Biodegradation of monocrotophos by bacteria isolated from soil

Srinivasulu, M.; Nilanjan, P C; Chakravarthi, Balabhadrapatruni V. S. K.; Jayabaskaran, C; Jaffer, M G; Naga, R M; Manjunatha, B.; Darwin, R O; Juan, Ocampo-López; Rangaswamy, V.

doi:10.5897/ajb2015.14885

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…investigated cyclohexanone degradation using Pseudomonas with phenol production. The metabolite detected in GC MS during monocrotophos degradation in the present study is acetamide, N-acetyl N-methyl, which were also detected as an intermediate metabolite of monocrotophos degradation bySrinivasulu et al (2017) [18]. According toAcharya et al (2015) [3], N-methylacetoacetamide was degraded to methylamine and carbon dioxide and these products were detected during monocrotophos degradation using isolate B. subtilis KPA-1.…”

mentioning

confidence: 51%

“…Thus, degradation analysis revealed that consortium is more effective than pure bacterial isolates for monocrotophos degradation. Another study found that Rhodococcus phenolicus and R. ruber mediated degradation ranged from 30% to 45% after 96 h of incubation [18]. After 17 days of incubation, Sidhu et al (2015) [6] reported 67.8%, 16.58%, and 6.67% degradation using isolated P. synxantha, B. subtilis, and S. enterica.…”

Section: Monocrotophos Degradationmentioning

confidence: 98%

“…The pesticide degradation study was carried out in sterile MSM broth containing (per L) of of 1.5 g NH 4 NO 3 , 1.5 g K 2 HPO 4 , 0.5 g KH 2 PO 4 , 0.2 g MgSO 4 , 0.5 g NaCl, and pH 7.0 [18]. The filter-sterilized 500 ppm monocrotophos was added separately into the MSM broth as a sole carbon source.…”

Section: Monocrotophos Degradationmentioning

confidence: 99%

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Degradation and detoxification of monocrotophos using bacterial consortium

Jokhakar¹,

Dudhagara²

2022

J App Biol biotech

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The uncontrol use of organophosphate insecticide in agricultural fields to protect crop yields imposes severe environmental issues due to its long persistence in soil and water. For cotton, maize, and sugarcane, monocrotophos is a widely used and effective organophosphate pesticide. To lessen the harm to the environment, biodegradation and detoxification of pesticides need to be developed. In the present study, the polluted site Amlakhadi, Gujarat, India, was explored for the isolation of monocrotophos degrading bacteria. Gordonia terrae, Pseudomonas aeruginosa, Serratia surfactantfaciens, and a consortium of these three bacteria were chosen for further study on degradation after being tested for their tolerance to monocrotophos. Accelerated degradation by the consortium was confirmed through spectrometry and high performance liquid chromatographic analysis. Trimethyl phosphate and acetamide, N-acetyl N-methyl was generated by the degradation of monocrotophos using consortium culture, confirmed by the gas chromatography-mass spectrometry analysis. The production of phosphotriesterase enzyme facilitated the degradation of monocrotophos and the molecular weight of phosphotriesterase was found between a range of 35-67 kDa. Degraded metabolites were found to be less toxic than undegraded monocrotophos by cytotoxicity and phytotoxicity tests. Along with monocrotophos degradation and detoxification, isolates also exhibited plant growthpromoting traits. Thus, this screening might be helpful to carry out effective in situ degradation of monocrotophos with the consortium-based formulated prototype for the rectification of polluted sites along with plant growth.

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mentioning

confidence: 51%

Section: Monocrotophos Degradationmentioning

confidence: 98%

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Degradation and detoxification of monocrotophos using bacterial consortium

Jokhakar¹,

Dudhagara²

2022

J App Biol biotech

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“…Screening of Isolates for PGPR Traits: The actinobacterial isolates were screened for PGPR traits [16][17][18][19][20] .…”

Section: Methodsmentioning

confidence: 99%

Untitled

2023

IJPSR

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Bioremediation of organic compounds and heavy metals has been recognized to be a successful and effective technique in rhizoremediation of soil toxicity using the beneficial microbes which produce different metabolites and enzymes to degrade the toxic compounds/ pesticides available in the soil. In the present study, actinobacterial isolates were explored and identified for bioremediation of soil toxicity available in the soil. The actinobacterial isolates were utilized for the promising PGPR traits and ability to degrade the monocrotophos pesticide residues available in the soil. The "opd" gene of interest responsible for monocrotophos pesticide degradation trait was isolated and cloned in vector DNA to produce recombinant DNA. The recombinant DNA was transformed in E. coli cells to produce multiple copies in E. coli cells resulting in recombinant (transformed) and non-recombinant (non-transformed) colonies. The transformed E. coli cells were inoculated in nutrient broth having pesticide concentration. The transformed cells degraded the pesticide, and the HPLC method determined the derivatives produced. The formulations based on actinobacterial isolates were tested, and field applications were done to determine the reduced soil toxicity, if any, observed.

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“…70 Other bacterial strains isolated from pesticide-polluted soil, including Bacillus licheniformis, Bacillus subtilis, Pseudomonas stutzeri, Rhodococcus phenolicus, and Rhococcus ruber have also demonstrated substantial efficiency in breaking down monocrotophos. 71,72 Moreover, Paracoccus sp., isolated from sludge, exhibits a rapid monocrotophos degradation rate, removing approximately 80% of the substance within a mere 6 hours of incubation, and it has been found to also be effective in breaking down the amide group found in certain herbicides. 73 Further, two other effective agents in the degradation of monocrotophos are Clavibacter michiganense subsp.…”

Section: Monocrotophos Breakdownmentioning

confidence: 99%

Advanced pesticide nano formulations and understanding their breakdown by Bacteria

Ahirwar

2023

IJMR

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The widespread use of chemical pesticides in agriculture has undeniably caused significant environmental harm, affecting the quality of air, water, and soil. This growing concern has steered the focus towards the development and research of nano formulations, which promise effective pest control with substantially reduced pesticide concentrations. These new formulations, which allow for controlled delivery of active ingredients, are demonstrating effectiveness comparable to traditional pesticides, but with less environmental impact. However, it is important to acknowledge that these nano-pesticides still contain certain organic groups which are structurally complex and not easily degradable. These elements can persist in the environment, accumulating and becoming more concentrated through the food chain, potentially causing a range of environmental hazards over time. Encouragingly, research has identified that specific bacterial genera including , and and among others, have the unique ability to break down certain chemical groups present in these pesticides, using them as a sole source of carbon or nitrogen. This process transforms them into non-harmful end products, marking a promising step towards bioremediation. Currently, efforts are being made to develop this into a viable large-scale solution, exploring different combinations of nano-pesticides and bacteria strains under optimized conditions. Through further research in this area, we aim to identify the specific types of bacteria that are most effective in degrading these pesticide groups, ultimately paving the way for more sustainable environmental management in the future.

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Biodegradation of monocrotophos by bacteria isolated from soil

Cited by 13 publications

References 31 publications

Degradation and detoxification of monocrotophos using bacterial consortium

Degradation and detoxification of monocrotophos using bacterial consortium

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Advanced pesticide nano formulations and understanding their breakdown by Bacteria

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