Biodegradation of Navy N5RL1 carpet dye by Staphylococcus saprophyticus strain BHUSS X3

Kumari, Lata; Verma, Ajay; Tiwary, Dhanesh; Giri, Deen Dayal; Nath, Gopal; Mishra, Pradeep Kumar

doi:10.1007/s13205-015-0276-7

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…4 b, the NYC-1 consortium decolorized AO7 efficiently (approximately complete decolorization) after 6 h of incubation under static conditions, whereas above 48% decolorization was achieved after 15 h of incubation under agitation conditions (120 rpm). This was probably due to the synergistic metabolic activities and production of enzymes by NYC-1 for the decolorization of AO7 dye under static conditions [ 95 ]. On the other hand, the growth of NYC-1 consortium under agitation conditions was fast as compared to its growth under static conditions, which was probably due to the facultative nature of yeast cells to grow anaerobically.…”

Section: Resultsmentioning

confidence: 99%

Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts

Ali

Al-Tohamy

Koutra

et al. 2021

Biotechnol Biofuels

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Background Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. However, an effective and environmentally friendly treatment method has not yet been implemented, while concurrently, the increasing demand of modern societies for adequate and sustainable energy supply still remains a global challenge. Under this scope, the purpose of the present study was to isolate promising species of yeasts inhabiting wood-feeding termite guts, for combined azo dyes and textile wastewater bioremediation, along with biodiesel production. Results Thirty-eight yeast strains were isolated, molecularly identified and subsequently tested for desired enzymatic activity, lipid accumulation, and tolerance to lignin-derived metabolites. The most promising species were then used for construction of a novel yeast consortium, which was further evaluated for azo dyes degradation, under various culture conditions, dye levels, as well as upon the addition of heavy metals, different carbon and nitrogen sources, and lastly agro-waste as an inexpensive and environmentally friendly substrate alternative. The novel yeast consortium, NYC-1, which was constructed included the manganese-dependent peroxidase producing oleaginous strains Meyerozyma caribbica, Meyerozyma guilliermondii, Debaryomyces hansenii, and Vanrija humicola, and showed efficient azo dyes decolorization, which was further enhanced depending on the incubation conditions. Furthermore, enzymatic activity, fatty acid profile and biodiesel properties were thoroughly investigated. Lastly, a dye degradation pathway coupled to biodiesel production was proposed, including the formation of phenol-based products, instead of toxic aromatic amines. Conclusion In total, this study might be the first to explore the application of MnP and lipid-accumulating yeasts for coupling dye degradation and biodiesel production.

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Section: Resultsmentioning

confidence: 99%

Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts

Ali

Al-Tohamy

Koutra

et al. 2021

Biotechnol Biofuels

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“…strain MEH038S. The maximum decolorization percentage of Navy N5RL1 by Staphylococcus saprophytic BHUSS X3 was observed at 35 ° C (Kumari et al, 2015). The optimal temperature for decolorization of Reactive Blue 4 by Staphylococcus Hominis Subsp.…”

Section: Resultsmentioning

confidence: 99%

Decolorization mechanism, identification of an FMN‐dependent NADH‐azoreductase from a moderately halotolerant Staphylococcus sp. MEH038S, and toxicity assessment of biotransformed metabolites

Fazeliyan

Sadeghi

Forouzandeh

et al. 2021

Water Environment Research

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The application of halotolerant microorganisms capable of decolorizing is attractive. Decolorization mechanism, the effect of different parameters on the decolorization percentage, and toxicity analysis of Reactive Black 5 before and after decolorization were investigated in the present study. The decolorization percentage for live cells of Staphylococcus sp. strain MEH038S was more than dead cells, which demonstrated that Reactive Black 5 was decolorized through the degradation process. The results confirmed that an FMN‐dependent NADH‐azoreductase gene was responsible for the decolorization and then was identified as Staphylococcus sp. EFS01 azoreductase from a moderately halotolerant Staphylococcus strain for the first time. The maximal decolorization of 98.15% was observed at pH 6.5 and 35 °C for 50 mg/L of Reactive Black 5. In addition, more than 90% decolorization was achieved with 5–40 g/L of NaCl. The results of Gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy showed that Reactive Black 5 was broken to the lower molecular weight compounds without any chromophoric azo groups. Phytotoxicity and fish toxicity proved that the biotransformed metabolites of Reactive Black 5 degradation were more toxic than the original dye. The moderate halotolerant strain exhibited a remarkable decolorization capability and can be applied for textile wastewater treatment. Practitioner Points An azoreductase gene from a moderately halotolerant Staphylococcus was identified. More than 90% decolorization efficiency was observed under high‐salt conditions. Biotransformed metabolites of RB5 degradation were identified. Toxicity analysis of biotransformed metabolites was investigated.

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“…16S rDNA sequence analysis was performed for molecular identification of stain DB2. The genomic DNA was isolated using the modified CTAB method ( Kumari et al, 2015 ). The partial sequence of 16S rDNA was amplified by PCR using bacterial universal primer pairs of 16SF (5′-AGAGTTTGATCATGGCTCAG-3′) and 16SR (5′-ACGGTTACCTTGTTACGACTT-3′; Weisburg et al, 1991 ).…”

Section: Methodsmentioning

confidence: 99%

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

Luan

Cui

et al. 2023

Front. Microbiol.

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IntroductionBipolaris sorokiniana is the popular pathogenic fungi fungus which lead to common root rot and leaf spot on wheat. Generally, chemical fungicides are used to control diseases. However, the environmental pollution resulting from fungicides should not be ignored. It is important to study the mode of antagonistic action between biocontrol microbes and plant pathogens to design efficient biocontrol strategies.ResultsAn antagonistic bacterium DB2 was isolated and identified as Bacillus amyloliquefaciens. The inhibition rate of cell-free culture filtrate (CF, 20%, v/v) of DB2 against B. sorokiniana reached 92.67%. Light microscopy and scanning electron microscopy (SEM) showed that the CF significantly altered the mycelial morphology of B. sorokiniana and disrupted cellular integrity. Fluorescence microscopy showed that culture filtrate destroyed mycelial cell membrane integrity, decreased the mitochondrial transmembrane potential, induced reactive oxygen species (ROS) accumulation, and nuclear damage which caused cell death in B. sorokiniana. Moreover, the strain exhibited considerable production of protease and amylase, and showed a significant siderophore and indole-3-acetic acid (IAA) production. In the detached leaves and potted plants control assay, B. amyloliquefacien DB2 had remarkable inhibition activity against B. sorokiniana and the pot control efficacy was 75.22%. Furthermore, DB2 suspension had a significant promotion for wheat seedlings growth.ConclusionB. amyloliquefaciens DB2 can be taken as a potential biocontrol agent to inhibit B. sorokiniana on wheat and promote wheat growth.

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Biodegradation of Navy N5RL1 carpet dye by Staphylococcus saprophyticus strain BHUSS X3

Cited by 11 publications

References 29 publications

Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts

Coupling azo dye degradation and biodiesel production by manganese-dependent peroxidase producing oleaginous yeasts isolated from wood-feeding termite gut symbionts

Decolorization mechanism, identification of an FMN‐dependent NADH‐azoreductase from a moderately halotolerant Staphylococcus sp. MEH038S, and toxicity assessment of biotransformed metabolites

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

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