Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Alfonso-Gordillo, Guadalupe; Flores-Ortíz, César Mateo; Morales-Barrera, Liliana; Cristiani‐Urbina, Eliseo

doi:10.1371/journal.pone.0167494

Cited by 20 publications

(12 citation statements)

References 57 publications

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“…Notably, the packed-bed reactor system was able to accelerate the degradation of ametoctradin. Packed-bed bioreactors have been used for decades for microbial biodegradation of environmental contaminants and compounds of interest, as well as for the production of value-added products such as ethanol and lactic acid ( Shiotani and Yamané, 1981 ; Strandberg et al, 1989 ; Bruno-Bárcena et al, 1999 ; Alfonso-Gordillo et al, 2016 ; Geed et al, 2017 ; Mavriou et al, 2020 ). It has been shown previously that sessile microbial cells are more resistant to toxic substances than planktonic cells, making packed-bed bioreactors attractive for studying the degradation of xenobiotic compounds ( Parkin and Speece, 1983 ).…”

Section: Discussionmentioning

confidence: 99%

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Whittington

Singh

et al. 2020

Front. Microbiol.

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Pesticide-resistant plant pathogens are an increasing threat to the global food supply and have generated a need for novel, efficacious agrochemicals. The current regulatory process for approving new agrochemicals is a tedious but necessary process. One way to accelerate the safety evaluation process is to utilize in vitro systems to demonstrate pesticide degradation by soil microbes prior to ex vivo soil evaluations. This approach may have the capability to generate metabolic profiles free of inhibitory substances, such as humic acids, commonly present in ex vivo soil systems. In this study, we used a packed-bed microbial bioreactor to assess the role of the natural soil microbial community during biodegradation of the triazolopyrimidine fungicide, ametoctradin. Metabolite profiles produced during in vitro ametoctradin degradation were similar to the metabolite profiles obtained during environmental fate studies and demonstrated the degradation of 81% of the parent compound in 72 h compared to a half-life of 2 weeks when ametoctradin was left in the soil. The microbial communities of four different soil locations and the bioreactor microbiome were compared using high throughput sequencing. It was found that biodegradation of ametoctradin in both ex vivo soils and in vitro in the bioreactor correlated with an increase in the relative abundance of Burkholderiales, well characterized microbial degraders of xenobiotic compounds.

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Section: Discussionmentioning

confidence: 99%

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Whittington

Singh

et al. 2020

Front. Microbiol.

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“…The separation of S. novella R8b extract (265.6 mg) yielded 14 different and homogeneous fractions. The main secondary metabolite of S. novella R8b was purified by the preparative TLC of fractions (2-3), (3-5)III and (6)(7)(8)(9)(10)(11)(12)(13)(14)d. The metabolite isolated showed chromatographic and spectroscopic properties (NMR and MS) similar to those reported in literature [26] for a compound known as maculosin (Fig 6).…”

Section: Bacterial Biocontrol Activitymentioning

confidence: 99%

“…The development of technologies to treat MtBE-contaminated soil is of great importance worldwide. Different remediation technologies, such as soil flushing, soil washing, air stripping, adsorption, oxidation, phytoremediation, biodegradation processes and much more have been proposed [4,7]. Among these strategies, biodegradation processes are recognized as innovative, cost-effective and environmentally friendly options for the detoxification of MtBE-contaminated soil [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Different remediation technologies, such as soil flushing, soil washing, air stripping, adsorption, oxidation, phytoremediation, biodegradation processes and much more have been proposed [4,7]. Among these strategies, biodegradation processes are recognized as innovative, cost-effective and environmentally friendly options for the detoxification of MtBE-contaminated soil [4,7,8]. Some microorganisms can partially or completely degrade MtBE under aerobic or anaerobic conditions [9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

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Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

et al. 2020

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A total of fifteen potential methyl t-butyl ether (MtBE)-degrading bacterial strains were isolated from contaminated soil. They have been identified as belonging to the genera Bacillus, Pseudomonas, Kocuria, Janibacter, Starkeya, Bosea, Mycolicibacterium, and Rhodovarius. Bacillus aryabhattai R1B, S. novella R8b, and M. mucogenicum R8i were able to grow using MtBE as carbon source, exhibiting different growth behavior and contaminant degradation ability. Their biocontrol ability was tested against various fungal pathogens. Both S. novella R8b and B. aryabhattai were effective in reducing the development of necrotic areas on leaves within 48 hours from Botritys cinerea and Alternaria alternata inoculation. Whereas, M. mucogenicum effectively controlled B. cinerea after 72 hours. Similar results were achieved using Pythium ultimum, in which the application of isolated bacteria increased seed germination. Only M. mucogenicum elicited tomato plants resistance against B. cinerea. This is the first report describing the occurrence of bioremediation and biocontrol activities in M. mucogenicum, B. aryabhattai and S. novella species. The production of maculosin and its antibiotic activity against Rhizoctonia solani has been reported for first time from S. novella. Our results highlight the importance of multidisciplinary approaches to achieve a consistent selection of bacterial strains useful for plant protection and bioremediation purposes.

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“…In a study by Salanitro et al, biomass yields (gram of dry weight cells per gram of MTBE) were 0.21 to 0.28 (32). Some studies have also investigated MTBE biodegradation by Mycobacterium (33,34). But so far, no study has been conducted to investigate the role of K. planticola in bioremediation of MTBE, while some species of Klebsiella have been found to be capable of utilizing MTBE, n-hexadecane, and other hydrocarbons contaminating soil (20,35,36).…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of methyl tertiary-butyl ether (MTBE) by three pure bacterial cultures

Yousefi¹,

Tahernezhad²,

Mousavinasab³

et al. 2018

Environ Health Eng Manag

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Background: Bioremediation of groundwater and soil contamination is more economical than physicochemical remediation. The present study focused on the bioremediation capability of two bacterial species (Klebsiella planticola and Enterobacter cloacae) from the family Enterobacteriaceae. These bacteria have been identified as new species with capability of degrading methyl tertiary-butyl ether (MTBE). In order to enhance their degradation capability, selected concentrations and retention time were investigated. Methods: The bacteria were cultured on the nutrient agar (NA) medium at room temperature. pH of the medium was adjusted to 7. The medium was autoclaved at 121°C for 15 minutes and incubated for 24 hours at 35°C. After 24 hours, the mixture was inoculated into 50 mL of Luria Bertani (LB) liquid medium containing 50 and 150 ppm MTBE. The cultures were incubated for 2 and 5 days at 35°C and shacked on a shaker at 150 rpm. Cell concentrations of the bacteria in pure culture were determined from the optical density at 600 nm using a UV-VIS spectrophotometer. Then, the culture was centrifuged at 3800 rpm for 20 minutes. In the next step, the MTBE concentration in the supernatant was measured by gas chromatography/mass spectrometry (GC/MS, Agilent Technologies, 5975, US10304411, 5.02.07). Results:The results showed that both strains are able to grow in the presence of 50 and 150 ppm MTBE. In the best conditions, when cell density was 3×108 CFU/mL during 5 days, the highest rate of MTBE degradation for K. planticola and E. cloacae, was 43% and 40%, respectively. It was also revealed that Escherichia coli can degrade 50 and 150 ppm MTBE about 19.8% and 13.65%, respectively. Conclusion: It seems that E. coli can be a good candidate for MTBE degradation at high concentrations for a time longer than that in the present study. It was also found that the species have high performance at 50 ppm than 150 ppm. So, these bacteria can remove MTBE from the environment.

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Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Cited by 20 publications

References 57 publications

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

Bioremediation of methyl tertiary-butyl ether (MTBE) by three pure bacterial cultures

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