformis, consumed MeBr only in the prestas loamy sand (mixed, hyperthermic Typic Torripsamence of ammonium chloride.…”

Section: Methyl Bromidementioning

confidence: 99%

Degradation of Fumigant Pesticides: 1,3-Dichloropropene, Methyl Isothiocyanate, Chloropicrin, and Methyl Bromide

Dungan¹,

Yates

2003

Vadose Zone Journal

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where C is chemical concentration (mg kg 1) at time t (h), C o is initial concentration (mg kg 1), and k 1 is the Soil fumigation using shank injection creates high fumigant concen-first-order rate constant (h 1) independent of C and C o. tration gradients in soil from the injection point to the soil surface. A temperature gradient also exists along the soil profile. We studied In deriving Eq. [2], it is assumed that only pesticide the degradation of methyl isothiocyanate (MITC) and 1,3-dichloro-concentration changes during the course of observation; propene (1,3-D) in an Arlington sandy loam (coarse-loamy, mixed, concentrations of other species, such as degrading mi-thermic Haplic Durixeralf) at four temperatures and four initial con-croorganisms, remain unchanged. A general equation centrations. We then tested the applicability of first-order, half-order, for nth-order kinetics (n 1) of this type is given by and second-order kinetics, and the Michaelis-Menten model for de-(Sparks, 1989): scribing fumigant degradation as affected by temperature and initial concentration. Overall, none of the models adequately described the 1 n 1 (1 C n1 1 C o n1) k n t [3] degradation of MITC and 1,3-D isomers over the range of the initial concentrations. First-order and half-order kinetics adequately described the degradation of MITC and 1,3-D isomers at each initial where k n is the rate constant for nth-order kinetics, concentration, with the correlation coefficients greater than 0.78 (r 2 independent of C and C o. For example, for second-order 0.78). However, the derived rate constant was dependent on the initial kinetics (n 2), plotting 1/C with respect to time (t) concentration. The first-order rate constants varied between 6 and should yield a straight line. 10 for MITC for the concentration range of 3 to 140 mg kg 1 , and between 1.5 and 4 for 1,3-D isomers for the concentration range of For most pesticides used in agricultural systems, the 0.6 to 60 mg kg 1 , depending on temperature. For the same initial effect of concentration on pesticide degradation may be concentration range, the variation in the half-order rate constants was reasonably approximated by pseudo first-order kinetics, between 1.4 and 1.7 for MITC and between 3.1 and 6.1 for 1,3-D as these pesticides are applied at rates from less than 1 isomers, depending on temperature. Second-order kinetics and the kg ha 1 to a few kg ha 1 (Grover et al., 1997; Wolt, Michaelis-Menten model did not satisfactorily describe the degrada-1997; Kearney and Wauchope, 1998). However, several tion at all initial concentrations. The degradation of MITC and 1,3-D studies have shown that even in a well-controlled envi-was primarily biodegradation, which was affected by temperature ronment, degradation of pesticides in soils did not al-between 20 and 40C, following the Arrhenius equation (r 2 0.74). ways follow first-order kinetics (Hamaker et al., 1968; Hance and McKone, 1971; Helweg, 1975; Reffstrup et al., 1998). A difficulty commonly encountered is that A ccurate assessments...

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Biodegradation of Halogenated Hydrocarbon Fumigants by Nitrifying Bacteria

Cited by 87 publications

References 15 publications

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Failure of the ammonia oxidation process in two pharmaceutical wastewater treatment plants is linked to shifts in the bacterial communities

Degradation of Fumigant Pesticides: 1,3-Dichloropropene, Methyl Isothiocyanate, Chloropicrin, and Methyl Bromide

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