Biodegradation of<i>N-methylated</i>carbamates by free and immobilized cells of newly isolated strain<i>Enterobacter cloacae</i>strain TA7

Fareed, Anum; Zaffar, Habiba; Rashid, Abbas; Shah, Muhammad Shakil; Naqvi, Tatheer Alam

doi:10.1080/10889868.2017.1404964

Cited by 31 publications

(17 citation statements)

References 22 publications

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“…Similar results were reported by Fareed et al [ 15 ], where immobilized cells of Enterobacter cloacae strain TA7 effectively degraded in N -methyl carbamates pesticides more rapidly than freely suspended cells counterpart. At 300 mg/L of phenol, the freely suspended cells showed a lower specific degradation rate, whereas, the immobilized cells demonstrated a higher phenol degradation rate [ 22 ].…”

Section: Resultssupporting

confidence: 90%

“…Degradation of pesticides, such as carbofuran, using free cells has been a challenge due to some limitations in their application for bioremediation, which includes cell separation, substrate inhibition, and difficulty in isolating a strain that can withstand high concentration of carbofuran and possible degradation within a shortest possible time. Therefore, to overcome these challenges, immobilized cell technology can be applied for the long-term stabilization of different pollutant-degrading microbes [ 13 , 14 , 15 ]. One of the most important aspects of bioremediation is maintaining high biomass of bacterial populations and increase the survival and retention of the bioremediation agents in the polluted areas.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

et al. 2020

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Extensive use of carbofuran insecticide harms the environment and human health. Carbofuran is an endocrine disruptor and has the highest acute toxicity to humans than all groups of carbamate pesticides used. Carbofuran is highly mobile in soil and soluble in water with a lengthy half-life (50 days). Therefore, it has the potential to contaminate groundwater and nearby water bodies after rainfall events. A bacterial strain BRC05 was isolated from agricultural soil characterized and presumptively identified as Enterobacter sp. The strain was immobilized using gellan gum as an entrapment material. The effect of different heavy metals and the ability of the immobilized cells to degrade carbofuran were compared with their free cell counterparts. The results showed a significant increase in the degradation of carbofuran by immobilized cells compared with freely suspended cells. Carbofuran was completely degraded within 9 h by immobilized cells at 50 mg/L, while it took 12 h for free cells to degrade carbofuran at the same concentration. Besides, the immobilized cells completely degraded carbofuran within 38 h at 100 mg/L. On the other hand, free cells degraded the compound in 68 h. The viability of the freely suspended cell and degradation efficiency was inhibited at a concentration greater than 100 mg/L. Whereas, the immobilized cells almost completely degraded carbofuran at 100 mg/L. At 250 mg/L concentration, the rate of degradation decreased significantly in free cells. The immobilized cells could also be reused for about nine cycles without losing their degradation activity. Hence, the gellan gum-immobilized cells of Enterobacter sp. could be potentially used in the bioremediation of carbofuran in contaminated soil.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

et al. 2020

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“…Previous studies have found that an enzymatic degradation system is more effective than the direct use of micro-organisms [95][96][97][98][99][100][101]. Genes and enzymes involved in the development of drugs have been investigated [102][103][104]. However, there are only a few studies on the enzymatic degradation pathway of methomyl.…”

Section: Molecular Mechanism Of Methomyl Degradationmentioning

confidence: 99%

Current Approaches to and Future Perspectives on Methomyl Degradation in Contaminated Soil/Water Environments

et al. 2020

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Methomyl is a broad-spectrum oxime carbamate commonly used to control arthropods, nematodes, flies, and crop pests. However, extensive use of this pesticide in agricultural practices has led to environmental toxicity and human health issues. Oxidation, incineration, adsorption, and microbial degradation methods have been developed to remove insecticidal residues from soil/water environments. Compared with physicochemical methods, biodegradation is considered to be a cost-effective and ecofriendly approach to the removal of pesticide residues. Therefore, micro-organisms have become a key component of the degradation and detoxification of methomyl through catabolic pathways and genetic determinants. Several species of methomyl-degrading bacteria have been isolated and characterized, including Paracoccus, Pseudomonas, Aminobacter, Flavobacterium, Alcaligenes, Bacillus, Serratia, Novosphingobium, and Trametes. The degradation pathways of methomyl and the fate of several metabolites have been investigated. Further in-depth studies based on molecular biology and genetics are needed to elaborate their role in the evolution of novel catabolic pathways and the microbial degradation of methomyl. In this review, we highlight the mechanism of microbial degradation of methomyl along with metabolic pathways and genes/enzymes of different genera.

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“…The genetically engineered microbes have substantial capacity to degrade the pesticide as they discharge several specific enzymes that have the specific catabolic gene into plasmids (Chen and Huang, 2016). The studies of recombinant DNA provide the means to develop DNA and RNA examinations for the purpose of identifying microorganisms from diverse populations with exceptional capacity to degrade pesticides (Ahmad, 2010) 2,4-D(2,4-dichloro-phenoxy acetic acid) Pseudomonas fluorescens Alginate beads (Ahmad, 2010;Boivin, Amellal, Schiavon and van Genuchten, 2005) Aldicarb Enterobactr cloacae Agar entrapment (Fareed et al, 2017) Carbofuran Enterobacter cloacae Agar entrapment (Fareed et al, 2017) Carbaryl Enterobacter cloacae Agar entrapment (Fareed et al, 2017…”

Section: Methyl Parathionmentioning

confidence: 99%

Soil Microorganisms and Their Potential in Pesticide Biodegradation; A Review Article

Mustapha

Halimoon

Johari

et al. 2018

Journal of Sustainable Agricultural Sciences

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P ESTICIDES play an important role in preventing insect pests and weeds in crops. However, excessive use of pesticides has been known to be unsafe, due to their toxicity to non-target organisms and the ecosystem. Biodegradation is an innovative approach for decontaminating pesticide pollution. However, compared with the list of extensively used pesticides there are few well-characterized strains of microbes that transform pesticides into less-toxic or more labile products at environmentally useful rates. Fortunately, the technology required to isolate and characterize such microbial strains has improved immensely in the recent years. Furthermore, recent experimental developments have made practical the modification of potentially beneficial biodegradation genes so that they may be optimally expressed in a wide range of microbial species. This reviews article explore the recent studies that have focused on biodegradation of pesticide residues, the mechanism of microbial degradation of pesticides, the factors that affect the degradation of pesticides and the new application of microbial degradation of pesticides.

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Biodegradation ofN-methylatedcarbamates by free and immobilized cells of newly isolated strainEnterobacter cloacaestrain TA7

Cited by 31 publications

References 22 publications

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

Current Approaches to and Future Perspectives on Methomyl Degradation in Contaminated Soil/Water Environments

Soil Microorganisms and Their Potential in Pesticide Biodegradation; A Review Article

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