Biodegradation of microcystins by aquatic Burkholderia sp. from a South Brazilian coastal lagoon

Lemes, Gilmar Antonio Farias; Kersanach, Ralf; Pinto, Luciano da Silva; Dellagostin, Odir A.; Yúnes, João; Matthiensen, Alexandre

doi:10.1016/j.ecoenv.2007.03.013

Cited by 87 publications

(49 citation statements)

References 43 publications

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“…[11], Sphingomonas sp. [8,15,20,21,29], and Pseudomonas aeruginosa [22] for the MCs biodegradation were successfully isolated from the surface water or sediment of HCB lakes. Sphingomonas sp.…”

Section: Biodegradation Of Mc-lr By Sphingopyxis Sp Ustb-05mentioning

confidence: 99%

“…Sphingomonas sp. Y2 was reported to have the highest biodegradation rate of 5.4 mg$L -1 MC-LR per day [8,22,24]. A very promising bacterial strain isolated and identified as Sphingopyxis sp.…”

Section: Biodegradation Of Mc-lr By Sphingopyxis Sp Ustb-05mentioning

confidence: 99%

“…[8,15,20,21], Pseudomonas aeruginosa [23] Burkholderia sp. [22],and Paucibacter toxinivorans [26], isolated from Australia reservoirs, Japan lakes, a Brazilian coastal lagoon, and Finland lakes were found to have the capabilities of biodegrading MCs, respectively. Most of the strains reported belong to genus Sphingomonas, such as ACM-3962 [7], Y2 [8], LH21 [8], B-9 [15], 7CY [20], and CBA4 [21].…”

Section: Introductionmentioning

confidence: 99%

“…Aerobic biodegradation has been considered to be the main attenuation mechanism for MCs, and this process was reported to occur in diverse ecosystems, such as rivers [12], sand filters [11], reservoirs [20,21], coastal lagoons [22], lakes [17,23,24], and sewage effluent [25]. Bacterial strains of Sphingpoyxis sp.…”

Section: Introductionmentioning

confidence: 99%

“…Sphingomonas sp. Y2 was reported to have the highest degradation rate of 5.4 mg$L -1 per day for MC-LR [8,22,24]. On the other hand, Holst et al [27] and Chen et al [28] investigated the potential for anoxic biodegradation of microcystin and the effects of environmental factors on the process through a series of wellcontrolled microcosm experiments.…”

Section: Introductionmentioning

confidence: 99%

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Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Xiao

Yan

Wang

et al. 2010

Front. Environ. Sci. Eng. China

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A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as Sphingopyxis sp. USTB-05, which was the first isolated MCs-biodegrading Sphingopyxis sp. in China. The average biodegradation rate of MC-LR by Sphingopyxis sp. USTB-05 was 28.8 mg$L -1 per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30°C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg$L -1 in water was biodegraded by Sphingopyxis sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg$L -1 could be completely removed in 3 h by CE of Sphingopyxis sp. USTB-05 containing 350 mg$L -1 protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.

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Section: Biodegradation Of Mc-lr By Sphingopyxis Sp Ustb-05mentioning

confidence: 99%

Section: Biodegradation Of Mc-lr By Sphingopyxis Sp Ustb-05mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Xiao

Yan

Wang

et al. 2010

Front. Environ. Sci. Eng. China

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Biological Treatment for the Destruction of Cyanotoxins

Dziga

Nybom

Gągała

et al. 2020

Water Treatment for Purification From Cyanobacteria and Cyanotoxins

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The effects of Microcystis aeruginosa cells lysate containing microcystins on physiological and molecular responses in the nematode Caenorhabditis elegans

Sopezki

Josende

Cruz

et al. 2020

Environmental Toxicology

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Microcystins (MCs) are potent toxins produced by environmental cyanobacterial blooms. The present study evaluated the effects of a Microcystis aeruginosa cyanobacterial lysate containing 0.1, 1, and 10 μg L−1 MC‐LR equivalent in the C. elegans Bristol N2 wild‐type and the effects caused by equivalent concentrations of a MC‐LR standard. The lysate was prepared from a culture of toxic strain (RST9501) originated from the Patos Lagoon Estuary (RS, Brazil). The minimal concentration necessary to cause significant effects in C. elegans under exposure to M. aeruginosa lysate or to MC‐LR standard were, respectively, 10 and 0.1 μg L−1 MC‐LR equivalent for growth and 10 and 1 μg L−1 MC‐LR equivalent for fertility. Reproduction (ie, brood size) was only affected by the exposure to 10 μg L−1 MC‐LR standard and was not affected by the lysate. The nematodes that were exposed to lysate containing 1 μg L−1 MC‐LR equivalent or MC‐LR were also analyzed for pharyngeal pumping and gene expression using RT‐qPCR. The worms’ rhythmic contractions of the pharynx were similarly affected by the lysate containing 1 μg L−1 of MC‐LR equivalent and the MC‐LR standard. The MC‐LR standard caused down‐regulation of genes related to growth (daf‐16), fertility (spe‐10), and biotransformation (gst‐2). This is the first study to evaluate the effects of a toxic cyanobacterial lysate using the C. elegans model. This study suggests the organism as a potential biotest to evaluate toxicity of natural waters containing M. aeruginosa cells and to environmental risk assessment associated to cyanobacterial bloom events.

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Biodegradation of microcystins by aquatic Burkholderia sp. from a South Brazilian coastal lagoon

Cited by 87 publications

References 43 publications

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Biological Treatment for the Destruction of Cyanotoxins

The effects of Microcystis aeruginosa cells lysate containing microcystins on physiological and molecular responses in the nematode Caenorhabditis elegans

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