Biodegradation of lignin and the associated degradation pathway by psychrotrophic Arthrobacter sp. C2 from the cold region of China

Jiang, Cheng; Cheng, Yi; Zang, Hailian; Chen, Xi; Wang, Yue; Zhang, Yuting; Wang, Jinming; Shen, Xiaohui; Li, Chunyan

doi:10.1007/s10570-019-02858-3

Cited by 46 publications

(26 citation statements)

References 68 publications

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“…At 9 days, only 2-methoxy-4-propylphenol was detected. The trend of the DyP expression level was consistent with that of enzyme activity (our previous study) ( Jiang et al, 2019 ). According to the single-factor experiment results ( Jiang et al, 2019 ), LiP activity and MnP activity showed a trend of first increasing and then decreasing, and the highest activities were observed at 7 days.…”

Section: Resultssupporting

confidence: 89%

“…Finally, the sample was filtered through a 0.22-μm filter membrane for GC–MS analysis. The details of the assay were described by Jiang et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…The strains were inoculated with a 2% inoculum size (OD 600 nm = 1) in 100 mL of MSM containing 3 g/L sodium lignin sulfonate and incubated for 9 days at 15°C. The degradation rate, bacterial growth and enzyme activities were measured at 1-day intervals as described by Jiang et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

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Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

et al. 2022

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In the cold regions of China, lignin-rich corn straw accumulates at high levels due to low temperatures. The application of psychrotrophic lignin-degrading bacteria should be an effective means of overcoming the low-temperature limit for lignin degradation and promoting the utilization of corn straw. However, this application is limited by the lack of suitable strains for decomposition of lignin; furthermore, the metabolic mechanism of psychrotrophic lignin-degrading bacteria is unclear. Here, the whole genome of the psychrotrophic lignin-degrading bacterium Arthrobacter sp. C2, isolated in our previous work, was sequenced. Comparative genomics revealed that C2 contained unique genes related to lignin degradation and low-temperature adaptability. DyP may participate in lignin degradation and may be a cold-adapted enzyme. Moreover, DyP was proven to catalyze lignin Cα-Cβ bond cleavage. Deletion and complementation of the DyP gene verified its ability to catalyze the first-step reaction of lignin degradation. Comparative transcriptomic analysis revealed that the transcriptional expression of the DyP gene was upregulated, and the genetic compensation mechanism allowed C2ΔDyP to degrade lignin, which provided novel insights into the survival strategy of the psychrotrophic mutant strain C2ΔdyP. This study improved our understanding of the metabolic mechanism of psychrotrophic lignin-degrading bacteria and provided potential application options for energy-saving production using cold-adapted lignin-degrading enzymes.

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Section: Resultssupporting

confidence: 89%

“…Finally, the sample was filtered through a 0.22-μm filter membrane for GC–MS analysis. The details of the assay were described by Jiang et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

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Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

et al. 2022

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“…B-9 Kraft lignin 45% [ 61 ] (Chai et al) Bacillus ligniniphilus L1 Alkaline lignin 38.9% [ 62 ] (Zhu et al) B. amyloliquefaciens SL-7 Tobacco straw lignin 28.55% [ 63 ] (Mei et al) Facultative anaerobe bacteria Enterobacter lignolyticus SCF1 Alkali lignin 56% [ 68 ] (DeAngelis et al) Acetoanaerobium sp Kraft lignin 24.9% [ 71 ] (Duan et al) Extremophile bacteria Caldicellulosiruptor kronotskyensis Natural rice straw 52.5% [ 74 ] (Peng et al) Arthrobacter sp. C2 Sodium lignin sulfonate 40.1% [ 75 ] (Jiang et al) …”

Section: Lignin Depolymerization By Microorganisms and Enzymesmentioning

confidence: 99%

“…Besides, lignin degradation was also detected by a psychrotrophic bacteria Arthrobacter sp. C2 at low temperature, and the intermediates of acids, phenols, aldehydes, and alcohols were identified after the treatment [ 75 ].…”

Section: Lignin Depolymerization By Microorganisms and Enzymesmentioning

confidence: 99%

Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis

Weng

Peng

Han

2021

Biotechnol Biofuels

165

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Lignin, the most abundant renewable aromatic compound in nature, is an excellent feedstock for value-added bioproducts manufacturing; while the intrinsic heterogeneity and recalcitrance of which hindered the efficient lignin biorefinery and utilization. Compared with chemical processing, bioprocessing with microbial and enzymatic catalysis is a clean and efficient method for lignin depolymerization and conversion. Generally, lignin bioprocessing involves lignin decomposition to lignin-based aromatics via extracellular microbial enzymes and further converted to value-added bioproducts through microbial metabolism. In the review, the most recent advances in degradation and conversion of lignin to value-added bioproducts catalyzed by microbes and enzymes were summarized. The lignin-degrading microorganisms of white-rot fungi, brown-rot fungi, soft-rot fungi, and bacteria under aerobic and anaerobic conditions were comparatively analyzed. The catalytic metabolism of the microbial lignin-degrading enzymes of laccase, lignin peroxidase, manganese peroxidase, biphenyl bond cleavage enzyme, versatile peroxidase, and β-etherize was discussed. The microbial metabolic process of H-lignin, G-lignin, S-lignin based derivatives, protocatechuic acid, and catechol was reviewed. Lignin was depolymerized to lignin-derived aromatic compounds by the secreted enzymes of fungi and bacteria, and the aromatics were converted to value-added compounds through microbial catalysis and metabolic engineering. The review also proposes new insights for future work to overcome the recalcitrance of lignin and convert it to value-added bioproducts by microbial and enzymatic catalysis.

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Lignin Depolymerization Technologies

2023

Depolymerization of Lignin to Produce Value Added Chemicals

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Biodegradation of lignin and the associated degradation pathway by psychrotrophic Arthrobacter sp. C2 from the cold region of China

Cited by 46 publications

References 68 publications

Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis

Lignin Depolymerization Technologies

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