Biodegradation of low-density polyethylene (LDPE) using the mixed culture of Aspergillus carbonarius and A. fumigates

El-Sayed, Manal Tawfeek; Rabie, Gamal H.; Hamed, Esraa A.

doi:10.1007/s10668-021-01258-7

Cited by 30 publications

(11 citation statements)

References 67 publications

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“…Significant weight loss (%) of 15.12% was observed in C. sphaerospermum treated LDPE film whereas there was no change in weight was observed in control. El-Sayed et al 2 reported 3.8%, 2.26% weight loss by Aspergillus carbonarius and A. fumigatus respectively on 30 days whereas mixed culture showed a 5.85% weight loss under the same condition. Nearly 35% and 38% weight loss in 90 days by A. clavatus and Penicillium citrinuum respectively was reported 34 , 35 .…”

Section: Resultsmentioning

confidence: 96%

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Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Sathiyabama,

Boomija,

Sathiyamoorthy

et al. 2024

Sci Rep

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Plastic accumulation is a severe threat to the environment due to its resistivity to thermal, mechanical and biological processes. In recent years, microbial degradation of plastic waste disposal is of interest because of its eco-friendly nature. In this study, a total of 33 fungi were isolated from the plastisphere and out of which 28 fungal species showed halo zone of clearance in agarized LDPE media. The fungus showing highest zone of clearance was further used to evaluate its degradation potential. Based on morphological and molecular technique, the fungus was identified as Cladosporium sphaerospermum. The biodegradation of LDPE by C. sphaerospermum was evaluated by various methods. The exposure of LDPE with C. sphaerospermum resulted in weight loss (15.23%) in seven days, higher reduction rate (0.0224/day) and lower half-life (30.93 days). FTIR analysis showed changes in functional group and increased carbonyl index in LDPE treated with C. sphaerospermum. SEMimages evidenced the formation of pits, surface aberrations and grooves on the LDPE film treated with the fungus whereas the untreated control LDPE film showed no change. AFM analysis confirmed the surface changes and roughness in fungus treated LDPE film. This might be due to the extracellular lignolytic enzymes secreted by C. sphaerospermum grown on LDPE. The degradation of polyethylene by Short chain alkanes such as dodecane, hexasiloxane and silane were identified in the extract of fungus incubated with LDPE film through GC–MS analysis which might be due to the degradation of LDPE film by C. sphaerospermum. This was the first report on the LDPE degradation by C. sphaerospermum in very short duration which enables green scavenging of plastic wastes.

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Section: Resultsmentioning

confidence: 96%

“…Plastics have become an inevitable material in our lives, and it plays a significant role in the global economy 1 . Plastic production increased over time, and approximately three hundred and twenty million tons of plastics are produced every year 2 , 3 . Polyethylene is the prominent plastic widely used in various industries.…”

Section: Introductionmentioning

confidence: 99%

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Sathiyabama,

Boomija,

Sathiyamoorthy

et al. 2024

Sci Rep

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“…A. ibericus showed a 99 % similarity with Aspergillus carbonarius (Figure 1). Aspergillus carbonarius have been previously implicated in production of invertase and β-D-fructosyltransferase [33], biodegradation of polyethylene [34] and hydrocarbons [35]. Species of Aspergillus are versatile and majority of them have been documented to play a significant role in lignocellulosic degradation through secretion of extracellular enzymes.…”

Section: Molecular Analysis and Phylogenymentioning

confidence: 99%

Optimizing Laccase Production from Delonix regia Pods by Aspergillus carbonarius F5 Using Response Surface Methodology and it’s Dye Decolorization Potential

Arekemase

Tiamiyu

Kazeem

et al. 2022

AJB2T

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Introduction: Laccase is a muilti-copper enzyme that directs oxidation of compounds by reducing oxygen to water molecules. It has been identified as a significant enzyme which possesses diverse interest in industrial sector due to its ability to degrade toxicants from dye-utilizing related industries. Aim: The current research focuses on the optimization of laccase from Aspergillus carbonarius F5 on Delonix regia pods, for application in dye degradation. Methodology: Potential laccase producing fungi were screened and characterized using 18s rRNA. Laccase production was further conducted on untreated and acid pretreated Delonix regia pods. Plackett-Burman design and Central Composite Design (CCD) of the Response Surface Methodology (RSM) was used to screen for various nutritional and environmental factors to improve the yield of the enzyme and then applied for dye decolorization studies. Results: The fungal strain with the highest laccase activity was identified as Aspergillus carbonarius F5. The acid pretreated Delonix regia pods showed considerably high enzyme activity when compared with untreated. Moreover, variation of notable nutritional and environmental factors were detected to improve laccase yield. The effect of input parameters such as incubation period, pH, temperature and MgSO4 were documented as the major factors influencing high laccase yield using Plackett-Burman design. Results obtained from the modelling of experiment using CCD-RSM shows that maximum laccase production of 8.04 U/ml was recorded at temperature 36°C, pH 6, MgSO4.7H2O at 0.2 (g/L), and 7 days which reveals a 8.28 –fold increase. The outcome of the investigation performed on dye decolorization suggests an 87.6 % and 62.6 % degradation on congo red and malachite green dyes respectively. Conclusion: Delonix regia pods could be an added substrate, suitable for optimization of laccase by Aspergillus carbonarius F5 and the enzyme was capable of decolorizing industrial dyes.

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“…Furthermore, the biodegradation of highly durable polymers such as low-density polyethylene (LDPE) is less suitable for microorganisms due to its higher redox potential, requiring more energy to break the C-C bonds. The worldwide utilization of polyethylene rapidly increases by over 320 million tons every year and seems to be doubled by 2034 [2]. The pollution caused by polyethylene includes blocking drains, water, soil, air contamination, sewage lines around the urban areas due to nondegradability and is not beneficial to the topsoil [3].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation and detoxification of low-density polyethylene by an indigenous strain Bacillus licheniformis SARR1

Rani¹,

Rathee²,

Kumari³

et al. 2022

J Appl Biol Biotech

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Plastics are synthetic polymers, which are frequently used in daily life for a wide range of purposes. These plastic wastes are accumulated and generate plastic pollution in the environment. It needs many years for complete deterioration in the environmental conditions. Biodegradation is the most promising method to treat plastic pollution, as the microorganisms utilize the low-density polyethylene (LDPE) as a sole source of carbon, and this indicates an innovative approach to manage the problem of plastic waste. The isolate SARR1 was identified as Bacillus licheniformis using the National Center for Biotechnology Information (NCBI) database. The isolate SARR1 showed an LDPE removal rate (K) of 0.069 g day −1 with a half-life of approximately 335.32 days to degrade LDPE strips. The biomass production was 0.98 ± 0.006 gl −1 (X m ) during the incubation of 30 days, and the percentage of crystallinity was significantly decreased from 71.69% to 50.78% due to biodegradation. The esterase and lipase activity of isolate SARR1 was studied using UV visible spectroscopy. The gas chromatography-mass spectrometry analysis confirmed the synthesis of acetone, diazene dimethyl, and carbamimidothioic acid, 1-methylethyl ester with different peak area percentages of 23.38%, 65.58%, and 11.04%, respectively. Seed germination study showed that the compounds formed after biodegradation of LDPE by bacterial strain SARR1 were eco-friendly.

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Biodegradation of low-density polyethylene (LDPE) using the mixed culture of Aspergillus carbonarius and A. fumigates

Cited by 30 publications

References 67 publications

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Mycodegradation of low-density polyethylene by Cladosporium sphaerospermum, isolated from platisphere

Optimizing Laccase Production from Delonix regia Pods by Aspergillus carbonarius F5 Using Response Surface Methodology and it’s Dye Decolorization Potential

Biodegradation and detoxification of low-density polyethylene by an indigenous strain Bacillus licheniformis SARR1

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