Biodegradation of n-alkanes on oil–seawater interfaces at different temperatures and microbial communities associated with the degradation

Lofthus, Synnøve; Netzer, Roman; Lewin, Anna; Heggeset, Tonje Marita Bjerkan; Haugen, Tone; Brakstad, Odd Gunnar

doi:10.1007/s10532-018-9819-z

Cited by 39 publications

(16 citation statements)

References 70 publications

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“…Also the TRD communities were in agreement with recent data from TRD surface seawater was predominated by Rhodobacteraceae, Synechococcus and non-identified groups, in agreement with data from previous studies (Brakstad et al 2015b). Although the TRD seawater was collected at a temperature of 11 °C, we have previously detected typical psychrophilic oil-degrading bacteria in this water (Lofthus et al 2018;Ribicic et al 2018).…”

Section: Discussionsupporting

confidence: 92%

“…Oceanospirillaceae is associated with alkane metabolism (Hazen et al 2010;Redmond and Valentine 2012;Dubinsky et al 2013;Brakstad et al 2015b;Wang et al 2016), in agreement with the abundances early during the oil biodegradation period. This family was represented by the genus Oleispira, known to biodegrade alkanes at low seawater temperatures (Yakimov et al 2003;Coulon et al 2007;Golyshin et al 2010;Lofthus et al 2018). Sulfitobacter and Polaribacter are also associated with oil biodegradation in seawater or with oil-contaminated marine environments (Deppe et al 2005;Brakstad et al 2008Brakstad et al , 2018bGuibert et al 2012;Dubinsky et al 2013;Ribicic et al 2018).…”

Section: Discussionmentioning

confidence: 99%

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Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

et al. 2018

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With increasing oil exploration, production and transportation in the Arctic, predictions of the fate of spilled oil become important, including oil compound biodegradation. The use of chemical dispersants may result in increased biodegradation due to the generation of dispersions consisting of small oil droplets, but only few studies have focused on biodegradation of chemically dispersed oil in Arctic seawater. In this study we compared oil biotransformation in Arctic and temperate seawaters collected from Western Greenland (Disko Bay, surface and from 80 m depth) and a Norwegian fjord (Trondheimsfjord, surface). A naphthenic oil, premixed with a dispersant, was dispersed in the seawaters from the different sources, and the dispersions incubated in low concentrations in a carousel system at 4-5 °C for up to 64 days. Targeted oil compounds (n-alkanes, BTEX, naphthalenes and PAHs) were biotransformed in both Arctic and temperate seawaters, although the degradation was faster in the temperate seawater. In the Arctic seawater, transformation was faster in the surface than in the subsurface seawater. Calculations of biotransformation rates and half-lives of oil compound groups representing 70-80% of fresh oils also showed significantly faster depletion in the temperate than the Arctic seawater. Microbial analyses revealed differences between the bacterial communities in the seawater sources during oil biodegradation. The results emphasized, that oil compounds are biodegraded in Arctic seawater, but degradation potential and rates may vary between seawaters from different sources.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

et al. 2018

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“…Acidaminobacteraceae, Flammeovirgaceae, Methanobacteriaceae, Oceanospirillaceae, Shewanellaceae include bacteria found in marine environments ( Cho et al, 2004 ; Mason et al, 2012 ; Bendall et al, 2013 ; Koo et al, 2015 ; Campeão et al, 2017 ; Dong et al, 2017 ), showing they can tolerate and thrive in high salinity environments. Furthermore, several enriched UOG+ OTUs included taxa encompassing known halophilic hydrocarbon degraders, such as Cyclobacteriaceae ( Wang et al, 2014 ), Oceanospirallaceae ( Lofthus et al, 2018 ), and Shewanellaceae ( Bayat et al, 2015 ). Overall, the increased abundance of several halophilic taxa in UOG+ samples (Supplementary Figure 9 ) potentially suggests those streams may have been impacted by hydraulic fracturing.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Biomarkers of Marcellus Shale Activity in Pennsylvania

et al. 2018

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Unconventional oil and gas (UOG) extraction, also known as hydraulic fracturing, is becoming more prevalent with the increasing use and demand for natural gas; however, the full extent of its environmental impacts is still unknown. Here we measured physicochemical properties and bacterial community composition of sediment samples taken from twenty-eight streams within the Marcellus shale formation in northeastern Pennsylvania differentially impacted by hydraulic fracturing activities. Fourteen of the streams were classified as UOG+, and thirteen were classified as UOG- based on the presence of UOG extraction in their respective watersheds. One stream was located in a watershed that previously had UOG extraction activities but was recently abandoned. We utilized high-throughput sequencing of the 16S rRNA gene to infer differences in sediment aquatic bacterial community structure between UOG+ and UOG- streams, as well as correlate bacterial community structure to physicochemical water parameters. Although overall alpha and beta diversity differences were not observed, there were a plethora of significantly enriched operational taxonomic units (OTUs) within UOG+ and UOG- samples. Our biomarker analysis revealed many of the bacterial taxa enriched in UOG+ streams can live in saline conditions, such as Rubrobacteraceae. In addition, several bacterial taxa capable of hydrocarbon degradation were also enriched in UOG+ samples, including Oceanospirillaceae. Methanotrophic taxa, such as Methylococcales, were significantly enriched as well. Several taxa that were identified as enriched in these samples were enriched in samples taken from different streams in 2014; moreover, partial least squares discriminant analysis (PLS-DA) revealed clustering between streams from the different studies based on the presence of hydraulic fracturing along the second axis. This study revealed significant differences between bacterial assemblages within stream sediments of UOG+ and UOG- streams and identified several potential biomarkers for evaluating and monitoring the response of autochthonous bacterial communities to potential hydraulic fracturing impacts.

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“…Colwellia, Pseudomonas, and Pseudoalteromanas are generalist strains with rapid facultative degrading capabilities toward ADOC, but also prompt to degrade other dissolved organic matter compounds. Colwellia have been described as abundant and fast responder following ADOC background concentration exposures in polar environments (Cerro-Gálvez et al, 2019), and to oil spills in marine environments (Gutierrez et al, 2013;Dombrowski et al, 2016;Lofthus et al, 2018;Vergeynst et al, 2018). In fact, Colwellia single cell assembled genomes (SAGs) retrieved from contaminated marine sites have revealed a more specific metabolic activity toward aromatic hydrocarbons, as well as a full set of genes involved with chemotaxis, motility and adaptations to cold environments such as Antarctica (Mason et al, 2014).…”

Section: Structure Of Bacterioneuston and Bacterioplankton Communitiesmentioning

confidence: 99%

Large Enrichment of Anthropogenic Organic Matter Degrading Bacteria in the Sea-Surface Microlayer at Coastal Livingston Island (Antarctica)

et al. 2020

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The composition of bacteria inhabiting the sea-surface microlayer (SML) is poorly characterized globally and yet undescribed for the Southern Ocean, despite their relevance for the biogeochemistry of the surface ocean. We report the abundances and diversity of bacteria inhabiting the SML and the subsurface waters (SSL) determined from a unique sample set from a polar coastal ecosystem (Livingston Island, Antarctica). From early to late austral summer (January-March 2018), we consistently found a higher abundance of bacteria in the SML than in the SSL. The SML was enriched in some Gammaproteobacteria genus such as Pseudoalteromonas, Pseudomonas, and Colwellia, known to degrade a wide range of semivolatile, hydrophobic, and surfactant-like organic pollutants. Hydrocarbons and other synthetic chemicals including surfactants, such as perfluoroalkyl substances (PFAS), reach remote marine environments by atmospheric transport and deposition and by oceanic currents, and are known to accumulate in the SML. Relative abundances of specific SMLenriched bacterial groups were significantly correlated to concentrations of PFASs, taken as a proxy of hydrophobic anthropogenic pollutants present in the SML and its stability. Our observations provide evidence for an important pollutant-bacteria interaction in the marine SML. Given that pollutant emissions have increased during the Anthropocene, our results point to the need to assess chemical pollution as a factor modulating marine microbiomes in the contemporaneous and future oceans.

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Biodegradation of n-alkanes on oil–seawater interfaces at different temperatures and microbial communities associated with the degradation

Cited by 39 publications

References 70 publications

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord

Bacterial Biomarkers of Marcellus Shale Activity in Pennsylvania

Large Enrichment of Anthropogenic Organic Matter Degrading Bacteria in the Sea-Surface Microlayer at Coastal Livingston Island (Antarctica)

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