Biodegradation of high-molecular-mass heteroatomic components of heavy oil

Kopytov, M. A.; Filatov, Dmitry A.; Алтунина, Л. К.

doi:10.1134/s0965544113040087

Cited by 12 publications

(3 citation statements)

References 4 publications

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“…Sequence alignment of the three sequences per bacterial strain was performed using the maximum likelihood method of Hasegawa–Kishino–Yano model [ 13 ].The aligned sequences of the bacterial strains were submitted to GenBank NCBI. The tree with the highest log likelihood was −2378.5282.…”

Section: Methodsmentioning

confidence: 99%

Microbial enhanced heavy crude oil recovery through biodegradation using bacterial isolates from an Omani oil field

et al. 2015

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BackgroundBiodegradation is a cheap and environmentally friendly process that could breakdown and utilizes heavy crude oil (HCO) resources. Numerous bacteria are able to grow using hydrocarbons as a carbon source; however, bacteria that are able to grow using HCO hydrocarbons are limited. In this study, HCO degrading bacteria were isolated from an Omani heavy crude oil field. They were then identified and assessed for their biodegradation and biotransformation abilities under aerobic and anaerobic conditions.ResultsBacteria were grown in five different minimum salts media. The isolates were identified by MALDI biotyper and 16S rRNA sequencing. The nucleotide sequences were submitted to GenBank (NCBI) database. The bacteria were identified as Bacillus subtilis and B. licheniformis. To assess microbial growth and biodegradation of HCO by well-assay on agar plates, samples were collected at different intervals. The HCO biodegradation and biotransformation were determined using GC-FID, which showed direct correlation of microbial growth with an increased biotransformation of light hydrocarbons (C12 and C14). Among the isolates, B. licheniformis AS5 was the most efficient isolate in biodegradation and biotransformation of the HCO. Therefore, isolate AS5 was used for heavy crude oil recovery experiments, in core flooding experiments using Berea core plugs, where an additional 16 % of oil initially in place was recovered.ConclusionsThis is the first report from Oman for bacteria isolated from an oil field that were able to degrade and transform HCO to lighter components, illustrating the potential use in HCO recovery. The data suggested that biodegradation and biotransformation processes may lead to additional oil recovery from heavy oil fields, if bacteria are grown in suitable medium under optimum growth conditions.

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Section: Methodsmentioning

confidence: 99%

Microbial enhanced heavy crude oil recovery through biodegradation using bacterial isolates from an Omani oil field

et al. 2015

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“…Unlike n-alkanes, highly branched alkanes, cycloalkanes, 4 to 6 ring condensed aromatics, alkylated thiophenes and dibenzothiophenes are harder to biodegrade and are partially metabolized or are completely recalcitrant [42]. The reactivity PAHs decreases in the following order: n-alkanes, Isoalkanes, cycloalkanes then aromatics [62]. Rhodococcus erythropolis bacteria that could biotransform PAHs, polychlorinated biphenyls and dibenzothiophenes [70].…”

Section: Bacillus Licheniformis and Bacillus Subtilismentioning

confidence: 99%

Bioremediation of Heavy Crude Oil Contamination

Al-Sayegh

Al-Wahaibi

Joshi³

et al. 2016

TOBIOTJ

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Crude oil contamination is one of the major environmental concerns and it has drawn interest from researchers and industries. Heavy oils contain 24-64% saturates and aromatics, 14-39% resins and 11-45% asphaltene. Resins and asphaltenes mainly consist of naphthenic aromatic hydrocarbons with alicyclic chains which are the hardest to degrade. Crude oil biodegradation process, with its minimal energy need and environmentally friendly approach, presents an opportunity for bioremediation and as well for enhanced oil recovery to utilize heavy oil resources in an efficient manner. Biodegradation entails crude oil utilization as a carbon source for microorganisms that in turn change the physical properties of heavy crude oil by oxidizing aromatic rings, chelating metals and severing internal bonds/chains between molecules. Biodegradation does not necessarily lower quality of crude oil as there are cases where quality was improved. This paper provides information on heavy crude oil chemistry, bioremediation concept, biodegradation enzymes, cases of Microbial Enhanced heavy crude Oil Recovery (MEOR) and screening criteria towards a better understanding of the biodegradation application. Through the utilization of single microorganisms and consortia, researchers were able to biodegrade single pure hydrocarbon components, transform heavy crude oil fractions to lighter fractions, remove heavy metals and reduce viscosity of crude oil.

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“…Впервые участие асфальтенов в процессах микробиологического окисления было показано в работе [5]. Результаты последних исследований [6,7] подтверждают способность микро-организмов окислять высокомолекулярные компоненты. Основная характеристика изменений асфальтенов получена на уровне структурно-группового анализа.…”

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Change in the Structure of Asphaltene Macromolecules of the Krapivinskoye Oil Field During Biological Oxidation

Cheshkova

Sagachenko

Min

et al. 2021

Journal of Siberian Federal University. Chemistry

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Using physico-chemical methods of research (elemental analysis, infrared spectroscopy, selective chemical destruction of sulfide and ester bonds, chromatomass spectrometry) the influence of biodegradation processes on the composition and structure of asphaltenes of light oil at the Krapivinskoye deposit was studied. The results of comparative characteristics of initial asphaltenes and asphaltenes after biodestruction are presented. Attention is paid to studying their structural parameters and composition of fragments bound in asphaltene molecules through ester and sulfide bridges. It has been shown that microbial oxidation of asphaltenes of light oil by aboriginal soil microflora (laboratory experiment) occurs through a series of catalytic processes with formation of intermediate products of transformation – alcohols, aldehydes, ketones and fatty acids. It has been established that “grey and ether-bound” fragments in asphaltene molecules of biodegradable oil differ from “bound” compounds in the structure of the original asphaltenes with the qualitative composition of saturated and aromatic hydrocarbons and heteroatomic components

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Biodegradation of high-molecular-mass heteroatomic components of heavy oil

Cited by 12 publications

References 4 publications

Microbial enhanced heavy crude oil recovery through biodegradation using bacterial isolates from an Omani oil field

Microbial enhanced heavy crude oil recovery through biodegradation using bacterial isolates from an Omani oil field

Bioremediation of Heavy Crude Oil Contamination

Change in the Structure of Asphaltene Macromolecules of the Krapivinskoye Oil Field During Biological Oxidation

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